FastQCFastQC Report
Thu 2 Feb 2017
SRR4064241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881711
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28370.32176075834372037No Hit
TATCAACGCAGAGTACTTTTTTTTT21140.2397611008595787No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19860.22524387242531851No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13740.1558333739740119No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12560.14245030401117828No Hit
GTACATGGGGTGGTATCAACGCAAA10800.12248911491407048No Hit
GGTATCAACGCAGAGTACTTTTTTT10250.1162512433212243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG855.638867E-1116.76826117
ATCGCCA953.0377123E-1015.00318116
GCGTTAT704.444646E-714.9241141
GTATAGA704.444646E-714.9241141
CGCATCG1006.566552E-1014.25221413
GCGCCGA551.9551141E-413.82111419
TCGAACT707.2381317E-613.57430719
GCATCGC1051.364242E-913.57353714
CGAGCCG1550.013.48749615
CTAGCGG500.001496255213.30282118
GTACTAG500.001502025613.2960291
AGGTTCG655.4480242E-513.153657
GTCCTAC2750.013.1233531
GTCCTAA1850.012.8340041
CCGCATC1207.366907E-1012.66863512
CAGTCTA604.0894153E-412.6664789
CGCAATA604.1090365E-412.6592932
CTTGTAC701.0940681E-412.2092533
CGTTATT701.0958076E-412.2071762
TCCTAAA1950.012.1723982
Produced by FastQC (version 0.11.5)