##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	881711
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15645942945024	32.0	32.0	32.0	32.0	32.0
2	31.139938142996968	32.0	32.0	32.0	32.0	32.0
3	31.328299181931495	32.0	32.0	32.0	32.0	32.0
4	31.45431439553323	32.0	32.0	32.0	32.0	32.0
5	31.346387875392278	32.0	32.0	32.0	32.0	32.0
6	34.915574377545475	36.0	36.0	36.0	36.0	36.0
7	34.960521077768114	36.0	36.0	36.0	36.0	36.0
8	34.88791111826891	36.0	36.0	36.0	32.0	36.0
9	35.047624448373675	36.0	36.0	36.0	36.0	36.0
10	34.853544982426214	36.0	36.0	36.0	32.0	36.0
11	35.048862949424475	36.0	36.0	36.0	36.0	36.0
12	34.933113004147614	36.0	36.0	36.0	32.0	36.0
13	34.999853693557185	36.0	36.0	36.0	36.0	36.0
14	34.93279884225103	36.0	36.0	36.0	32.0	36.0
15	34.88376009826349	36.0	36.0	36.0	32.0	36.0
16	34.9031973061468	36.0	36.0	36.0	32.0	36.0
17	34.87205558283837	36.0	36.0	36.0	32.0	36.0
18	34.863501759646866	36.0	36.0	36.0	32.0	36.0
19	34.86099867190043	36.0	36.0	36.0	32.0	36.0
20	34.84510911171574	36.0	36.0	36.0	32.0	36.0
21	34.837779045514914	36.0	36.0	36.0	32.0	36.0
22	34.83447637604612	36.0	36.0	36.0	32.0	36.0
23	34.77322161116284	36.0	36.0	36.0	32.0	36.0
24	34.74856046936014	36.0	36.0	36.0	32.0	36.0
25	34.72556086971808	36.0	36.0	36.0	32.0	36.0
26	34.65291234883085	36.0	36.0	36.0	32.0	36.0
27	34.66151040420274	36.0	36.0	36.0	32.0	36.0
28	34.629824284828025	36.0	36.0	36.0	32.0	36.0
29	34.604442952395964	36.0	36.0	36.0	32.0	36.0
30	34.583852305347214	36.0	36.0	36.0	32.0	36.0
31	34.57031612399074	36.0	36.0	36.0	32.0	36.0
32	34.51671919710653	36.0	36.0	36.0	32.0	36.0
33	34.47627170354005	36.0	36.0	36.0	32.0	36.0
34	34.465669590149155	36.0	36.0	36.0	32.0	36.0
35	34.421742498392334	36.0	36.0	36.0	32.0	36.0
36	34.38887685420733	36.0	36.0	36.0	32.0	36.0
37	34.371844062283444	36.0	36.0	36.0	32.0	36.0
38	34.31925767059728	36.0	36.0	36.0	32.0	36.0
39	34.29510349763131	36.0	36.0	36.0	32.0	36.0
40	34.28448550602182	36.0	36.0	36.0	32.0	36.0
41	34.24667946753528	36.0	36.0	36.0	32.0	36.0
42	34.186332029429146	36.0	36.0	36.0	32.0	36.0
43	34.17574125762296	36.0	36.0	36.0	32.0	36.0
44	34.092668686224854	36.0	36.0	36.0	32.0	36.0
45	34.04726832261365	36.0	36.0	36.0	32.0	36.0
46	34.00698528202552	36.0	36.0	36.0	32.0	36.0
47	33.9730841511561	36.0	36.0	36.0	32.0	36.0
48	33.90831689748682	36.0	36.0	36.0	32.0	36.0
49	33.87660015583337	36.0	36.0	36.0	32.0	36.0
50	33.21160108017253	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	6.0
21	24.0
22	96.0
23	279.0
24	675.0
25	1611.0
26	3423.0
27	6526.0
28	11017.0
29	17468.0
30	25633.0
31	37640.0
32	55791.0
33	92006.0
34	181938.0
35	447576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.087494357068806	18.65876316022295	12.288546040647253	25.96519644206099
2	15.054870773544076	21.154962867525303	38.40114867960821	25.389017679322407
3	17.897494641095143	25.597078404464053	29.431899377346294	27.07352757709451
4	11.574654280143948	16.655797647982162	37.31653569026586	34.45301238160803
5	13.367985655163652	38.02980795294603	34.051520282723025	14.550686109167291
6	32.88537701824187	37.3425215604259	17.108838753873165	12.663262667459069
7	28.712161595082282	31.429948622562975	21.60256773769153	18.25532204466321
8	27.108428952343793	34.48681030405654	19.714282797878216	18.690477945721444
9	27.207265039661927	13.718353820224975	18.622845360450583	40.45153577966252
10	14.627551587490615	28.1693671133008	33.20301778597401	24.000063513234572
11	36.92354977991655	21.14479687788856	23.162124551015015	18.769528791179876
12	23.815172998862437	24.21156138462603	30.516121495592092	21.457144120919438
13	29.445802918880176	20.23969386690378	25.733916443992793	24.58058677022325
14	22.71721994760182	20.097877987093263	26.714792845720247	30.470109219584675
15	24.318254768502083	28.29955845207566	23.264800588881794	24.11738619054046
16	24.419676831419054	26.360571028697745	25.311129434154427	23.908622705728774
17	22.876997111298373	26.503128576143432	26.384722431726495	24.2351518808317
18	23.509597281639728	25.901999301356003	27.74206426617429	22.84633915082998
19	24.792023690302152	25.4299878304796	26.437687632342115	23.34030084687613
20	24.911563190779663	25.18966137123272	26.328139974571936	23.570635463415684
21	25.98606572902005	24.87050745652487	25.592059076046457	23.551367738408615
22	24.69471289345375	25.361938322194007	26.213124254999652	23.73022452935259
23	23.76152168464127	25.04471440058886	26.46922390317872	24.724540011591152
24	23.947692255346176	25.75589341106152	26.39102647710969	23.90538785648261
25	24.21384646443567	25.342123947201877	26.44885210128638	23.995177487076074
26	23.454713524978306	26.189992797717952	27.001412100968054	23.353881576335684
27	24.025951194061168	25.564194611270846	26.41203191724654	23.99782227742144
28	23.39578985572997	25.958057345068507	26.717062879956448	23.92908991924508
29	23.629754390740455	25.38344193220935	26.587159813312013	24.39964386373818
30	23.51179843141258	26.048078395345275	26.649540368725795	23.79058280451635
31	24.13930777126848	25.64161312092736	26.013418099121527	24.205661008682632
32	23.698820775314427	26.149707207028396	25.92388857194382	24.227583445713357
33	23.639776561658206	25.554200810956416	26.46644171605183	24.33958091133354
34	23.921613994973278	26.000580704285415	26.638788324214463	23.439016976526844
35	24.48983758279648	25.602486162780146	26.578010162417204	23.32966609200617
36	23.613986096476257	26.204932359549797	26.159449303406014	24.021632240567936
37	24.161487160874696	26.148942927139096	25.883994192904453	23.805575719081755
38	23.901851429660304	25.96084809837996	26.198132350622643	23.939168121337097
39	24.296848948704397	25.480052717898	26.092297152915933	24.130801180481676
40	24.749174880059886	26.096473817328086	26.07844026812145	23.07591103449058
41	23.631995971354883	25.84242009059915	26.80512109753359	23.720462840512383
42	24.573889413131056	26.617337495009618	25.98061009690415	22.82816299495518
43	23.912976907032686	25.794915940510176	26.316070033129296	23.97603711932784
44	23.661709215007775	26.260997183858965	25.82037261980508	24.25692098132818
45	23.80821140977657	26.474991493705343	25.761823749574685	23.954973346943405
46	23.554390135464963	26.10785479026975	25.70170941721938	24.6360456570459
47	24.054471857303422	25.99412727415014	26.012273974342836	23.939126894203604
48	24.33780880895186	26.357557157018196	25.28791167910863	24.01672235492132
49	24.0461967524359	26.478750877559097	25.492141947381615	23.982910422623387
50	24.070686254606162	26.603523424660796	25.20451990985586	24.121270410877184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	23.5
2	21.0
3	72.0
4	123.0
5	142.5
6	162.0
7	168.5
8	175.0
9	265.5
10	356.0
11	515.0
12	674.0
13	1262.0
14	1850.0
15	2521.5
16	3193.0
17	3567.0
18	3941.0
19	4056.0
20	4171.0
21	4676.5
22	5182.0
23	5887.5
24	6593.0
25	7534.5
26	8476.0
27	10515.0
28	12554.0
29	14312.0
30	16070.0
31	17940.5
32	19811.0
33	22856.0
34	25901.0
35	29342.0
36	32783.0
37	36506.5
38	40230.0
39	41941.5
40	43653.0
41	46280.0
42	48907.0
43	49856.5
44	50806.0
45	54225.5
46	57645.0
47	60776.5
48	63908.0
49	65159.0
50	66410.0
51	63379.5
52	60349.0
53	58567.5
54	56786.0
55	56111.0
56	55436.0
57	53506.5
58	51577.0
59	46627.5
60	41678.0
61	36555.0
62	31432.0
63	26956.0
64	22480.0
65	18951.0
66	15422.0
67	13183.0
68	10944.0
69	9851.5
70	8759.0
71	6768.5
72	4778.0
73	4005.0
74	3232.0
75	2526.5
76	1821.0
77	1578.0
78	1335.0
79	1084.5
80	834.0
81	677.0
82	520.0
83	426.0
84	332.0
85	238.5
86	145.0
87	106.0
88	67.0
89	56.0
90	45.0
91	41.5
92	38.0
93	30.5
94	23.0
95	24.0
96	25.0
97	20.5
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008733020229984654
2	3.4024754142797354E-4
3	1.1341584714265786E-4
4	0.0
5	0.0
6	3.4024754142797354E-4
7	1.1341584714265786E-4
8	0.0
9	0.0010207426242839208
10	5.670792357132892E-4
11	0.0
12	0.0
13	3.4024754142797354E-4
14	1.1341584714265786E-4
15	0.004536633885706314
16	4.5366338857063144E-4
17	0.0
18	3.4024754142797354E-4
19	0.0
20	9.073267771412629E-4
21	0.0
22	0.0
23	4.5366338857063144E-4
24	6.804950828559471E-4
25	0.001474406012854552
26	0.005217128968562261
27	0.00635128743998884
28	0.0035158912614223934
29	0.002948812025709104
30	0.002948812025709104
31	0.007485445911415419
32	0.0034024754142797354
33	0.004082970497135683
34	0.0026085644842811307
35	0.0035158912614223934
36	0.007258614217130103
37	0.0035158912614223934
38	0.0077122776057007335
39	0.004196386344278341
40	0.002381732789995815
41	0.0028353961785664464
42	0.0017012377071398677
43	0.00158782185999721
44	9.073267771412629E-4
45	0.0012475743185692363
46	7.93910929998605E-4
47	9.073267771412629E-4
48	0.0019280694014251834
49	4.5366338857063144E-4
50	0.0013609901657118941
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	881711.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.15566254298851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86721353049722	48.398882473644136
2	13.979194709023826	17.377722179136306
3	4.150242542304682	7.7388322479313345
4	1.6491872226808986	4.100252979326495
5	0.7798627354074171	2.4236442505917672
6	0.41435550616607486	1.5452724608452646
7	0.2441179889563579	1.0621320739571065
8	0.16312262198909674	0.811119571638542
9	0.11282576965545904	0.6311484418371898
>10	0.5253900935838002	6.1363349090819685
>50	0.06256712665592604	2.767677913397117
>100	0.04898473812350145	5.910668069297547
>500	0.0029354149558796397	1.0963124293152147
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1341584714265786E-4	0.0	0.0	0.0
6	0.0	1.1341584714265786E-4	0.0	0.0	0.0
7	0.0	1.1341584714265786E-4	0.0	0.0	0.0
8	0.0	1.1341584714265786E-4	0.0	0.0	0.0
9	0.0	1.1341584714265786E-4	0.0	0.0	0.0
10	0.0	1.1341584714265786E-4	0.0	0.0	0.0
11	0.0	1.1341584714265786E-4	0.0	0.0	0.0
12	0.0	1.1341584714265786E-4	0.0	0.0	2.2683169428531572E-4
13	0.0	1.1341584714265786E-4	0.0	0.0	3.402475414279736E-4
14	0.0	1.1341584714265786E-4	0.0	0.0	3.402475414279736E-4
15	0.0	1.1341584714265786E-4	0.0	0.0	5.670792357132892E-4
16	0.0	1.1341584714265786E-4	0.0	1.1341584714265786E-4	5.670792357132892E-4
17	0.0	1.1341584714265786E-4	0.0	1.1341584714265786E-4	5.670792357132892E-4
18	0.0	1.1341584714265786E-4	0.0	1.1341584714265786E-4	5.670792357132892E-4
19	0.0	1.1341584714265786E-4	0.0	1.1341584714265786E-4	5.670792357132892E-4
20	0.0	2.2683169428531572E-4	0.0	1.1341584714265786E-4	6.804950828559472E-4
21	0.0	2.2683169428531572E-4	0.0	1.1341584714265786E-4	7.93910929998605E-4
22	0.0	2.2683169428531572E-4	0.0	1.1341584714265786E-4	7.93910929998605E-4
23	0.0	2.2683169428531572E-4	0.0	0.0010207426242839206	7.93910929998605E-4
24	0.0	2.2683169428531572E-4	0.0	0.0012475743185692363	7.93910929998605E-4
25	0.0	2.2683169428531572E-4	0.0	0.00158782185999721	7.93910929998605E-4
26	0.0	2.2683169428531572E-4	0.0	0.0019280694014251836	0.0010207426242839206
27	0.0	2.2683169428531572E-4	0.0	0.0021549010957104993	0.0010207426242839206
28	0.0	2.2683169428531572E-4	0.0	0.0032890595671370777	0.0010207426242839206
29	0.0	2.2683169428531572E-4	0.0	0.005557376509990235	0.0010207426242839206
30	0.0	2.2683169428531572E-4	0.0	0.010207426242839207	0.0010207426242839206
31	0.0	2.2683169428531572E-4	0.0	0.01848678308425323	0.0010207426242839206
32	0.0	2.2683169428531572E-4	0.0	0.03107594211708825	0.0010207426242839206
33	0.0	2.2683169428531572E-4	0.0	0.04173703174849809	0.0010207426242839206
34	0.0	2.2683169428531572E-4	0.0	0.05285178476847856	0.0010207426242839206
35	0.0	3.402475414279736E-4	0.0	0.06975074599273458	0.0010207426242839206
36	0.0	3.402475414279736E-4	0.0	0.09129975694983958	0.0010207426242839206
37	0.0	4.5366338857063144E-4	0.0	0.1240769367740677	0.0010207426242839206
38	0.0	4.5366338857063144E-4	0.0	0.16932985978398818	0.0010207426242839206
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	175	0.0	22.628279	44
CGGGATT	90	4.0797204E-7	21.999716	44
TAGTACC	70	3.215808E-5	21.99847	4
ACGGGAT	95	6.8628106E-7	20.841837	43
GGCGTTA	180	0.0	20.77751	42
GCGTTAT	180	0.0	20.77751	43
GTACTAG	65	4.927364E-4	20.309736	1
GATATAC	370	0.0	20.218248	1
TAGGACG	290	0.0	19.722765	4
CGGCGTT	190	0.0	19.683958	41
ACGGTAT	205	0.0	19.316824	9
ACGACGG	205	0.0	19.31573	6
TACGACG	195	0.0	19.178152	5
GCGGCGT	210	0.0	18.856901	40
CGACGGT	210	0.0	18.855831	7
TTATACT	140	4.0799932E-9	18.855831	4
GACGGTA	200	0.0	18.698698	8
AGGACGT	310	0.0	18.450329	5
GTAATAC	85	1.4310388E-4	18.116386	3
TAGGACA	245	0.0	17.957933	4
>>END_MODULE