FastQCFastQC Report
Thu 2 Feb 2017
SRR4064239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246755
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.10354881271781545No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12590.1009821496605187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC450.001397547824.44651823
CGTCTTA651.8145127E-523.69051415
GTATCAA25850.020.4256251
AGGTACG550.00448148920.00169622
GCGGATA550.00448669319.9976842
CTATTAG808.988545E-519.2500881
CGCAATA3550.019.21366736
TAGCGGC3900.018.61696430
CTAGCGG3700.018.43325629
TCTAGCG3900.017.4879628
TACGAAT3900.017.48655741
GCCGGTC4450.017.3020618
GTATTAT1307.316903E-716.9231531
ATACGAA4050.016.8389140
CGCCGGT4450.016.8077167
AACGCAG31400.016.7439566
TAACGCC3550.016.7311294
CGAATGC4000.016.4987543
TGGTCGG3800.016.21255137
CGGTCCA4350.016.18333210
Produced by FastQC (version 0.11.5)