##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064239_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246755
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156063540952314	32.0	32.0	32.0	32.0	32.0
2	30.741221811823493	32.0	32.0	32.0	32.0	32.0
3	30.76194841809337	32.0	32.0	32.0	32.0	32.0
4	30.792644906176434	32.0	32.0	32.0	32.0	32.0
5	30.7107731671419	32.0	32.0	32.0	32.0	32.0
6	34.346315033827814	36.0	36.0	36.0	32.0	36.0
7	34.26354496272323	36.0	36.0	36.0	32.0	36.0
8	34.23690941684613	36.0	36.0	36.0	32.0	36.0
9	34.360726846894536	36.0	36.0	36.0	32.0	36.0
10	34.05259573853724	36.0	36.0	36.0	32.0	36.0
11	34.34025690693039	36.0	36.0	36.0	32.0	36.0
12	34.1527084310871	36.0	36.0	36.0	32.0	36.0
13	34.23943437162875	36.0	36.0	36.0	32.0	36.0
14	34.13367502035283	36.0	36.0	36.0	32.0	36.0
15	34.07505644653521	36.0	36.0	36.0	32.0	36.0
16	34.0740025105173	36.0	36.0	36.0	32.0	36.0
17	34.004852597342705	36.0	36.0	36.0	32.0	36.0
18	33.990067013968265	36.0	36.0	36.0	32.0	36.0
19	34.01026264181816	36.0	36.0	36.0	32.0	36.0
20	33.98264695148606	36.0	36.0	36.0	32.0	36.0
21	33.962708792024095	36.0	36.0	36.0	32.0	36.0
22	33.949579508403815	36.0	36.0	36.0	32.0	36.0
23	33.898855027651784	36.0	36.0	36.0	32.0	36.0
24	33.88580916058087	36.0	36.0	36.0	32.0	36.0
25	33.497620623137664	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	8.0
5	12.0
6	44.0
7	16.0
8	83.0
9	73.0
10	114.0
11	22.0
12	87.0
13	68.0
14	163.0
15	259.0
16	389.0
17	530.0
18	756.0
19	1004.0
20	1599.0
21	2490.0
22	3800.0
23	5825.0
24	8299.0
25	11872.0
26	16407.0
27	20842.0
28	27707.0
29	36428.0
30	46126.0
31	61237.0
32	84181.0
33	115970.0
34	246421.0
35	553921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.338823929670724	17.487394202777608	11.386461165157609	25.787320702394055
2	16.842974092915135	20.259632337252523	37.0204686665287	25.87692490330364
3	18.786951280255835	24.395083830428565	28.55961493266856	28.258349956647038
4	12.92893724708534	15.514371769008955	35.73551292797472	35.821178055930986
5	14.83163318100546	36.32767097179407	33.287530692181384	15.55316515501908
6	33.66126793893742	35.46555002045581	17.028212964968432	13.84496907563834
7	29.688072864561484	30.58106968680529	21.254463580154308	18.476393868478922
8	27.96388092322851	32.29911692163021	20.04281397584252	19.69418817929877
9	27.555347984730705	14.23997380755777	18.934632041927166	39.27004616578436
10	16.325318609354436	26.471060319090878	31.247552245513788	25.956068826040894
11	36.695691253300375	21.583779402441277	22.54937523574117	19.17115410851718
12	24.76156920091271	23.755998391600368	28.793965130473797	22.688467277013128
13	29.249954654095017	19.665034214087246	25.505667034249402	25.579344097568335
14	23.80336663367934	19.595997537588296	25.37191004652738	31.22872578220498
15	25.07855029265251	26.834144317453134	22.622243516741747	25.46506187315261
16	25.570311916856415	25.903794802482622	23.659226239444724	24.866667041216235
17	23.732291894902684	25.75746266560404	25.426635815176784	25.083609624316495
18	24.553061145858752	25.061165123357693	26.255089066694065	24.13068466408949
19	25.346247603055566	24.933840721687965	25.819817202134754	23.90009447312171
20	25.69729174359057	24.336501260214156	25.069994060137095	24.89621293605818
21	26.8388396583831	24.230639568483912	24.65268413279394	24.27783664033905
22	25.797852486689415	24.20050200150424	25.226659217715657	24.774986294090688
23	24.55147357487	24.153077754213136	25.67393353059131	25.621515140325556
24	24.64460758737717	25.01633480654291	25.417601135329253	24.921456470750663
25	24.76780185758514	24.558701119190633	25.498008927517894	25.175488095706335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	204.0
2	453.0
3	702.0
4	702.0
5	702.0
6	1926.0
7	3150.0
8	3150.0
9	3150.0
10	3288.0
11	3426.0
12	3426.0
13	3426.0
14	3572.5
15	3719.0
16	3719.0
17	3719.0
18	6228.0
19	8737.0
20	8737.0
21	8737.0
22	13792.5
23	18848.0
24	18848.0
25	18848.0
26	27461.0
27	36074.0
28	36074.0
29	36074.0
30	44708.5
31	53343.0
32	53343.0
33	53343.0
34	65306.5
35	77270.0
36	77270.0
37	77270.0
38	88688.5
39	100107.0
40	100107.0
41	100107.0
42	115375.5
43	130644.0
44	130644.0
45	130644.0
46	145831.5
47	161019.0
48	161019.0
49	161019.0
50	165736.0
51	170453.0
52	170453.0
53	170453.0
54	158600.5
55	146748.0
56	146748.0
57	146748.0
58	134279.0
59	121810.0
60	121810.0
61	121810.0
62	106637.5
63	91465.0
64	91465.0
65	91465.0
66	74611.5
67	57758.0
68	57758.0
69	57758.0
70	43654.0
71	29550.0
72	29550.0
73	29550.0
74	22362.5
75	15175.0
76	15175.0
77	15175.0
78	12240.5
79	9306.0
80	9306.0
81	9306.0
82	6403.0
83	3500.0
84	3500.0
85	3500.0
86	2674.5
87	1849.0
88	1849.0
89	1849.0
90	1354.5
91	860.0
92	860.0
93	860.0
94	587.0
95	314.0
96	314.0
97	314.0
98	519.0
99	724.0
100	724.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04034473493188317
2	0.0055343672172961005
3	9.624986464862785E-4
4	0.003208328821620928
5	0.008181238495133367
6	0.013234356389186328
7	0.02229788531026545
8	0.03473015949404654
9	0.04764368300107078
10	0.057348877686474085
11	0.05494263107025839
12	0.06384574355025646
13	0.06232178735998652
14	0.06593115728431007
15	0.059193666758906124
16	0.06528949151998588
17	0.06304366134485123
18	0.07595718485187547
19	0.07202698204538983
20	0.0749946862053892
21	0.07339052179457872
22	0.07555614374917285
23	0.08093009452538791
24	0.07611760129295651
25	0.0757165601902539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1246755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.71289316750172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04139958634937	41.91829358623897
2	12.90584565261979	13.864206175508667
3	3.836618386275662	6.182276205194925
4	1.6548572350414232	3.55548679492989
5	0.9162208648962861	2.4606436717005122
6	0.5533203286003245	1.7832261418509707
7	0.3872321507092635	1.4559551399448019
8	0.27950464077432696	1.2010402327785896
9	0.21373620653743997	1.0332351024995337
>10	1.0396690334288503	9.998752680003221
>50	0.08327874283195086	3.1378303820286217
>100	0.07855422516471826	8.770810571614234
>500	0.007359759873197039	2.6564293755007156
>1k	0.002403186897370462	1.981813940206368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4178	0.33510994541830597	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2762	0.22153510513292507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2288	0.18351640859671706	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1607	0.12889461040862077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1441	0.11558004579889393	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1397	0.1120508840951109	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.02082205405232E-5	0.0	0.0	0.0
6	0.0	8.02082205405232E-5	0.0	0.0	0.0
7	0.0	8.02082205405232E-5	0.0	0.0	0.0
8	0.0	8.02082205405232E-5	0.0	0.0	0.0
9	0.0	8.02082205405232E-5	0.0	0.0	0.0
10	0.0	8.02082205405232E-5	0.0	0.0	0.0
11	0.0	8.02082205405232E-5	0.0	0.0	0.0
12	0.0	8.02082205405232E-5	0.0	0.0	8.02082205405232E-5
13	0.0	8.02082205405232E-5	0.0	0.0	8.02082205405232E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	110	0.0	18.135864	13
GATAACG	130	0.0	16.077772	11
CGGACAT	85	1.020453E-9	15.644744	5
ATAACGA	140	0.0	15.606714	12
ACGAACG	130	0.0	15.347582	15
CGATAAC	140	0.0	14.929959	10
AACGAAC	135	0.0	14.778558	14
CGAACGA	140	0.0	14.251325	16
CCGATAA	150	0.0	13.93463	9
GTTAATT	155	0.0	13.479172	2
TTAATAC	120	5.2750693E-11	13.454184	3
ATTCCGA	170	0.0	13.411396	6
AATTCCG	170	0.0	13.4097805	5
CCCTATA	50	0.0014986593	13.300703	1
CGAGACT	80	2.0045245E-6	13.058995	4
CGACCAT	185	0.0	12.839032	10
ATCCTAG	60	4.089189E-4	12.667336	1
TAATTCC	225	0.0	12.663268	4
GGTTAAT	210	0.0	12.214931	1
TTCCGAT	195	0.0	12.18062	7
>>END_MODULE