FastQCFastQC Report
Thu 2 Feb 2017
SRR4064237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635297
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32200.5068495522566611No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27720.4363313536818213No Hit
TATCAACGCAGAGTACTTTTTTTTT23190.36502612164074444No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18280.287739435256266No Hit
GTACATGGGGTGGTATCAACGCAAA14980.23579522648462056No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14810.2331193126994146No Hit
GGTATCAACGCAGAGTACTTTTTTT10990.1729899558789039No Hit
GTACATGGGTGGTATCAACGCAAAA8840.1391475168307107No Hit
GTATCAACGCAGAGTACATGGGGTG8680.1366290097387521No Hit
GCGCAAGACGGACCAGAGCGAAAGC8480.1334808758738039No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8220.12938830184937125No Hit
GAACTACGACGGTATCTGATCGTCT8180.1287586750763816No Hit
GTATCTGATCGTCTTCGAACCTCCG8070.12702720145066007No Hit
GAATAGGACCGCGGTTCTATTTTGT7940.12498091443844375No Hit
CTATTGGAGCTGGAATTACCGCGGC7490.11789761324231028No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7060.11112912543267166No Hit
GATTAAGAGGGACGGCCGGGGGCAT6990.11002727857993978No Hit
GTCCTATTCCATTATTCCTAGCTGC6930.10908283842045532No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6690.10530507778251748No Hit
GTACATGGAAGCAGTGGTATCAACG6610.10404582423653819No Hit
GAATAACGCCGCCGCATCGCCAGTC6480.10199953722432185No Hit
GTACATGGGGAATAATTGCAATCCC6460.10168472383782702No Hit
ATCAGATACCGTCGTAGTTCCGACC6430.1012125037580848No Hit
CTTTAATATACGCTATTGGAGCTGG6370.10026806359860034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC551.1292252E-515.5410713
TCTAGAT807.737981E-915.4295012
TTCTCGT653.3633733E-614.615869511
GTCCTAA400.005289954614.2437381
TAGGGTC759.643154E-713.9337964
ATCGCCA1750.013.57294916
TCGCCAG1700.013.412209517
TATAGGG852.7075475E-713.4048152
CCTTATA500.001503705513.2931082
CGTCTTA655.4368596E-513.15531815
GCATCGC1750.013.03003114
CCTATTC1650.012.6630953
CTATTCC2150.012.3725044
AGCGTAT1001.4365651E-712.35040958
GTCCTAT2000.012.3445721
GCTCTTA853.9523165E-612.2887141
TATTCCA2250.012.2448515
TTAGAGT701.0886021E-412.2146914
TTGGACT550.00306424512.091314
TAGACTC951.0361291E-612.0003995
Produced by FastQC (version 0.11.5)