##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1094796
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276547411572565	32.0	32.0	32.0	32.0	32.0
2	31.388863313347876	32.0	32.0	32.0	32.0	32.0
3	31.457908139963976	32.0	32.0	32.0	32.0	32.0
4	31.547196920704863	32.0	32.0	32.0	32.0	32.0
5	31.493220654806922	32.0	32.0	32.0	32.0	32.0
6	35.05727551068875	36.0	36.0	36.0	36.0	36.0
7	35.06982305379267	36.0	36.0	36.0	36.0	36.0
8	35.029965399946654	36.0	36.0	36.0	36.0	36.0
9	35.12137238353081	36.0	36.0	36.0	36.0	36.0
10	34.98513513019777	36.0	36.0	36.0	36.0	36.0
11	35.12895279120494	36.0	36.0	36.0	36.0	36.0
12	35.02168623195554	36.0	36.0	36.0	36.0	36.0
13	35.07789761745567	36.0	36.0	36.0	36.0	36.0
14	35.01034621975236	36.0	36.0	36.0	36.0	36.0
15	34.98851384184816	36.0	36.0	36.0	36.0	36.0
16	34.99427747269811	36.0	36.0	36.0	36.0	36.0
17	34.95419146580733	36.0	36.0	36.0	36.0	36.0
18	34.95450567959693	36.0	36.0	36.0	36.0	36.0
19	34.95047661847504	36.0	36.0	36.0	36.0	36.0
20	34.95013408890789	36.0	36.0	36.0	36.0	36.0
21	34.938041425069144	36.0	36.0	36.0	36.0	36.0
22	34.921876769736095	36.0	36.0	36.0	32.0	36.0
23	34.864205751573806	36.0	36.0	36.0	32.0	36.0
24	34.83945319493312	36.0	36.0	36.0	32.0	36.0
25	34.80714215251061	36.0	36.0	36.0	32.0	36.0
26	34.74482277976902	36.0	36.0	36.0	32.0	36.0
27	34.74851661862119	36.0	36.0	36.0	32.0	36.0
28	34.7169070767522	36.0	36.0	36.0	32.0	36.0
29	34.68917040252248	36.0	36.0	36.0	32.0	36.0
30	34.65799199120202	36.0	36.0	36.0	32.0	36.0
31	34.6546187600247	36.0	36.0	36.0	32.0	36.0
32	34.60599052243523	36.0	36.0	36.0	32.0	36.0
33	34.58205638310699	36.0	36.0	36.0	32.0	36.0
34	34.57623520728976	36.0	36.0	36.0	32.0	36.0
35	34.52253570528208	36.0	36.0	36.0	32.0	36.0
36	34.48762965885882	36.0	36.0	36.0	32.0	36.0
37	34.48019631054552	36.0	36.0	36.0	32.0	36.0
38	34.42650228901092	36.0	36.0	36.0	32.0	36.0
39	34.43075056905579	36.0	36.0	36.0	32.0	36.0
40	34.394724679300985	36.0	36.0	36.0	32.0	36.0
41	34.382494090223204	36.0	36.0	36.0	32.0	36.0
42	34.29598664956759	36.0	36.0	36.0	32.0	36.0
43	34.28114735530637	36.0	36.0	36.0	32.0	36.0
44	34.2081602417254	36.0	36.0	36.0	32.0	36.0
45	34.1845037796996	36.0	36.0	36.0	32.0	36.0
46	34.138033021677096	36.0	36.0	36.0	32.0	36.0
47	34.10702267819758	36.0	36.0	36.0	32.0	36.0
48	34.04507871786159	36.0	36.0	36.0	32.0	36.0
49	34.02042572314842	36.0	36.0	36.0	32.0	36.0
50	33.41667032031538	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	13.0
21	46.0
22	136.0
23	393.0
24	1103.0
25	2229.0
26	4709.0
27	8481.0
28	14007.0
29	21130.0
30	30180.0
31	42311.0
32	59502.0
33	95120.0
34	192177.0
35	623253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41454636139995	18.415574046787548	12.115268847064884	26.054610744747613
2	15.874781808067825	20.961496830907763	36.79115595368257	26.372565407341845
3	17.985018117646415	24.8551090666786	29.014906068657982	28.144966747017
4	12.130296420520352	16.253438996854207	36.14554675026215	35.47071783236329
5	14.178714573308634	37.00872125948578	33.20445087486619	15.608113292339395
6	33.674950424418135	36.301017635297264	16.660135751690046	13.363896188594557
7	29.582707264830404	30.827780543389398	21.192460688987435	18.39705150279276
8	27.9843002714661	32.61466063083899	19.698373030226634	19.70266606746828
9	27.63868806158697	13.795046845156595	18.59849083420642	39.96777425905002
10	16.002806035533983	26.622262128659287	31.323559098013032	26.0513727377937
11	37.62107278433608	21.000350750276763	22.413764756173755	18.964811709213407
12	24.876620737215642	23.87798628967067	28.721998182308106	22.523394790805582
13	29.446834552745276	19.252185350615186	25.43930799514062	25.861672101498918
14	23.72501128066102	19.980745236090076	25.270324828232525	31.023918655016374
15	25.171456862659124	27.32302582540448	22.451166308589467	25.054351003346927
16	25.63916601052626	25.62363786164602	23.994369675900128	24.74282645192759
17	23.734650108330683	25.72196098633901	25.336592387988265	25.206796517342045
18	24.866070572245164	24.648038487641042	26.550955294745215	23.934935645368576
19	25.671540634054196	24.451770010120608	25.581934899287173	24.294754456538023
20	25.9181923059006	23.948128722756117	25.086797705855112	25.046881265488175
21	27.35961768219833	23.924091794270346	24.54320256924578	24.173087954285545
22	26.146999746983674	23.98329909544379	25.172475842421065	24.697225315151478
23	24.427217349128185	23.815134816178855	25.82347068425182	25.934177150441133
24	24.89940984061769	24.82925933673064	25.504092569435755	24.76723825321592
25	24.891895496239407	24.192116363742656	25.736270682350877	25.17971745766706
26	24.61027062281796	25.337935973487625	26.027053147157208	24.024740256537207
27	25.27349359290561	24.511109649555504	25.154924894311257	25.06047186322763
28	24.315661301328255	24.672914099473576	26.09863684901388	24.91278775018429
29	24.25909027383859	24.542255032796874	25.93615639463668	25.26249829872786
30	24.191846715548497	25.121648889380122	26.180521745167155	24.505982649904222
31	25.184271705421047	24.69190611254984	24.88026563991541	25.243556542113698
32	24.8416085410813	24.786710463336124	24.7360142351355	25.63566676044708
33	24.389695827597578	24.372431470092014	25.583950907109536	25.653921795200873
34	25.196001684385905	24.509093765671228	25.77603696149205	24.518867588450814
35	25.809583835726553	24.35775877817969	25.798439840695675	24.03421754539808
36	24.45202232195464	25.210672810167466	25.19185498950861	25.14544987836928
37	25.580314080266582	24.93331823097834	25.033706264067114	24.452661424687967
38	24.503679073357663	24.795264477644665	25.479923815091738	25.221132633905942
39	25.3785128043517	24.536124850992696	24.83318032966581	25.252182014989792
40	25.680535421821894	24.889679318720127	25.430892348389563	23.998892911068424
41	24.445491863925596	25.229412269788465	25.92956956540008	24.395526300885862
42	25.942645225230986	25.567313533041336	25.070951300050698	23.419089941676983
43	24.802906146697566	24.46667513105337	25.496833619082505	25.23358510316656
44	24.390745172546083	25.223880597014926	24.94729534701036	25.438078883428634
45	24.587245462326187	25.35863402568007	25.144252858339446	24.9098676536543
46	24.24934690074718	25.065401267834638	25.129615082482324	25.55563674893586
47	24.736313754433592	24.796874586099143	25.62691422010346	24.83989743936381
48	25.62040315243722	25.38062719908803	24.36626073739555	24.632708911079202
49	24.593187192806663	25.81405948715326	24.656669629180364	24.936083690859718
50	24.743417831592946	25.903746519836023	24.50272932545105	24.85010632311998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	136.5
2	59.0
3	146.5
4	234.0
5	256.5
6	279.0
7	266.5
8	254.0
9	362.0
10	470.0
11	668.5
12	867.0
13	1541.0
14	2215.0
15	3032.0
16	3849.0
17	4102.5
18	4356.0
19	4345.0
20	4334.0
21	4732.0
22	5130.0
23	5716.5
24	6303.0
25	6971.0
26	7639.0
27	9413.0
28	11187.0
29	13007.5
30	14828.0
31	16445.5
32	18063.0
33	20839.5
34	23616.0
35	27280.5
36	30945.0
37	35871.5
38	40798.0
39	44134.0
40	47470.0
41	51216.5
42	54963.0
43	55578.5
44	56194.0
45	61876.0
46	67558.0
47	72752.0
48	77946.0
49	80376.0
50	82806.0
51	79330.0
52	75854.0
53	75568.0
54	75282.0
55	76948.5
56	78615.0
57	77080.5
58	75546.0
59	69055.0
60	62564.0
61	55193.5
62	47823.0
63	41667.0
64	35511.0
65	30376.0
66	25241.0
67	21513.5
68	17786.0
69	16372.5
70	14959.0
71	11477.0
72	7995.0
73	6898.0
74	5801.0
75	4411.5
76	3022.0
77	2700.5
78	2379.0
79	1993.5
80	1608.0
81	1299.0
82	990.0
83	833.0
84	676.0
85	468.5
86	261.0
87	186.0
88	111.0
89	89.5
90	68.0
91	58.0
92	48.0
93	36.5
94	25.0
95	27.5
96	30.0
97	20.0
98	10.0
99	12.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009408145444448098
2	2.740236537217893E-4
3	6.393885253508416E-4
4	0.0
5	0.0
6	2.740236537217893E-4
7	9.13412179072631E-5
8	0.0
9	0.0017354831402379986
10	0.0011874358327944202
11	0.0
12	9.13412179072631E-5
13	5.480473074435786E-4
14	1.826824358145262E-4
15	0.005480473074435785
16	9.134121790726309E-4
17	0.0
18	2.740236537217893E-4
19	0.0
20	8.220709611653677E-4
21	0.0
22	4.5670608953631546E-4
23	8.220709611653677E-4
24	6.393885253508416E-4
25	0.002009506793959788
26	0.005663155510250311
27	0.006576567689322942
28	0.004841084549084944
29	0.0024662128834961033
30	0.004293037241641365
31	0.007398638650488311
32	0.0036536487162905236
33	0.00502376698489947
34	0.0031969426267542084
35	0.003288283844661471
36	0.008129368393746415
37	0.004019013587919576
38	0.00831205082956094
39	0.004658402113270417
40	0.003014260190939682
41	0.0038363311521050496
42	0.0028315777551251556
43	0.0011874358327944202
44	0.0014614594865162095
45	0.0015528007044234725
46	0.0014614594865162095
47	0.0026488953193106297
48	0.0021921892297743144
49	4.5670608953631546E-4
50	0.0018268243581452618
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1094796.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.77158290067955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9476399947416	40.07877180093472
2	14.26570244937052	15.056473988867674
3	4.638270270640026	7.343065923085125
4	1.9365349584713063	4.087760604041303
5	0.9805130058435384	2.5871611686533384
6	0.5219731314226493	1.6527209026078584
7	0.34402195278219516	1.2708208100629492
8	0.23612903812154587	0.9968722488391103
9	0.16994770986885854	0.8071568694112593
>10	0.7728750688203566	7.275411422531228
>50	0.08397456165215988	3.1360851374181875
>100	0.0886576367856009	9.94308978840909
>500	0.010624981142465026	3.751063419559148
>1k	0.0031352403371208274	2.0135459155789825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1607	0.1467853371769718	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1458	0.1331754957087896	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1400	0.12787770507016832	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1389	0.12687295167318843	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1330	0.1214838198166599	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1258	0.11490725212733696	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1242	0.11344579264082075	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1241	0.11335445142291349	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1239	0.11317176898709898	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1211	0.11061421488569559	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1097	0.1002013160442676	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1095	0.10001863360845308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.826824358145262E-4
2	0.0	0.0	0.0	0.0	1.826824358145262E-4
3	0.0	0.0	0.0	0.0	1.826824358145262E-4
4	0.0	0.0	0.0	0.0	1.826824358145262E-4
5	0.0	0.0	0.0	9.13412179072631E-5	2.7402365372178924E-4
6	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
7	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
8	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
9	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
10	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
11	0.0	0.0	0.0	9.13412179072631E-5	3.653648716290524E-4
12	0.0	0.0	0.0	9.13412179072631E-5	5.480473074435785E-4
13	0.0	0.0	0.0	1.826824358145262E-4	5.480473074435785E-4
14	0.0	0.0	0.0	1.826824358145262E-4	5.480473074435785E-4
15	0.0	0.0	0.0	1.826824358145262E-4	6.393885253508417E-4
16	0.0	0.0	0.0	1.826824358145262E-4	6.393885253508417E-4
17	0.0	0.0	0.0	1.826824358145262E-4	6.393885253508417E-4
18	0.0	0.0	0.0	2.7402365372178924E-4	6.393885253508417E-4
19	0.0	0.0	0.0	2.7402365372178924E-4	6.393885253508417E-4
20	0.0	0.0	0.0	2.7402365372178924E-4	6.393885253508417E-4
21	0.0	0.0	0.0	2.7402365372178924E-4	6.393885253508417E-4
22	0.0	0.0	0.0	5.480473074435785E-4	6.393885253508417E-4
23	0.0	0.0	0.0	0.0011874358327944202	6.393885253508417E-4
24	0.0	0.0	0.0	0.002922918973032419	7.307297432581048E-4
25	0.0	0.0	0.0	0.003196942626754208	7.307297432581048E-4
26	0.0	0.0	0.0	0.004201696023734102	8.220709611653678E-4
27	0.0	0.0	0.0	0.005206449420713996	9.134121790726309E-4
28	0.0	0.0	0.0	0.009225463008633573	9.134121790726309E-4
29	0.0	0.0	0.0	0.01945567941424704	9.134121790726309E-4
30	0.0	0.0	0.0	0.0383633115210505	9.134121790726309E-4
31	0.0	0.0	0.0	0.073255656761625	9.134121790726309E-4
32	0.0	0.0	0.0	0.11244103924384086	0.001096094614887157
33	0.0	0.0	0.0	0.14815545544558073	0.001096094614887157
34	0.0	0.0	0.0	0.18743217914570387	0.0011874358327944202
35	0.0	0.0	0.0	0.23173266983072646	0.0011874358327944202
36	0.0	0.0	0.0	0.28936897833020947	0.0011874358327944202
37	0.0	0.0	0.0	0.3738596048944278	0.0011874358327944202
38	0.0	0.0	0.0	0.4964395193259749	0.0012787770507016833
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCTA	40	7.0314476E-4	27.500174	10
AGTCCGC	50	8.3403684E-5	26.400166	10
TATAGGG	85	2.3541725E-7	23.292135	2
TAATACC	120	5.984475E-10	22.000137	4
TTAGGAC	235	0.0	21.531065	3
TAGGACG	215	0.0	21.488504	4
GGCGTTA	430	0.0	20.974958	42
AGGACGT	245	0.0	19.755224	5
GTATAAT	150	4.656613E-10	19.067656	1
CGGCGTT	485	0.0	19.051666	41
GTATAGG	105	1.7914826E-6	18.858122	1
TAATACT	220	0.0	18.000113	4
GTATCAA	2490	0.0	17.848318	1
CGCCCCT	285	0.0	17.754496	5
GTATAAA	125	4.8488073E-7	17.600912	1
TAGGACA	200	3.6379788E-12	17.600111	4
ATGCGGC	510	0.0	17.256584	35
CTTATAT	90	2.2157987E-4	17.110435	3
CCTAATA	90	2.216572E-4	17.109653	2
CGCAATC	90	2.216572E-4	17.109653	44
>>END_MODULE