##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064240_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1303728 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193625510842754 32.0 32.0 32.0 32.0 32.0 2 30.769475688180357 32.0 32.0 32.0 32.0 32.0 3 30.779949498668433 32.0 32.0 32.0 32.0 32.0 4 30.820128124884945 32.0 32.0 32.0 32.0 32.0 5 30.705657928647693 32.0 32.0 32.0 32.0 32.0 6 34.35779625811519 36.0 36.0 36.0 32.0 36.0 7 34.28000395788079 36.0 36.0 36.0 32.0 36.0 8 34.23664982266239 36.0 36.0 36.0 32.0 36.0 9 34.39111225654431 36.0 36.0 36.0 32.0 36.0 10 34.065344918571974 36.0 36.0 36.0 32.0 36.0 11 34.37968272523103 36.0 36.0 36.0 32.0 36.0 12 34.174954438349104 36.0 36.0 36.0 32.0 36.0 13 34.26720681000945 36.0 36.0 36.0 32.0 36.0 14 34.15276269283164 36.0 36.0 36.0 32.0 36.0 15 34.08738555895095 36.0 36.0 36.0 32.0 36.0 16 34.08747760269013 36.0 36.0 36.0 32.0 36.0 17 34.01264834382632 36.0 36.0 36.0 32.0 36.0 18 34.004459519163504 36.0 36.0 36.0 32.0 36.0 19 34.031293337260536 36.0 36.0 36.0 32.0 36.0 20 34.01442325393027 36.0 36.0 36.0 32.0 36.0 21 33.996015273124456 36.0 36.0 36.0 32.0 36.0 22 33.96623222021771 36.0 36.0 36.0 32.0 36.0 23 33.915026754046856 36.0 36.0 36.0 32.0 36.0 24 33.905612213590565 36.0 36.0 36.0 32.0 36.0 25 33.50050547353435 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 9.0 5 11.0 6 46.0 7 27.0 8 70.0 9 82.0 10 124.0 11 37.0 12 77.0 13 84.0 14 120.0 15 216.0 16 380.0 17 465.0 18 599.0 19 889.0 20 1301.0 21 2048.0 22 3169.0 23 4904.0 24 7326.0 25 10830.0 26 15903.0 27 20780.0 28 28436.0 29 37968.0 30 50127.0 31 68451.0 32 95321.0 33 132086.0 34 272649.0 35 549189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.667361591210955 17.219601015607797 11.3721831193953 25.740854273785946 2 16.94653279730359 19.277050210216014 37.267260840449836 26.509156152030556 3 18.787369286598025 23.357937456997753 28.762662890778707 29.09203036562551 4 12.536837858195915 15.007831640441028 36.01005150013117 36.44527900123189 5 14.450113261272623 36.592020852654485 33.69877641005107 15.259089476021826 6 34.20429849344839 35.82323352815685 16.580889601119388 13.391578377275382 7 30.382716570319424 30.63658228770681 20.500826655516725 18.479874486457042 8 28.495707359218343 32.672332896230046 19.049902668427357 19.782057076124264 9 27.1976333268104 13.975343317256225 18.66471746112141 40.162305894811965 10 15.70014014007868 26.754859238270367 31.629907397032348 25.91509322461861 11 37.29618345216076 21.36568406996109 22.115025978311756 19.223106499566388 12 24.447975070708466 23.807780425747477 28.893417300836223 22.850827202707837 13 29.088141790712562 19.640672419059957 25.3482674509816 25.922918339245886 14 23.345573279256516 19.62055144231286 25.277586315412968 31.75628896301766 15 25.306981424160487 26.965782479321593 22.47559153261613 25.25164456390179 16 25.788944075637314 26.0365552723301 23.546704184445012 24.627796467587572 17 23.847172280404173 25.867120012587257 25.25118868988913 25.03451901711944 18 24.547870462200745 25.23734881678698 25.900420964072207 24.314359756940075 19 25.351446700001073 25.049548726624593 25.34453827955768 24.254466293816655 20 25.57319061524148 24.68066519895479 24.978199258774797 24.76794492702893 21 26.66848847348964 24.12676090934771 24.768333722258383 24.436416894904266 22 25.71169992769009 24.280316812591444 25.077951925424074 24.930031334294394 23 24.583680378301406 24.09330104055916 25.595145336675202 25.727873244464234 24 24.570511363985286 25.300143086115735 25.160971880200687 24.96837366969829 25 24.872709519785005 24.609185793822046 25.27371387295439 25.24439081343856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 95.0 2 235.0 3 375.0 4 375.0 5 375.0 6 1306.0 7 2237.0 8 2237.0 9 2237.0 10 2469.5 11 2702.0 12 2702.0 13 2702.0 14 3301.0 15 3900.0 16 3900.0 17 3900.0 18 6347.5 19 8795.0 20 8795.0 21 8795.0 22 14002.0 23 19209.0 24 19209.0 25 19209.0 26 28721.0 27 38233.0 28 38233.0 29 38233.0 30 47028.5 31 55824.0 32 55824.0 33 55824.0 34 68336.0 35 80848.0 36 80848.0 37 80848.0 38 93618.0 39 106388.0 40 106388.0 41 106388.0 42 119962.0 43 133536.0 44 133536.0 45 133536.0 46 151052.5 47 168569.0 48 168569.0 49 168569.0 50 172365.0 51 176161.0 52 176161.0 53 176161.0 54 165406.0 55 154651.0 56 154651.0 57 154651.0 58 140849.5 59 127048.0 60 127048.0 61 127048.0 62 111367.0 63 95686.0 64 95686.0 65 95686.0 66 79024.5 67 62363.0 68 62363.0 69 62363.0 70 47245.5 71 32128.0 72 32128.0 73 32128.0 74 25112.5 75 18097.0 76 18097.0 77 18097.0 78 13966.0 79 9835.0 80 9835.0 81 9835.0 82 6680.5 83 3526.0 84 3526.0 85 3526.0 86 2643.0 87 1760.0 88 1760.0 89 1760.0 90 1238.0 91 716.0 92 716.0 93 716.0 94 502.5 95 289.0 96 289.0 97 289.0 98 523.0 99 757.0 100 757.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03597376139808304 2 0.0052925150031294865 3 0.001303952971785526 4 0.0032215308714701224 5 0.008053827178675307 6 0.0118889829780445 7 0.021706981824429636 8 0.034593105310310124 9 0.04855307242001399 10 0.057220524526588365 11 0.05507283727894162 12 0.0646607267773646 13 0.06519764858927629 14 0.06826577322877166 15 0.06128578967391972 16 0.06742203895291042 17 0.06312666445761693 18 0.0770099284513334 19 0.07440202250776236 20 0.07823717830713155 21 0.0760894910594848 22 0.07685652221935864 23 0.08153541229458906 24 0.07793036584318201 25 0.07662641287139649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1303728.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.555402740324034 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11122138639888 41.297225171468796 2 13.49488878851236 14.45448408009326 3 4.216647199709789 6.77472716982952 4 1.80198061598231 3.8602319047675913 5 0.9379628523881762 2.511648915755594 6 0.5311929896776711 1.7068952697014683 7 0.3563852287932282 1.3360448121106703 8 0.24466947602078143 1.0482697861245605 9 0.17736332949011935 0.8548888087987319 >10 0.9402448689408353 9.229712201763997 >50 0.09169113311706158 3.4415150627044215 >100 0.08683828223851221 9.463083202576865 >500 0.006757272424556882 2.449830785170808 >1k 0.002156576305709643 1.5714428291338285 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3450 0.2646257501564744 No Hit TATCAACGCAGAGTACTTTTTTTTT 2241 0.17189168292772725 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1367 0.10485315955475376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3010934796215162E-4 2 0.0 0.0 0.0 0.0 2.3010934796215162E-4 3 0.0 0.0 0.0 0.0 2.3010934796215162E-4 4 0.0 0.0 0.0 0.0 2.3010934796215162E-4 5 0.0 0.0 0.0 7.670311598738387E-5 2.3010934796215162E-4 6 0.0 0.0 0.0 7.670311598738387E-5 3.068124639495355E-4 7 0.0 0.0 0.0 1.5340623197476774E-4 3.068124639495355E-4 8 0.0 0.0 0.0 1.5340623197476774E-4 3.068124639495355E-4 9 0.0 0.0 0.0 1.5340623197476774E-4 3.068124639495355E-4 10 0.0 0.0 0.0 1.5340623197476774E-4 3.068124639495355E-4 11 0.0 0.0 0.0 1.5340623197476774E-4 3.068124639495355E-4 12 0.0 0.0 0.0 2.3010934796215162E-4 4.6021869592430324E-4 13 0.0 0.0 0.0 2.3010934796215162E-4 4.6021869592430324E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACGA 80 1.2854798E-7 14.25216 16 AAGACCG 40 0.005282602 14.24833 5 GGTCCAA 250 0.0 14.061591 11 TCGCGTA 55 1.9585267E-4 13.819215 9 CCGTCCG 70 7.246159E-6 13.574006 18 AAGACGG 285 0.0 12.665182 5 TTAATAC 75 1.4834381E-5 12.662267 3 CAAGACG 280 0.0 12.551137 4 CGCAAGA 255 0.0 12.289374 2 GTCCTAA 85 3.960671E-6 12.288903 1 TCTACGA 70 1.09457476E-4 12.209574 2 GACGGAC 290 0.0 12.119736 7 CGGACAT 165 0.0 12.089493 5 AGTAGTG 55 0.0030706816 12.089493 5 CTACACG 55 0.0030724476 12.088565 4 CTTACTC 55 0.003075982 12.086708 3 GGTCATA 55 0.0030777506 12.085781 1 AGACGGA 300 0.0 12.031924 6 ACGAACG 95 1.0374315E-6 12.00182 15 GTCCTAT 310 0.0 11.946546 1 >>END_MODULE