##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246755
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27746429731583	32.0	32.0	32.0	32.0	32.0
2	31.396017661850163	32.0	32.0	32.0	32.0	32.0
3	31.465080950146582	32.0	32.0	32.0	32.0	32.0
4	31.542846830371644	32.0	32.0	32.0	32.0	32.0
5	31.499797474243135	32.0	32.0	32.0	32.0	32.0
6	35.06175792356959	36.0	36.0	36.0	36.0	36.0
7	35.07338089680811	36.0	36.0	36.0	36.0	36.0
8	35.02915649024869	36.0	36.0	36.0	36.0	36.0
9	35.119917706365726	36.0	36.0	36.0	36.0	36.0
10	34.987300632441816	36.0	36.0	36.0	36.0	36.0
11	35.121508235379046	36.0	36.0	36.0	36.0	36.0
12	35.02891987599809	36.0	36.0	36.0	36.0	36.0
13	35.067281863718215	36.0	36.0	36.0	36.0	36.0
14	35.008891081246915	36.0	36.0	36.0	36.0	36.0
15	34.991918219698334	36.0	36.0	36.0	36.0	36.0
16	35.00014678104359	36.0	36.0	36.0	36.0	36.0
17	34.96297027082306	36.0	36.0	36.0	36.0	36.0
18	34.95649666534323	36.0	36.0	36.0	36.0	36.0
19	34.951353714242174	36.0	36.0	36.0	36.0	36.0
20	34.94863064515482	36.0	36.0	36.0	36.0	36.0
21	34.94647865859772	36.0	36.0	36.0	36.0	36.0
22	34.928836058407626	36.0	36.0	36.0	36.0	36.0
23	34.88033494952898	36.0	36.0	36.0	32.0	36.0
24	34.84821556761352	36.0	36.0	36.0	32.0	36.0
25	34.82144928233695	36.0	36.0	36.0	32.0	36.0
26	34.75726666426042	36.0	36.0	36.0	32.0	36.0
27	34.75789148629843	36.0	36.0	36.0	32.0	36.0
28	34.736591391251686	36.0	36.0	36.0	32.0	36.0
29	34.70378221863959	36.0	36.0	36.0	32.0	36.0
30	34.68800044916603	36.0	36.0	36.0	32.0	36.0
31	34.678308889878124	36.0	36.0	36.0	32.0	36.0
32	34.62975083316289	36.0	36.0	36.0	32.0	36.0
33	34.60897529987849	36.0	36.0	36.0	32.0	36.0
34	34.5820590252295	36.0	36.0	36.0	32.0	36.0
35	34.53907704400624	36.0	36.0	36.0	32.0	36.0
36	34.51680562740875	36.0	36.0	36.0	32.0	36.0
37	34.50616039237862	36.0	36.0	36.0	32.0	36.0
38	34.47067306728267	36.0	36.0	36.0	32.0	36.0
39	34.46541622050844	36.0	36.0	36.0	32.0	36.0
40	34.4350790652534	36.0	36.0	36.0	32.0	36.0
41	34.410354079189574	36.0	36.0	36.0	32.0	36.0
42	34.34032267767123	36.0	36.0	36.0	32.0	36.0
43	34.31767107410838	36.0	36.0	36.0	32.0	36.0
44	34.25922976045815	36.0	36.0	36.0	32.0	36.0
45	34.22151505307779	36.0	36.0	36.0	32.0	36.0
46	34.18406423074301	36.0	36.0	36.0	32.0	36.0
47	34.15584457251023	36.0	36.0	36.0	32.0	36.0
48	34.09941969352439	36.0	36.0	36.0	32.0	36.0
49	34.06167611118464	36.0	36.0	36.0	32.0	36.0
50	33.48168084346965	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	15.0
21	66.0
22	196.0
23	455.0
24	1255.0
25	2822.0
26	5695.0
27	10157.0
28	16557.0
29	24447.0
30	33848.0
31	46639.0
32	66675.0
33	102026.0
34	206848.0
35	729048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29198545230528	18.45654541983447	11.956125898201432	26.29534322965882
2	16.03152195708843	21.034850611590134	36.49440545418087	26.439221977140566
3	18.099954361302874	24.935572436793613	28.86122226687168	28.10325093503183
4	12.512241779659997	16.046617017778154	35.92662551984953	35.51451568271232
5	14.36737771254176	36.66566406390991	33.2147053751539	15.752252848394432
6	33.76945251428911	35.760462769720405	16.791042980411532	13.67904173557895
7	29.499644677298008	30.74447725854499	21.324415241499125	18.431462822657878
8	27.990743971349623	32.53670528692486	19.84960958648652	19.622941155239
9	27.53095084360349	14.048374353812157	18.82862035637084	39.59205444621351
10	16.12720815179379	26.723770076294734	31.35094293616011	25.798078835751365
11	36.97249016245386	21.42218914076073	22.521042643536738	19.084278053248678
12	24.717486142002464	23.85773284684296	28.757500483255306	22.667280527899273
13	29.273437775717305	19.531693654210795	25.6771215995534	25.517746970518502
14	23.883158893541864	19.441701764503474	25.46302274788992	31.212116594064742
15	25.116448822276677	26.894283640213846	22.628811608385437	25.360455929124036
16	25.645319896755232	25.82931888291601	23.65798647037969	24.867374749949068
17	23.778147092369466	25.809181281952974	25.447923166879754	24.964748458797807
18	24.707740358740438	24.954221011252447	26.302445315864997	24.03559331414212
19	25.415578842675586	24.75402143965735	25.975111389166276	23.855288328500787
20	25.766334308404193	24.165528371032778	25.304331993580092	24.76380532698294
21	26.82082686654555	24.18342015873207	24.82115572024977	24.17459725447261
22	25.82562398035218	24.026792775479365	25.46805544638317	24.67952779778529
23	24.50537278212821	23.948964785958978	25.913196502578312	25.6324659293345
24	24.71710401781274	24.79209912508382	25.665250716263433	24.82554614084001
25	24.91824659090089	24.230130332219225	25.78442766998974	25.067195406890146
26	24.74741073949537	25.000481275186736	26.01123937986088	24.24086860545702
27	25.00557479774313	24.66715650021417	25.46286863851407	24.86440006352863
28	24.48739122378803	24.55974159207707	26.20839954087194	24.744467643262954
29	24.372114437517848	24.69287508702808	25.95177441037471	24.98323606507936
30	24.437056079826423	24.938297351819397	26.066150372381596	24.558496195972584
31	24.994344898617268	24.827178497459617	25.081136667356507	25.09733993656661
32	24.664619155750664	24.912790954162013	25.16288780854035	25.259702081546976
33	24.47208715474909	24.559758466738643	25.553339932076792	25.414814446435475
34	25.14810839946676	24.442899058490987	25.784818107003804	24.624174435038444
35	25.653855192942128	24.467783890702087	25.612466622443996	24.26589429391179
36	24.639755120113712	25.240083038410077	25.155616804047966	24.96454503742825
37	25.340236864374493	24.891854929594412	25.388925206845244	24.37898299918585
38	24.654472881636007	24.832711130505373	25.68227066865814	24.83054531920048
39	25.040747442857324	24.619877693502225	25.14470154904452	25.194673314595928
40	25.29274605788427	24.98257440069173	25.394933461883056	24.329746079540943
41	24.389136605762808	25.27089103478811	25.76651194054153	24.573460418907548
42	25.40281440699174	25.397360129875963	25.257153124017428	23.942672339114868
43	24.824422092818068	24.52957312671447	25.487030174695608	25.158974605771856
44	24.572427284765872	25.003629445268366	25.151373935529826	25.272569334435936
45	24.75341710908681	25.131522906521887	25.23459162729067	24.880468357100632
46	24.54806351895254	25.128534601274037	25.127171051681742	25.196230828091686
47	24.667249524756762	25.02330095529906	25.507206853127784	24.80224266681639
48	25.46142302210323	25.5382633602328	24.571181985673046	24.429131631990927
49	24.563505813505813	25.58728600395267	24.834530042863374	25.014678139678136
50	24.74024213587779	25.687754764834313	24.63260123618595	24.93940186310195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	47.0
2	41.0
3	122.0
4	203.0
5	259.5
6	316.0
7	329.5
8	343.0
9	489.5
10	636.0
11	884.5
12	1133.0
13	2081.5
14	3030.0
15	3967.0
16	4904.0
17	5119.0
18	5334.0
19	5158.0
20	4982.0
21	5378.0
22	5774.0
23	6257.5
24	6741.0
25	7522.5
26	8304.0
27	10013.5
28	11723.0
29	13404.0
30	15085.0
31	16810.5
32	18536.0
33	21309.5
34	24083.0
35	28098.5
36	32114.0
37	38238.0
38	44362.0
39	49107.5
40	53853.0
41	58774.5
42	63696.0
43	65928.5
44	68161.0
45	74611.0
46	81061.0
47	86253.0
48	91445.0
49	94295.0
50	97145.0
51	94919.5
52	92694.0
53	91693.0
54	90692.0
55	91274.0
56	91856.0
57	88551.0
58	85246.0
59	77979.5
60	70713.0
61	61649.0
62	52585.0
63	45503.0
64	38421.0
65	32304.0
66	26187.0
67	22235.5
68	18284.0
69	16261.5
70	14239.0
71	11053.5
72	7868.0
73	6784.0
74	5700.0
75	4423.5
76	3147.0
77	2742.5
78	2338.0
79	1977.0
80	1616.0
81	1278.0
82	940.0
83	785.5
84	631.0
85	449.5
86	268.0
87	185.5
88	103.0
89	79.5
90	56.0
91	50.0
92	44.0
93	33.0
94	22.0
95	21.0
96	20.0
97	17.0
98	14.0
99	13.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00778019739243075
2	4.01041102702616E-4
3	4.8124932324313923E-4
4	0.0
5	0.0
6	8.02082205405232E-5
7	8.02082205405232E-5
8	0.0
9	0.001604164410810464
10	8.822904259457551E-4
11	8.02082205405232E-5
12	1.604164410810464E-4
13	5.614575437836624E-4
14	1.604164410810464E-4
15	0.005614575437836624
16	7.218739848647088E-4
17	8.02082205405232E-5
18	3.208328821620928E-4
19	0.0
20	6.416657643241856E-4
21	0.0
22	8.02082205405232E-5
23	6.416657643241856E-4
24	5.614575437836624E-4
25	0.0014437479697294176
26	0.005373950776215055
27	0.005855200099458194
28	0.0035291617037830204
29	0.0024864548367562194
30	0.00401041102702616
31	0.007459364510268657
32	0.0030479123805398816
33	0.00425103568864773
34	0.0026468712778372656
35	0.002727079498377789
36	0.008261446715673889
37	0.00401041102702616
38	0.008742696038917029
39	0.0040906192475666835
40	0.0020854137340536034
41	0.0028874959394588353
42	0.0021656219545941264
43	0.001203123308107848
44	6.416657643241856E-4
45	0.0011229150875673249
46	7.218739848647088E-4
47	0.00200520551351308
48	0.0011229150875673249
49	2.4062466162156962E-4
50	0.0013635397491888945
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1246755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84874838413112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95335936061028	44.88399660373217
2	12.594249720317677	14.319346668745068
3	3.657518181892652	6.237759924984003
4	1.5464042969231933	3.516445951037032
5	0.8344300658428165	2.371815242862612
6	0.5189324412556457	1.7700395868783063
7	0.3505613664355197	1.3950282439583033
8	0.256840876544718	1.1680865892360286
9	0.19130531628234665	0.9787921010893571
>10	0.9500784271611149	9.587205833587154
>50	0.07177943330655333	2.861815104431334
>100	0.06715746092892769	7.751699087991404
>500	0.006247198267937493	2.4233830824662057
>1k	0.0011358542305340898	0.7345859790009637
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.10354881271781545	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1259	0.1009821496605187	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.02082205405232E-5
2	8.02082205405232E-5	0.0	0.0	0.0	8.02082205405232E-5
3	8.02082205405232E-5	0.0	0.0	0.0	8.02082205405232E-5
4	8.02082205405232E-5	0.0	0.0	0.0	1.604164410810464E-4
5	8.02082205405232E-5	0.0	0.0	0.0	1.604164410810464E-4
6	8.02082205405232E-5	0.0	0.0	0.0	3.208328821620928E-4
7	8.02082205405232E-5	0.0	0.0	0.0	3.208328821620928E-4
8	8.02082205405232E-5	0.0	0.0	0.0	3.208328821620928E-4
9	8.02082205405232E-5	0.0	0.0	0.0	4.01041102702616E-4
10	8.02082205405232E-5	0.0	0.0	0.0	4.01041102702616E-4
11	8.02082205405232E-5	0.0	0.0	0.0	4.01041102702616E-4
12	8.02082205405232E-5	0.0	0.0	0.0	4.812493232431392E-4
13	8.02082205405232E-5	0.0	0.0	0.0	4.812493232431392E-4
14	2.406246616215696E-4	0.0	0.0	0.0	4.812493232431392E-4
15	2.406246616215696E-4	0.0	0.0	0.0	4.812493232431392E-4
16	2.406246616215696E-4	0.0	0.0	0.0	4.812493232431392E-4
17	2.406246616215696E-4	0.0	0.0	8.02082205405232E-5	5.614575437836624E-4
18	2.406246616215696E-4	0.0	0.0	8.02082205405232E-5	5.614575437836624E-4
19	2.406246616215696E-4	0.0	0.0	8.02082205405232E-5	5.614575437836624E-4
20	2.406246616215696E-4	0.0	0.0	8.02082205405232E-5	6.416657643241856E-4
21	2.406246616215696E-4	0.0	0.0	8.02082205405232E-5	6.416657643241856E-4
22	2.406246616215696E-4	0.0	0.0	1.604164410810464E-4	6.416657643241856E-4
23	2.406246616215696E-4	0.0	0.0	4.812493232431392E-4	7.218739848647088E-4
24	2.406246616215696E-4	0.0	0.0	7.218739848647088E-4	7.218739848647088E-4
25	2.406246616215696E-4	0.0	0.0	9.624986464862784E-4	7.218739848647088E-4
26	2.406246616215696E-4	0.0	0.0	0.001203123308107848	8.02082205405232E-4
27	2.406246616215696E-4	0.0	0.0	0.0018447890724320336	8.02082205405232E-4
28	3.208328821620928E-4	0.0	0.0	0.005774991878917671	8.02082205405232E-4
29	3.208328821620928E-4	0.0	0.0	0.012191649522159526	8.02082205405232E-4
30	3.208328821620928E-4	0.0	0.0	0.024142674382697484	8.02082205405232E-4
31	3.208328821620928E-4	0.0	0.0	0.05021034605836752	8.02082205405232E-4
32	3.208328821620928E-4	0.0	0.0	0.07828322324755065	8.02082205405232E-4
33	3.208328821620928E-4	0.0	0.0	0.1030675633945723	8.02082205405232E-4
34	3.208328821620928E-4	0.0	0.0	0.1286539857469992	8.02082205405232E-4
35	3.208328821620928E-4	0.0	0.0	0.16065706574266797	8.02082205405232E-4
36	3.208328821620928E-4	0.0	0.0	0.20942366383130606	8.02082205405232E-4
37	3.208328821620928E-4	0.0	0.0	0.2776006512907508	8.02082205405232E-4
38	3.208328821620928E-4	0.0	0.0	0.3711234364410008	8.02082205405232E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	45	0.0013975478	24.446518	23
CGTCTTA	65	1.8145127E-5	23.690514	15
GTATCAA	2585	0.0	20.425625	1
AGGTACG	55	0.004481489	20.001696	22
GCGGATA	55	0.004486693	19.997684	2
CTATTAG	80	8.988545E-5	19.250088	1
CGCAATA	355	0.0	19.213667	36
TAGCGGC	390	0.0	18.616964	30
CTAGCGG	370	0.0	18.433256	29
TCTAGCG	390	0.0	17.48796	28
TACGAAT	390	0.0	17.486557	41
GCCGGTC	445	0.0	17.302061	8
GTATTAT	130	7.316903E-7	16.923153	1
ATACGAA	405	0.0	16.83891	40
CGCCGGT	445	0.0	16.807716	7
AACGCAG	3140	0.0	16.743956	6
TAACGCC	355	0.0	16.731129	4
CGAATGC	400	0.0	16.49875	43
TGGTCGG	380	0.0	16.212551	37
CGGTCCA	435	0.0	16.183332	10
>>END_MODULE