FastQCFastQC Report
Thu 2 Feb 2017
SRR4064238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094796
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16070.1467853371769718No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14580.1331754957087896No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14000.12787770507016832No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13890.12687295167318843No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13300.1214838198166599No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12580.11490725212733696No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12420.11344579264082075No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12410.11335445142291349No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12390.11317176898709898No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12110.11061421488569559No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10970.1002013160442676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.10001863360845308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA407.0314476E-427.50017410
AGTCCGC508.3403684E-526.40016610
TATAGGG852.3541725E-723.2921352
TAATACC1205.984475E-1022.0001374
TTAGGAC2350.021.5310653
TAGGACG2150.021.4885044
GGCGTTA4300.020.97495842
AGGACGT2450.019.7552245
GTATAAT1504.656613E-1019.0676561
CGGCGTT4850.019.05166641
GTATAGG1051.7914826E-618.8581221
TAATACT2200.018.0001134
GTATCAA24900.017.8483181
CGCCCCT2850.017.7544965
GTATAAA1254.8488073E-717.6009121
TAGGACA2003.6379788E-1217.6001114
ATGCGGC5100.017.25658435
CTTATAT902.2157987E-417.1104353
CCTAATA902.216572E-417.1096532
CGCAATC902.216572E-417.10965344
Produced by FastQC (version 0.11.5)