##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1303728
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29147720997018	32.0	32.0	32.0	32.0	32.0
2	31.359049587030423	32.0	32.0	32.0	32.0	32.0
3	31.44399905501761	32.0	32.0	32.0	32.0	32.0
4	31.53604969748291	32.0	32.0	32.0	32.0	32.0
5	31.477402494998955	32.0	32.0	32.0	32.0	32.0
6	35.05325727452352	36.0	36.0	36.0	36.0	36.0
7	35.066974859786704	36.0	36.0	36.0	36.0	36.0
8	35.01316378876575	36.0	36.0	36.0	36.0	36.0
9	35.13690969281936	36.0	36.0	36.0	36.0	36.0
10	34.97362870169238	36.0	36.0	36.0	36.0	36.0
11	35.131283519261686	36.0	36.0	36.0	36.0	36.0
12	35.033152620792066	36.0	36.0	36.0	36.0	36.0
13	35.07585017733761	36.0	36.0	36.0	36.0	36.0
14	35.01714621454782	36.0	36.0	36.0	36.0	36.0
15	34.99097817949757	36.0	36.0	36.0	36.0	36.0
16	35.00076012787944	36.0	36.0	36.0	36.0	36.0
17	34.96423026889045	36.0	36.0	36.0	36.0	36.0
18	34.96238632598211	36.0	36.0	36.0	36.0	36.0
19	34.96399939251132	36.0	36.0	36.0	36.0	36.0
20	34.95586886221666	36.0	36.0	36.0	36.0	36.0
21	34.949131260508324	36.0	36.0	36.0	36.0	36.0
22	34.940713093528714	36.0	36.0	36.0	36.0	36.0
23	34.88492461617761	36.0	36.0	36.0	32.0	36.0
24	34.85276376667526	36.0	36.0	36.0	32.0	36.0
25	34.82218837058036	36.0	36.0	36.0	32.0	36.0
26	34.76328497968902	36.0	36.0	36.0	32.0	36.0
27	34.758456518537606	36.0	36.0	36.0	32.0	36.0
28	34.74383613759925	36.0	36.0	36.0	32.0	36.0
29	34.721034602309686	36.0	36.0	36.0	32.0	36.0
30	34.69736018556018	36.0	36.0	36.0	32.0	36.0
31	34.69528690033504	36.0	36.0	36.0	32.0	36.0
32	34.65460126652185	36.0	36.0	36.0	32.0	36.0
33	34.62744146018188	36.0	36.0	36.0	32.0	36.0
34	34.624775259870155	36.0	36.0	36.0	32.0	36.0
35	34.58141805652713	36.0	36.0	36.0	32.0	36.0
36	34.5562931838543	36.0	36.0	36.0	32.0	36.0
37	34.55015846863763	36.0	36.0	36.0	32.0	36.0
38	34.51269053054011	36.0	36.0	36.0	32.0	36.0
39	34.519838493918975	36.0	36.0	36.0	32.0	36.0
40	34.49334677171925	36.0	36.0	36.0	32.0	36.0
41	34.47539670851589	36.0	36.0	36.0	32.0	36.0
42	34.41280543180786	36.0	36.0	36.0	32.0	36.0
43	34.41036243756366	36.0	36.0	36.0	32.0	36.0
44	34.3380152915332	36.0	36.0	36.0	32.0	36.0
45	34.30201008185757	36.0	36.0	36.0	32.0	36.0
46	34.28078249450806	36.0	36.0	36.0	32.0	36.0
47	34.251379122025455	36.0	36.0	36.0	32.0	36.0
48	34.20681767976142	36.0	36.0	36.0	32.0	36.0
49	34.183335787833045	36.0	36.0	36.0	32.0	36.0
50	33.57872424309365	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	5.0
20	12.0
21	51.0
22	166.0
23	381.0
24	1011.0
25	2455.0
26	4774.0
27	8941.0
28	15102.0
29	23434.0
30	33541.0
31	48708.0
32	70689.0
33	113645.0
34	233782.0
35	747029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67132496996829	18.283290708502925	11.944106497128768	26.101277824400015
2	16.064685519853526	20.05004130478637	36.87017871127532	27.015094464084775
3	18.01986926660745	23.921280763843825	29.044612271634595	29.014237697914126
4	12.148623025661795	15.70166476442939	36.08912288452807	36.060589325380754
5	14.026238601916965	36.9643821410601	33.58998195942712	15.419397297595818
6	34.37419701240676	36.11091296093885	16.281654490018983	13.23323553663541
7	30.343315740181804	30.835903528882962	20.457887272412094	18.362893458523143
8	28.45639581262349	32.93969294208608	18.873645422971663	19.730265822318767
9	27.294141650916764	13.853980020127143	18.535458751626127	40.31641957732997
10	15.586794393577144	27.015637572206895	31.659583085005284	25.73798494921068
11	37.68592835315342	21.155179608016397	22.012106819827448	19.146785219002737
12	24.374446777927265	23.870353126347098	28.780510628767086	22.974689466958548
13	29.21822757546308	19.591598483413193	25.288923899895455	25.901250041228273
14	23.29766891381401	19.58554206517162	25.25475041937461	31.862038601639757
15	25.32912676989776	27.028013840851685	22.292485025048382	25.350374364202178
16	25.899176280814686	26.040847696708113	23.42372332730699	24.636252695170207
17	23.935744265675048	25.92128112612447	25.142667795736536	25.00030681246395
18	24.62344436134921	25.26660146886805	25.796161000210933	24.313793169571802
19	25.407370249009	25.127864094350972	25.272526171103177	24.19223948553686
20	25.7554931357855	24.5853014225469	24.995589537001102	24.6636159046665
21	26.72190825080078	24.067213406477425	24.771654823705557	24.439223519016238
22	25.796581646310923	24.33791737073789	25.011601355191694	24.853899627759493
23	24.637633358671067	24.07140791626429	25.599725708184494	25.691233016880144
24	24.703272630207973	25.270034562583128	25.121383239678398	24.9053095675305
25	24.97439984413628	24.604453621722126	25.33230344641379	25.088843087727803
26	24.716568942399444	25.165609649082437	25.672410460331925	24.445410948186193
27	25.185306212619462	24.95349606068486	24.894047725890246	24.96715000080543
28	24.529906924869486	24.761175689088873	25.682338737019418	25.02657864902223
29	24.585295060665892	24.959461470371206	25.467401192607493	24.987842276355412
30	24.485686671575845	25.15134081983309	25.777568114874818	24.58540439371625
31	24.997545319538027	24.985502043521485	24.714873522589198	25.30207911435129
32	24.67022426360888	24.84074039130498	24.76810036749562	25.720934977590517
33	24.512417943694686	24.43578859196826	25.399139971680125	25.65265349265693
34	25.077318638701048	24.664875860630087	25.63350658435709	24.62429891631178
35	25.417333682086195	24.539673067202173	25.425541135834894	24.61745211487674
36	24.629651221660033	25.054693181225435	25.07586473524818	25.23979086186635
37	25.51469685812469	24.909563696612665	24.700079774177713	24.87565967108493
38	24.707967941676433	24.8290151147877	25.369891932029482	25.093125011506384
39	25.24691642120766	24.763055351006383	24.94047619047619	25.04955203730977
40	25.216173365170945	24.99733450742848	25.284517361752982	24.501974765647592
41	24.403710399812226	25.390987253842372	25.61067188673355	24.594630459611857
42	25.64636740254152	25.226104905312457	25.037182377355684	24.09034531479034
43	24.94463880415215	24.499141297419445	25.385150543333605	25.171069355094804
44	24.67525394815262	24.945866070010915	24.936431463721636	25.44244851811483
45	24.76704906313377	25.1463517904316	25.085985512093202	25.000613634341434
46	24.73742384275115	25.14341686117463	24.926038396369766	25.193120899704464
47	24.699011432042855	24.99593466001485	25.381835140492015	24.92321876745028
48	25.452675244344146	25.422069902906664	24.592734184555578	24.532520668193612
49	24.753110141065672	25.56685911611321	24.800743104917547	24.879287637903566
50	24.563284779590553	25.573171947749117	24.95969195603317	24.903851316627165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	19.0
2	12.0
3	75.0
4	138.0
5	156.0
6	174.0
7	188.0
8	202.0
9	261.0
10	320.0
11	505.0
12	690.0
13	1255.5
14	1821.0
15	2522.0
16	3223.0
17	3504.5
18	3786.0
19	3930.5
20	4075.0
21	4679.5
22	5284.0
23	6025.5
24	6767.0
25	8024.0
26	9281.0
27	11352.0
28	13423.0
29	15265.5
30	17108.0
31	18772.0
32	20436.0
33	23048.5
34	25661.0
35	29275.5
36	32890.0
37	39805.5
38	46721.0
39	51656.5
40	56592.0
41	63323.0
42	70054.0
43	70929.5
44	71805.0
45	78443.0
46	85081.0
47	90173.0
48	95265.0
49	98463.0
50	101661.0
51	98612.5
52	95564.0
53	94949.0
54	94334.0
55	94227.5
56	94121.0
57	90420.0
58	86719.0
59	79613.5
60	72508.0
61	63871.0
62	55234.0
63	47989.5
64	40745.0
65	34738.5
66	28732.0
67	24508.0
68	20284.0
69	18636.0
70	16988.0
71	13424.0
72	9860.0
73	8209.5
74	6559.0
75	5001.0
76	3443.0
77	2975.0
78	2507.0
79	2048.0
80	1589.0
81	1255.0
82	921.0
83	744.5
84	568.0
85	399.0
86	230.0
87	170.0
88	110.0
89	96.5
90	83.0
91	63.0
92	43.0
93	33.5
94	24.0
95	25.5
96	27.0
97	23.5
98	20.0
99	19.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00813053029466269
2	3.835155799369193E-4
3	4.602186959243032E-4
4	0.0
5	0.0
6	2.301093479621516E-4
7	7.670311598738388E-5
8	0.0
9	0.0012272498557981421
10	6.903280438864548E-4
11	0.0
12	1.5340623197476777E-4
13	8.437342758612226E-4
14	7.670311598738388E-5
15	0.003911858915356578
16	5.369218119116871E-4
17	0.0
18	2.301093479621516E-4
19	0.0
20	7.670311598738386E-4
21	0.0
22	7.670311598738388E-5
23	5.369218119116871E-4
24	4.602186959243032E-4
25	0.0019175778996845967
26	0.0046021869592430325
27	0.005752733699053791
28	0.0038351557993691935
29	0.0023010934796215163
30	0.0036817495673944255
31	0.007363499134788851
32	0.002991421523507971
33	0.0038351557993691935
34	0.0024544997115962842
35	0.0026846090595584356
36	0.007056686670839317
37	0.0036817495673944255
38	0.007977124062687923
39	0.0036817495673944255
40	0.002224390363634132
41	0.003144827755482739
42	0.0014573592037602934
43	0.001303952971785526
44	0.001303952971785526
45	0.0015340623197476772
46	8.437342758612226E-4
47	0.0018408747836972127
48	0.002607905943571052
49	6.903280438864548E-4
50	0.0013806560877729097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1303728.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.97794791496526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97529466610935	44.428722781396466
2	13.387430116600168	15.255765917977637
3	3.9535660861415183	6.757982476036335
4	1.6454069037706516	3.7500763544787383
5	0.8559171906360267	2.438420255379146
6	0.5074971327489525	1.7349687118058412
7	0.31503691629578046	1.2565109905594574
8	0.2214567260480343	1.00945198417469
9	0.16415668140956557	0.8417979758922997
>10	0.8203214955193163	8.523368688027782
>50	0.07627344611186332	3.0147227405534447
>100	0.07019004652396771	7.936255042618592
>500	0.0066395820392605364	2.530429055351402
>1k	8.130100456237392E-4	0.5215270257482447
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3010934796215162E-4	0.0
10	0.0	0.0	0.0	2.3010934796215162E-4	0.0
11	0.0	0.0	0.0	2.3010934796215162E-4	0.0
12	0.0	7.670311598738387E-5	0.0	3.068124639495355E-4	7.670311598738387E-5
13	0.0	7.670311598738387E-5	0.0	5.369218119116871E-4	7.670311598738387E-5
14	0.0	1.5340623197476774E-4	0.0	5.369218119116871E-4	7.670311598738387E-5
15	0.0	1.5340623197476774E-4	0.0	5.369218119116871E-4	7.670311598738387E-5
16	0.0	1.5340623197476774E-4	0.0	5.369218119116871E-4	7.670311598738387E-5
17	0.0	2.3010934796215162E-4	0.0	5.369218119116871E-4	7.670311598738387E-5
18	0.0	2.3010934796215162E-4	0.0	5.369218119116871E-4	7.670311598738387E-5
19	0.0	2.3010934796215162E-4	0.0	6.903280438864548E-4	7.670311598738387E-5
20	0.0	2.3010934796215162E-4	0.0	7.670311598738387E-4	7.670311598738387E-5
21	0.0	2.3010934796215162E-4	0.0	0.0010738436238233741	7.670311598738387E-5
22	0.0	2.3010934796215162E-4	0.0	0.0016874685517224452	7.670311598738387E-5
23	0.0	2.3010934796215162E-4	0.0	0.0020709841316593645	7.670311598738387E-5
24	0.0	3.8351557993691936E-4	0.0	0.002607905943571052	7.670311598738387E-5
25	0.0	3.8351557993691936E-4	0.0	0.0029147184075205873	7.670311598738387E-5
26	0.0	3.8351557993691936E-4	0.0	0.003528343335419658	7.670311598738387E-5
27	0.0	3.8351557993691936E-4	0.0	0.004908999423192568	7.670311598738387E-5
28	0.0	3.8351557993691936E-4	0.0	0.009281077034473449	7.670311598738387E-5
29	0.0	3.8351557993691936E-4	0.0	0.016721279285249684	7.670311598738387E-5
30	0.0	3.8351557993691936E-4	0.0	0.029990918351067094	7.670311598738387E-5
31	0.0	3.8351557993691936E-4	0.0	0.05676030583066406	7.670311598738387E-5
32	0.0	3.8351557993691936E-4	0.0	0.08445013070210965	7.670311598738387E-5
33	0.0	3.8351557993691936E-4	0.0	0.10868831535412295	7.670311598738387E-5
34	0.0	3.8351557993691936E-4	0.0	0.13668495268951805	7.670311598738387E-5
35	0.0	3.8351557993691936E-4	0.0	0.1698206987960679	7.670311598738387E-5
36	0.0	3.8351557993691936E-4	0.0	0.2172999275922585	7.670311598738387E-5
37	0.0	3.8351557993691936E-4	0.0	0.2847986696611563	7.670311598738387E-5
38	0.0	4.6021869592430324E-4	0.0	0.3765355963820674	7.670311598738387E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2540	0.0	21.740005	1
AATACGA	330	0.0	21.333185	39
CGCAATA	330	0.0	20.668108	36
CAATACG	330	0.0	20.666523	38
CGAATGC	325	0.0	20.30677	43
ATACGAA	350	0.0	20.113373	40
GTACGGA	110	1.2659257E-7	19.999863	12
TACGGAC	115	2.0218431E-7	19.1303	13
CTAGCGG	335	0.0	19.045374	29
TACTATA	190	1.8189894E-12	18.525476	2
TAGACCG	60	0.007412706	18.333204	5
TTAGAAC	290	0.0	18.20677	3
TACGAAT	380	0.0	17.946556	41
GGTATCA	1275	0.0	17.772425	1
GTATTAG	360	0.0	17.7221	1
TCTAGCG	365	0.0	17.480001	28
ATTACAC	220	1.8189894E-12	16.999882	3
GGCGCAA	600	0.0	16.867844	34
CGGCGCA	620	0.0	16.678583	33
TAGCGGC	390	0.0	16.360743	30
>>END_MODULE