##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064237_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635297
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1387351112944	32.0	32.0	32.0	32.0	32.0
2	30.922124612582778	32.0	32.0	32.0	32.0	32.0
3	30.952954287522214	32.0	32.0	32.0	32.0	32.0
4	30.929135506700018	32.0	32.0	32.0	32.0	32.0
5	30.950106800441368	32.0	32.0	32.0	32.0	32.0
6	34.466838344900104	36.0	36.0	36.0	32.0	36.0
7	34.38476019877317	36.0	36.0	36.0	32.0	36.0
8	34.3451456562836	36.0	36.0	36.0	32.0	36.0
9	34.419163005649324	36.0	36.0	36.0	32.0	36.0
10	34.239829560032554	36.0	36.0	36.0	32.0	36.0
11	34.44462511234903	36.0	36.0	36.0	32.0	36.0
12	34.31034933267432	36.0	36.0	36.0	32.0	36.0
13	34.38344900101842	36.0	36.0	36.0	32.0	36.0
14	34.30152511345087	36.0	36.0	36.0	32.0	36.0
15	34.26664851242805	36.0	36.0	36.0	32.0	36.0
16	34.24951321980113	36.0	36.0	36.0	32.0	36.0
17	34.18943265905553	36.0	36.0	36.0	32.0	36.0
18	34.17765076806596	36.0	36.0	36.0	32.0	36.0
19	34.17525189006087	36.0	36.0	36.0	32.0	36.0
20	34.10790386228803	36.0	36.0	36.0	32.0	36.0
21	34.07538993573085	36.0	36.0	36.0	32.0	36.0
22	34.07160587882518	36.0	36.0	36.0	32.0	36.0
23	34.054772807049304	36.0	36.0	36.0	32.0	36.0
24	34.02783579963387	36.0	36.0	36.0	32.0	36.0
25	33.4940697028319	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	14.0
6	20.0
7	6.0
8	34.0
9	49.0
10	68.0
11	18.0
12	44.0
13	36.0
14	106.0
15	211.0
16	353.0
17	424.0
18	552.0
19	742.0
20	863.0
21	1215.0
22	1590.0
23	2262.0
24	3046.0
25	4265.0
26	5958.0
27	7973.0
28	11298.0
29	15374.0
30	21145.0
31	29870.0
32	44660.0
33	67502.0
34	151363.0
35	264230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.43697889192445	17.11887759520679	11.496460992181904	24.947682520686858
2	16.851263581096184	19.97849692565855	37.25226600845641	25.91797348478886
3	18.881230058225654	24.803277888576343	28.2321218598838	28.083370193314206
4	12.507634573125042	15.122436514982654	35.21301246088265	37.15691645100965
5	14.888522623697147	36.74133124278817	32.64398865942218	15.726157474092508
6	34.00382553663778	34.94100329814784	17.174455490747082	13.880715674467298
7	30.102652916633865	30.64787845390853	20.94953947886326	18.299929150594348
8	28.928741829473577	31.47953648255196	19.679178404458185	19.912543283516282
9	27.890294029639197	13.745688772697923	18.76198875537427	39.60202844228861
10	16.5450255777033	25.96640878965804	30.731358516241215	26.757207116397453
11	37.15135755764049	21.355525910852744	22.304138901007516	19.188977630499256
12	25.135967364682347	23.711479850998984	28.369415415147387	22.78313736917128
13	29.08192275040439	19.30420867439593	25.943648084825554	25.670220490374117
14	23.97418753258454	19.45653320984679	25.69370612408192	30.875573133486746
15	25.191404779567527	26.486861938129913	23.201047075889377	25.120686206413183
16	25.935119673897294	25.528436332478595	23.659142027305432	24.877301966318683
17	23.468548783407467	25.50040322072554	25.615699146936155	25.415348848930837
18	24.712709767159044	24.37985479142611	26.82184146147436	24.085593979940487
19	25.340997339441014	24.729651606968176	25.98384761880758	23.945503434783223
20	25.948108823390413	23.58338978244774	25.41297121882827	25.055530175333573
21	27.378413204497697	23.584546024504817	24.701093548661497	24.335947222335992
22	26.021616154933103	23.59503657040058	25.331165731730948	25.05218154293537
23	23.90135164939034	23.966413560603677	26.36803301931378	25.764201770692207
24	24.620832624462047	24.943762877828394	25.813941765436066	24.621462732273493
25	24.36858562865762	24.37567441970369	26.040595143390487	25.2151448082482
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	149.0
1	149.0
2	415.5
3	682.0
4	682.0
5	682.0
6	1980.5
7	3279.0
8	3279.0
9	3279.0
10	3027.5
11	2776.0
12	2776.0
13	2776.0
14	2247.0
15	1718.0
16	1718.0
17	1718.0
18	2962.5
19	4207.0
20	4207.0
21	4207.0
22	6594.0
23	8981.0
24	8981.0
25	8981.0
26	13218.0
27	17455.0
28	17455.0
29	17455.0
30	21330.0
31	25205.0
32	25205.0
33	25205.0
34	30940.5
35	36676.0
36	36676.0
37	36676.0
38	41880.0
39	47084.0
40	47084.0
41	47084.0
42	55957.5
43	64831.0
44	64831.0
45	64831.0
46	75348.0
47	85865.0
48	85865.0
49	85865.0
50	87035.0
51	88205.0
52	88205.0
53	88205.0
54	80616.0
55	73027.0
56	73027.0
57	73027.0
58	67236.5
59	61446.0
60	61446.0
61	61446.0
62	54743.0
63	48040.0
64	48040.0
65	48040.0
66	39928.0
67	31816.0
68	31816.0
69	31816.0
70	23831.5
71	15847.0
72	15847.0
73	15847.0
74	12087.5
75	8328.0
76	8328.0
77	8328.0
78	7073.5
79	5819.0
80	5819.0
81	5819.0
82	3859.5
83	1900.0
84	1900.0
85	1900.0
86	1496.0
87	1092.0
88	1092.0
89	1092.0
90	759.0
91	426.0
92	426.0
93	426.0
94	277.0
95	128.0
96	128.0
97	128.0
98	221.5
99	315.0
100	315.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03651835283339919
2	0.0061388610366490005
3	0.0015740669324741025
4	0.004564794104174898
5	0.008814774821854976
6	0.014481415778761744
7	0.023138783907369308
8	0.037777606379378464
9	0.051471988691903156
10	0.058083069808294384
11	0.0582404765015418
12	0.06327749068545893
13	0.06201823713947965
14	0.06453674423143821
15	0.060758983593500356
16	0.06374971076520115
17	0.0643793375381908
18	0.07649965291824139
19	0.073666332439788
20	0.079805193476437
21	0.07602743283849916
22	0.07775890646422066
23	0.08137926040891111
24	0.07634224622499397
25	0.07744409307772585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	635297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.21826005352163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.14169412822649	30.03478543353559
2	15.16340696749211	12.803453173019275
3	5.4997866871093475	6.965742737858359
4	2.617872912871852	4.420881576907761
5	1.388340954140712	2.9306669722433463
6	0.8587891759470165	2.1753950856768416
7	0.5884405765748085	1.739005610151563
8	0.3840352710674856	1.2970640754921423
9	0.29938730929601903	1.1375650152525103
>10	1.7234939044803714	13.646862963140183
>50	0.16849938626584346	4.98215496046693
>100	0.1523983326506579	12.456177746334287
>500	0.011233292332972322	3.1627736299373064
>1k	0.0026211015443602086	2.24747101998394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3220	0.5068495522566611	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2772	0.4363313536818213	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2319	0.36502612164074444	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1828	0.287739435256266	No Hit
GTACATGGGGTGGTATCAACGCAAA	1498	0.23579522648462056	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1481	0.2331193126994146	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1099	0.1729899558789039	No Hit
GTACATGGGTGGTATCAACGCAAAA	884	0.1391475168307107	No Hit
GTATCAACGCAGAGTACATGGGGTG	868	0.1366290097387521	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	848	0.1334808758738039	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	822	0.12938830184937125	No Hit
GAACTACGACGGTATCTGATCGTCT	818	0.1287586750763816	No Hit
GTATCTGATCGTCTTCGAACCTCCG	807	0.12702720145066007	No Hit
GAATAGGACCGCGGTTCTATTTTGT	794	0.12498091443844375	No Hit
CTATTGGAGCTGGAATTACCGCGGC	749	0.11789761324231028	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	706	0.11112912543267166	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	699	0.11002727857993978	No Hit
GTCCTATTCCATTATTCCTAGCTGC	693	0.10908283842045532	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	669	0.10530507778251748	No Hit
GTACATGGAAGCAGTGGTATCAACG	661	0.10404582423653819	No Hit
GAATAACGCCGCCGCATCGCCAGTC	648	0.10199953722432185	No Hit
GTACATGGGGAATAATTGCAATCCC	646	0.10168472383782702	No Hit
ATCAGATACCGTCGTAGTTCCGACC	643	0.1012125037580848	No Hit
CTTTAATATACGCTATTGGAGCTGG	637	0.10026806359860034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.148133864948205E-4
2	0.0	0.0	0.0	0.0	3.148133864948205E-4
3	0.0	0.0	0.0	0.0	3.148133864948205E-4
4	0.0	0.0	0.0	0.0	3.148133864948205E-4
5	0.0	0.0	0.0	0.0	3.148133864948205E-4
6	0.0	0.0	0.0	0.0	6.29626772989641E-4
7	0.0	0.0	0.0	0.0	6.29626772989641E-4
8	0.0	0.0	0.0	0.0	7.870334662370514E-4
9	0.0	0.0	0.0	0.0	9.444401594844616E-4
10	0.0	0.0	0.0	0.0	9.444401594844616E-4
11	0.0	0.0	0.0	0.0	9.444401594844616E-4
12	0.0	0.0	0.0	0.0	9.444401594844616E-4
13	0.0	0.0	0.0	0.0	9.444401594844616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATC	55	1.1292252E-5	15.541071	3
TCTAGAT	80	7.737981E-9	15.429501	2
TTCTCGT	65	3.3633733E-6	14.6158695	11
GTCCTAA	40	0.0052899546	14.243738	1
TAGGGTC	75	9.643154E-7	13.933796	4
ATCGCCA	175	0.0	13.572949	16
TCGCCAG	170	0.0	13.4122095	17
TATAGGG	85	2.7075475E-7	13.404815	2
CCTTATA	50	0.0015037055	13.293108	2
CGTCTTA	65	5.4368596E-5	13.155318	15
GCATCGC	175	0.0	13.030031	14
CCTATTC	165	0.0	12.663095	3
CTATTCC	215	0.0	12.372504	4
AGCGTAT	100	1.4365651E-7	12.3504095	8
GTCCTAT	200	0.0	12.344572	1
GCTCTTA	85	3.9523165E-6	12.288714	1
TATTCCA	225	0.0	12.244851	5
TTAGAGT	70	1.0886021E-4	12.214691	4
TTGGACT	55	0.003064245	12.09131	4
TAGACTC	95	1.0361291E-6	12.000399	5
>>END_MODULE