FastQCFastQC Report
Thu 2 Feb 2017
SRR4064237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635297
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10740.16905478854771863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.14780488495931823No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8470.1333234691805565No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7880.1240364742789593No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7880.1240364742789593No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7640.12025871364102145No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7640.12025871364102145No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7330.11537910615035173No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC7120.1120735655921561No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6890.10845321164746567No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6830.10750877148798121No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6470.10184213053107444No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6410.10089769037158998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA300.00574414229.33212717
GAATTCG300.00574414229.33212743
AATTCGT500.002578539822.00082622
TTCGCGC500.002579718821.99909617
TAACCTA703.2132775E-521.9990945
GTAGAGT703.2132775E-521.9990944
GTGTAGG803.6148303E-621.9973641
GTTCGCG500.002580898321.99736216
GTCTAGA1601.8189894E-1220.6225281
TAGTTCG755.4658136E-520.5341059
AACGGAC654.929988E-420.30685815
CGATTAA550.004476255320.003933
ACGATTA550.004476255320.003932
GTCGTAA550.004482378319.99917838
TATACAG550.004482378319.9991785
TAGTACT550.004482378319.9991784
TATAACC550.004484420619.9976023
TTACTTG1352.586603E-919.55782928
GTACACT901.0010888E-519.554756
CTAGAAG2000.018.6977583
Produced by FastQC (version 0.11.5)