FastQCFastQC Report
Thu 2 Feb 2017
SRR4064238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094796
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37380.3414334725373494No Hit
TATCAACGCAGAGTACTTTTTTTTT25670.23447290636794435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19340.17665391543264683No Hit
GATTAAGAGGGACGGCCGGGGGCAT14310.13070928282529348No Hit
GCGCAAGACGGACCAGAGCGAAAGC14220.12988721186412813No Hit
GTATCTGATCGTCTTCGAACCTCCG13750.12559417462248676No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13420.12257991443154706No Hit
ATCAGATACCGTCGTAGTTCCGACC13410.12248857321363979No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13400.12239723199573255No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13320.12166650225247443No Hit
GAATAGGACCGCGGTTCTATTTTGT13200.12057040763758728No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13010.11883492449734928No Hit
GGTATCAACGCAGAGTACTTTTTTT12990.11865224206153474No Hit
GAACTACGACGGTATCTGATCGTCT12970.11846955962572021No Hit
CTATTGGAGCTGGAATTACCGCGGC12940.11819553597199844No Hit
GTCCTATTCCATTATTCCTAGCTGC12600.11508993456315149No Hit
TCGTAGTTCCGACCATAAACGATGC12230.11171030950058276No Hit
GTACATGGGGAATAATTGCAATCCC12180.11125360341104644No Hit
GTACATGGGGTGGTATCAACGCAAA11980.10942677905290117No Hit
GGGTAGGCACACGCTGAGCCAGTCA11460.1046770357217235No Hit
GAATAACGCCGCCGCATCGCCAGTC11280.10303289379939276No Hit
CTCTTAATCATGGCCTCAGTTCCGA11080.10120606944124749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA508.709114E-515.200221
TACCTAT508.709114E-515.200226
CGGGTAA602.5643296E-514.25150919
CCGTCCG400.00527567914.2508569
GTATAGA602.5665408E-514.2502061
ACGAACG1301.8189894E-1213.88608515
CGCAAGG551.95756E-413.81964516
GCGTTAT1600.013.6564461
CCGTCGT2050.013.4398339
CGAACGA1351.8189894E-1213.37178616
CGGACAT1650.013.2395915
CGAGCCG2300.013.2187915
CCGGTTT655.4442036E-513.15523812
TCCAACG1102.737579E-912.95591618
GATAACG1403.6379788E-1212.89363211
CGATAAC1403.6379788E-1212.89363210
CGTTATT1700.012.8496042
ACCGTCG2300.012.8045338
TGCGTAC604.0882317E-412.66742911
TAACGAA1501.8189894E-1212.66742713
Produced by FastQC (version 0.11.5)