FastQCFastQC Report
Thu 2 Feb 2017
SRR4064240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303728
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25400.021.7400051
AATACGA3300.021.33318539
CGCAATA3300.020.66810836
CAATACG3300.020.66652338
CGAATGC3250.020.3067743
ATACGAA3500.020.11337340
GTACGGA1101.2659257E-719.99986312
TACGGAC1152.0218431E-719.130313
CTAGCGG3350.019.04537429
TACTATA1901.8189894E-1218.5254762
TAGACCG600.00741270618.3332045
TTAGAAC2900.018.206773
TACGAAT3800.017.94655641
GGTATCA12750.017.7724251
GTATTAG3600.017.72211
TCTAGCG3650.017.48000128
ATTACAC2201.8189894E-1216.9998823
GGCGCAA6000.016.86784434
CGGCGCA6200.016.67858333
TAGCGGC3900.016.36074330
Produced by FastQC (version 0.11.5)