FastQCFastQC Report
Thu 2 Feb 2017
SRR4064239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246755
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41780.33510994541830597No Hit
TATCAACGCAGAGTACTTTTTTTTT27620.22153510513292507No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22880.18351640859671706No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16070.12889461040862077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14410.11558004579889393No Hit
GGTATCAACGCAGAGTACTTTTTTT13970.1120508840951109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA1100.018.13586413
GATAACG1300.016.07777211
CGGACAT851.020453E-915.6447445
ATAACGA1400.015.60671412
ACGAACG1300.015.34758215
CGATAAC1400.014.92995910
AACGAAC1350.014.77855814
CGAACGA1400.014.25132516
CCGATAA1500.013.934639
GTTAATT1550.013.4791722
TTAATAC1205.2750693E-1113.4541843
ATTCCGA1700.013.4113966
AATTCCG1700.013.40978055
CCCTATA500.001498659313.3007031
CGAGACT802.0045245E-613.0589954
CGACCAT1850.012.83903210
ATCCTAG604.089189E-412.6673361
TAATTCC2250.012.6632684
GGTTAAT2100.012.2149311
TTCCGAT1950.012.180627
Produced by FastQC (version 0.11.5)