##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064238_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1094796
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200892221016517	32.0	32.0	32.0	32.0	32.0
2	30.782192298839234	32.0	32.0	32.0	32.0	32.0
3	30.809577309380014	32.0	32.0	32.0	32.0	32.0
4	30.84073379880818	32.0	32.0	32.0	32.0	32.0
5	30.752095367538793	32.0	32.0	32.0	32.0	32.0
6	34.396555157307844	36.0	36.0	36.0	32.0	36.0
7	34.30977734664723	36.0	36.0	36.0	32.0	36.0
8	34.27150994340498	36.0	36.0	36.0	32.0	36.0
9	34.41269423709988	36.0	36.0	36.0	32.0	36.0
10	34.107906861186926	36.0	36.0	36.0	32.0	36.0
11	34.400369566567655	36.0	36.0	36.0	32.0	36.0
12	34.18979883010168	36.0	36.0	36.0	32.0	36.0
13	34.29364557415263	36.0	36.0	36.0	32.0	36.0
14	34.176241966539884	36.0	36.0	36.0	32.0	36.0
15	34.111991640451734	36.0	36.0	36.0	32.0	36.0
16	34.10853620217831	36.0	36.0	36.0	32.0	36.0
17	34.02733659969529	36.0	36.0	36.0	32.0	36.0
18	34.029093091315644	36.0	36.0	36.0	32.0	36.0
19	34.038470180746	36.0	36.0	36.0	32.0	36.0
20	34.023348642121455	36.0	36.0	36.0	32.0	36.0
21	33.9976954610722	36.0	36.0	36.0	32.0	36.0
22	33.98002184881933	36.0	36.0	36.0	32.0	36.0
23	33.93466819389183	36.0	36.0	36.0	32.0	36.0
24	33.89904603232018	36.0	36.0	36.0	32.0	36.0
25	33.516027643506185	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	4.0
5	19.0
6	47.0
7	20.0
8	55.0
9	65.0
10	104.0
11	36.0
12	69.0
13	52.0
14	114.0
15	175.0
16	338.0
17	462.0
18	594.0
19	781.0
20	1355.0
21	1855.0
22	2851.0
23	4507.0
24	6448.0
25	9370.0
26	13148.0
27	17138.0
28	23335.0
29	31367.0
30	40580.0
31	54890.0
32	76557.0
33	107498.0
34	225771.0
35	475186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.266347343471026	17.420718970612153	11.614513184417369	25.698420501499452
2	16.708869084810416	20.148640205202405	37.297711569308795	25.84477914067838
3	18.733352819744606	24.38891832148925	28.57469080545863	28.303038053307517
4	12.482313555877289	15.610016761588103	35.967445068119645	35.940224614414966
5	14.655954398257048	36.55547892335308	33.449408282672344	15.339158395717527
6	33.673046179144016	35.881697346183714	16.98515507239757	13.460101402274699
7	29.8036188222611	30.627972098249973	21.113968690472383	18.45444038901654
8	28.099341197540227	32.39197375754973	19.787191272009576	19.721493772900466
9	27.556704987845666	14.035603969806079	18.757882039003526	39.64980900334473
10	16.216233506889704	26.356623154192814	31.194906819696044	26.232236519221434
11	37.33004566844363	21.11541769215795	22.541768301877827	19.012768337520598
12	24.924250802735838	23.828526667830516	28.796028776925812	22.451193752507837
13	29.41151739051752	19.336222765336323	25.48016546903305	25.77209437511311
14	23.83570483834553	19.988738893139228	25.121591618519034	31.053964649996207
15	25.074374228267416	27.248249520851747	22.543685433801684	25.133690817079156
16	25.647182487089253	25.584205475069695	24.037475435309172	24.731136602531876
17	23.766883770923627	25.670369564700785	25.308884578895125	25.25386208548046
18	24.80933510674766	24.6779744683185	26.505048288975118	24.00764213595872
19	25.572271370251944	24.529559203417996	25.517516869687945	24.38065255664211
20	25.862426742747864	24.054431818077937	24.995543545728705	25.08759789344549
21	27.384415123798174	23.950919505136394	24.413003058618337	24.251662312447095
22	26.122830303894574	24.059722176515542	25.06673175297916	24.750715766610725
23	24.42523665650411	23.927012437322023	25.685881056966682	25.96186984920718
24	24.722422824129513	24.884224767581106	25.518908888137265	24.874443520152113
25	24.851958499017325	24.420585949997715	25.526486585310117	25.200968965674846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	389.0
1	389.0
2	507.5
3	626.0
4	626.0
5	626.0
6	1648.5
7	2671.0
8	2671.0
9	2671.0
10	2662.5
11	2654.0
12	2654.0
13	2654.0
14	2783.5
15	2913.0
16	2913.0
17	2913.0
18	5111.0
19	7309.0
20	7309.0
21	7309.0
22	12010.5
23	16712.0
24	16712.0
25	16712.0
26	25283.0
27	33854.0
28	33854.0
29	33854.0
30	42328.5
31	50803.0
32	50803.0
33	50803.0
34	60616.0
35	70429.0
36	70429.0
37	70429.0
38	78676.5
39	86924.0
40	86924.0
41	86924.0
42	99443.5
43	111963.0
44	111963.0
45	111963.0
46	124747.0
47	137531.0
48	137531.0
49	137531.0
50	141423.0
51	145315.0
52	145315.0
53	145315.0
54	134333.0
55	123351.0
56	123351.0
57	123351.0
58	114407.0
59	105463.0
60	105463.0
61	105463.0
62	93838.0
63	82213.0
64	82213.0
65	82213.0
66	68371.5
67	54530.0
68	54530.0
69	54530.0
70	40956.0
71	27382.0
72	27382.0
73	27382.0
74	21097.0
75	14812.0
76	14812.0
77	14812.0
78	12125.0
79	9438.0
80	9438.0
81	9438.0
82	6485.0
83	3532.0
84	3532.0
85	3532.0
86	2806.0
87	2080.0
88	2080.0
89	2080.0
90	1462.5
91	845.0
92	845.0
93	845.0
94	597.0
95	349.0
96	349.0
97	349.0
98	528.5
99	708.0
100	708.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03635380472709071
2	0.006576567689322942
3	0.0014614594865162095
4	0.0028315777551251556
5	0.009225463008633571
6	0.01333581781446041
7	0.022013233515650404
8	0.03525771011220355
9	0.04895889279829302
10	0.057270943627853954
11	0.05526143683389417
12	0.06622238298276574
13	0.06339080522764058
14	0.06987603169905626
15	0.06092459234414448
16	0.06814054855881826
17	0.06375617009926964
18	0.07864478861815352
19	0.07508248111977027
20	0.08010624810466974
21	0.07654394060628647
22	0.0789188122718753
23	0.08321184951351668
24	0.07910149470768985
25	0.07727467034954458
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1094796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.10891830320234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68559571543582	36.67728818415337
2	14.805972041153003	14.542105427369616
3	4.901867025524469	7.2217616186892775
4	2.050642390260343	4.02819318449515
5	1.0197763629015046	2.504005704663341
6	0.6073664594344244	1.789626590188224
7	0.36492123524009706	1.2544620989956725
8	0.26012520579020687	1.0219573983803971
9	0.1835323751037196	0.8111768773465127
>10	0.9036471595096104	7.9147329336504075
>50	0.09092363477039907	3.1344996652565063
>100	0.10714984696130675	11.285551110708134
>500	0.013813742886013093	4.538440297689675
>1k	0.0046668050290584765	3.276198908413631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3738	0.3414334725373494	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2567	0.23447290636794435	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1934	0.17665391543264683	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1431	0.13070928282529348	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1422	0.12988721186412813	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1375	0.12559417462248676	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1342	0.12257991443154706	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1341	0.12248857321363979	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1340	0.12239723199573255	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1332	0.12166650225247443	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1320	0.12057040763758728	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1301	0.11883492449734928	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1299	0.11865224206153474	No Hit
GAACTACGACGGTATCTGATCGTCT	1297	0.11846955962572021	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1294	0.11819553597199844	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1260	0.11508993456315149	No Hit
TCGTAGTTCCGACCATAAACGATGC	1223	0.11171030950058276	No Hit
GTACATGGGGAATAATTGCAATCCC	1218	0.11125360341104644	No Hit
GTACATGGGGTGGTATCAACGCAAA	1198	0.10942677905290117	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1146	0.1046770357217235	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1128	0.10303289379939276	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1108	0.10120606944124749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.13412179072631E-5	0.0
6	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
7	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
8	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
9	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
10	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
11	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
12	0.0	0.0	0.0	9.13412179072631E-5	5.480473074435785E-4
13	0.0	0.0	0.0	1.826824358145262E-4	6.393885253508417E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	50	8.709114E-5	15.20022	1
TACCTAT	50	8.709114E-5	15.20022	6
CGGGTAA	60	2.5643296E-5	14.251509	19
CCGTCCG	40	0.005275679	14.250856	9
GTATAGA	60	2.5665408E-5	14.250206	1
ACGAACG	130	1.8189894E-12	13.886085	15
CGCAAGG	55	1.95756E-4	13.819645	16
GCGTTAT	160	0.0	13.656446	1
CCGTCGT	205	0.0	13.439833	9
CGAACGA	135	1.8189894E-12	13.371786	16
CGGACAT	165	0.0	13.239591	5
CGAGCCG	230	0.0	13.21879	15
CCGGTTT	65	5.4442036E-5	13.155238	12
TCCAACG	110	2.737579E-9	12.955916	18
GATAACG	140	3.6379788E-12	12.893632	11
CGATAAC	140	3.6379788E-12	12.893632	10
CGTTATT	170	0.0	12.849604	2
ACCGTCG	230	0.0	12.804533	8
TGCGTAC	60	4.0882317E-4	12.667429	11
TAACGAA	150	1.8189894E-12	12.667427	13
>>END_MODULE