FastQCFastQC Report
Thu 2 Feb 2017
SRR4064240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303728
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34500.2646257501564744No Hit
TATCAACGCAGAGTACTTTTTTTTT22410.17189168292772725No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13670.10485315955475376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA801.2854798E-714.2521616
AAGACCG400.00528260214.248335
GGTCCAA2500.014.06159111
TCGCGTA551.9585267E-413.8192159
CCGTCCG707.246159E-613.57400618
AAGACGG2850.012.6651825
TTAATAC751.4834381E-512.6622673
CAAGACG2800.012.5511374
CGCAAGA2550.012.2893742
GTCCTAA853.960671E-612.2889031
TCTACGA701.09457476E-412.2095742
GACGGAC2900.012.1197367
CGGACAT1650.012.0894935
AGTAGTG550.003070681612.0894935
CTACACG550.003072447612.0885654
CTTACTC550.00307598212.0867083
GGTCATA550.003077750612.0857811
AGACGGA3000.012.0319246
ACGAACG951.0374315E-612.0018215
GTCCTAT3100.011.9465461
Produced by FastQC (version 0.11.5)