##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635297
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.105923686086978	32.0	32.0	32.0	32.0	32.0
2	31.193972268088785	32.0	32.0	32.0	32.0	32.0
3	31.332584602162452	32.0	32.0	32.0	32.0	32.0
4	31.465065945534135	32.0	32.0	32.0	32.0	32.0
5	31.3415347467405	32.0	32.0	32.0	32.0	32.0
6	34.923841919606105	36.0	36.0	36.0	36.0	36.0
7	34.95775046946546	36.0	36.0	36.0	36.0	36.0
8	34.88877800461831	36.0	36.0	36.0	32.0	36.0
9	35.045797477400335	36.0	36.0	36.0	36.0	36.0
10	34.83774203246671	36.0	36.0	36.0	32.0	36.0
11	35.035481042724896	36.0	36.0	36.0	36.0	36.0
12	34.9129257654294	36.0	36.0	36.0	32.0	36.0
13	34.96922699147013	36.0	36.0	36.0	36.0	36.0
14	34.894172331995904	36.0	36.0	36.0	32.0	36.0
15	34.865200056036784	36.0	36.0	36.0	32.0	36.0
16	34.871691508066306	36.0	36.0	36.0	32.0	36.0
17	34.8298071610601	36.0	36.0	36.0	32.0	36.0
18	34.83392649422239	36.0	36.0	36.0	32.0	36.0
19	34.8241987605797	36.0	36.0	36.0	32.0	36.0
20	34.814585933823075	36.0	36.0	36.0	32.0	36.0
21	34.80380357533563	36.0	36.0	36.0	32.0	36.0
22	34.8026922840813	36.0	36.0	36.0	32.0	36.0
23	34.73918025742291	36.0	36.0	36.0	32.0	36.0
24	34.71255019305931	36.0	36.0	36.0	32.0	36.0
25	34.68150644501706	36.0	36.0	36.0	32.0	36.0
26	34.6046573492398	36.0	36.0	36.0	32.0	36.0
27	34.6097951037074	36.0	36.0	36.0	32.0	36.0
28	34.57928968655605	36.0	36.0	36.0	32.0	36.0
29	34.546704926986905	36.0	36.0	36.0	32.0	36.0
30	34.519349217767434	36.0	36.0	36.0	32.0	36.0
31	34.51120499545882	36.0	36.0	36.0	32.0	36.0
32	34.444703815695654	36.0	36.0	36.0	32.0	36.0
33	34.389597306456665	36.0	36.0	36.0	32.0	36.0
34	34.359463369101384	36.0	36.0	36.0	32.0	36.0
35	34.296220507888435	36.0	36.0	36.0	32.0	36.0
36	34.26997451585636	36.0	36.0	36.0	32.0	36.0
37	34.23824447463155	36.0	36.0	36.0	32.0	36.0
38	34.17532429713976	36.0	36.0	36.0	32.0	36.0
39	34.176890493737574	36.0	36.0	36.0	32.0	36.0
40	34.12681155428091	36.0	36.0	36.0	32.0	36.0
41	34.09550021486014	36.0	36.0	36.0	32.0	36.0
42	33.988543940865455	36.0	36.0	36.0	32.0	36.0
43	33.971422814840935	36.0	36.0	36.0	32.0	36.0
44	33.88120831674004	36.0	36.0	36.0	32.0	36.0
45	33.82094673829721	36.0	36.0	36.0	32.0	36.0
46	33.78190043397025	36.0	36.0	36.0	32.0	36.0
47	33.71127205071014	36.0	36.0	36.0	27.0	36.0
48	33.64529188710162	36.0	36.0	36.0	27.0	36.0
49	33.601726436611536	36.0	36.0	36.0	27.0	36.0
50	32.90223470282403	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	4.0
21	25.0
22	49.0
23	214.0
24	563.0
25	1380.0
26	2899.0
27	5374.0
28	9302.0
29	14254.0
30	20041.0
31	28561.0
32	41725.0
33	67656.0
34	130838.0
35	312404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.926888741615514	18.320947153172526	12.282231747765843	25.469932357446112
2	15.84056490469752	20.94262017683179	36.472147497296525	26.744667421174167
3	18.006022418002402	25.418507680707958	28.68282823830894	27.892641662980704
4	12.160611493521928	15.839992948180143	35.44216327166664	36.55723228663129
5	14.455443674375923	37.03810973450213	32.35258469660647	16.15386189451548
6	34.1621320421787	35.448931759476274	16.618369046288585	13.77056715205644
7	29.913095764658102	30.721851354563302	21.085256187263596	18.279796693515003
8	28.813137792245204	31.680458116440025	19.503633733513617	20.002770357801154
9	27.869345325700113	13.412551373932974	18.695625483044832	40.02247781732209
10	16.296102743143713	26.302981458948864	30.77776710903473	26.623148688872693
11	37.581320232898946	21.174033562255133	22.195288188044334	19.04935801680159
12	25.034117900761377	23.692540654213698	28.35256580780328	22.920775637221645
13	29.25585751501271	19.278130632225974	25.973602814440532	25.49240903832078
14	24.10691096262366	19.320315758820705	25.563871902029767	31.008901376525866
15	25.272995449157598	26.609126556235406	23.06872543355786	25.04915256104913
16	25.941450914219516	25.472220823049412	23.73917040245807	24.847157860273008
17	23.668457430146844	25.382458912917894	25.571504351508032	25.37757930542723
18	24.867817313216694	24.15649422709135	27.000055092516074	23.975633367175877
19	25.475841182692793	24.576890142547725	26.14340401954365	23.803864655215836
20	25.947869723104144	23.211966761793377	25.750480486956413	25.08968302814607
21	27.295265049260426	23.334597833769088	25.168858030181156	24.201279086789327
22	26.0464823428486	23.437615595904266	25.625575520034	24.89032654121314
23	23.910138252474866	23.4531794291399	26.764910977243357	25.87177134114188
24	24.751413914524477	24.625330359377486	26.063249555716812	24.560006170381225
25	24.617496316631197	23.778664164914183	26.42376371031721	25.180075808137413
26	24.536297336395563	24.778085434943105	26.567884055050868	24.117733173610468
27	25.144192928879516	24.343733274564745	25.5408808991594	24.971192897396342
28	24.05327506930135	24.383843694560888	26.861219420434495	24.701661815703268
29	24.130261287595804	24.472631623685416	26.799805439202114	24.597301649516666
30	24.30888524394045	24.586716366693008	26.5699426078032	24.534455781563345
31	24.735891797100674	24.589020717795012	25.270641053693737	25.404446431410577
32	24.520250157804682	24.436192601990623	25.44944819520242	25.594109045002273
33	24.084513028462226	24.086874255229723	26.13421527776685	25.6943974385412
34	25.019794514833688	24.265792717192657	26.288060169028626	24.426352598945027
35	25.931487830447963	24.129115940523302	25.68057247738771	24.258823751641025
36	24.574494210098795	25.320821605556294	25.336721007990633	24.767963176354282
37	25.610439491247956	25.02062082861101	25.102159677622467	24.266780002518573
38	24.49850531522186	24.734789870460652	25.784611122217832	24.982093692099657
39	24.90059470027295	24.835268968860877	25.280585822269934	24.983550508596235
40	25.228678332895203	25.132185599758216	25.573879905112857	24.06525616223372
41	24.107002310806642	25.697649525560546	25.874737909192223	24.320610254440588
42	25.96039875142259	25.569706729127017	24.99752078994716	23.47237372950323
43	24.8250394310594	24.71579729444691	25.085237200253115	25.373926074240575
44	24.54213895679303	25.46880091170818	24.926057507504435	25.063002623994358
45	24.784900029278152	25.58296578234055	24.602619922365673	25.029514266015624
46	24.440530215382292	25.58347460761952	24.789820066143125	25.186175110855057
47	24.88831520455869	25.091693295764006	25.30073826876761	24.71925323090969
48	25.79150263112743	25.99739641952591	23.847002655526154	24.36409829382051
49	24.534782321255985	26.36244006711853	24.357226732819765	24.74555087880572
50	24.91220558767557	25.987303456211407	23.970275039312693	25.13021591680033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	40.0
2	25.0
3	91.0
4	157.0
5	194.0
6	231.0
7	239.0
8	247.0
9	344.5
10	442.0
11	687.0
12	932.0
13	1564.5
14	2197.0
15	2994.0
16	3791.0
17	4204.0
18	4617.0
19	4604.5
20	4592.0
21	4653.0
22	4714.0
23	4966.0
24	5218.0
25	4862.5
26	4507.0
27	4887.0
28	5267.0
29	5935.0
30	6603.0
31	7557.0
32	8511.0
33	9850.0
34	11189.0
35	12612.0
36	14035.0
37	17094.0
38	20153.0
39	22345.0
40	24537.0
41	27136.0
42	29735.0
43	30555.5
44	31376.0
45	36412.0
46	41448.0
47	44176.0
48	46904.0
49	47979.5
50	49055.0
51	46527.5
52	44000.0
53	44124.5
54	44249.0
55	45503.5
56	46758.0
57	46326.0
58	45894.0
59	41364.5
60	36835.0
61	32571.5
62	28308.0
63	24670.0
64	21032.0
65	17692.5
66	14353.0
67	12484.0
68	10615.0
69	9814.0
70	9013.0
71	6870.5
72	4728.0
73	4140.5
74	3553.0
75	2749.5
76	1946.0
77	1659.5
78	1373.0
79	1110.5
80	848.0
81	683.5
82	519.0
83	442.5
84	366.0
85	263.0
86	160.0
87	114.5
88	69.0
89	57.5
90	46.0
91	36.0
92	26.0
93	24.0
94	22.0
95	21.0
96	20.0
97	16.0
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007240707889380872
2	6.296267729896411E-4
3	6.296267729896411E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.296267729896411E-4
10	6.296267729896411E-4
11	0.0
12	0.0
13	3.148133864948206E-4
14	3.148133864948206E-4
15	0.004722200797422308
16	1.574066932474103E-4
17	0.0
18	3.148133864948206E-4
19	1.574066932474103E-4
20	6.296267729896411E-4
21	0.0
22	3.148133864948206E-4
23	0.0015740669324741025
24	6.296267729896411E-4
25	0.0014166602392266924
26	0.004722200797422308
27	0.00582404765015418
28	0.004407387410927488
29	0.003148133864948205
30	0.002990727171700795
31	0.007240707889380872
32	0.002833320478453385
33	0.005351827570411949
34	0.003148133864948205
35	0.003620353944690436
36	0.008342554742112743
37	0.0026759137852059747
38	0.007240707889380872
39	0.002990727171700795
40	0.0025185070919585646
41	0.0033055405581956156
42	0.002203693705463744
43	0.0017314736257215129
44	9.444401594844616E-4
45	0.0017314736257215129
46	0.0012592535459792823
47	0.004249980717680077
48	0.002833320478453385
49	4.722200797422308E-4
50	9.444401594844616E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	635297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.442205630976446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68573640169228	34.95813858439012
2	14.200271590410441	13.4738440961593
3	5.011264123616757	7.132362690712836
4	2.2856822282731124	4.337512251232058
5	1.2401309704840033	2.9417274255522234
6	0.7480007630296003	2.129208360706655
7	0.5130421953706016	1.703789733109777
8	0.3395803052823089	1.2888350937146447
9	0.25328168480732766	1.0814617595870462
>10	1.4567849966950703	12.929429435757417
>50	0.13927652681849234	4.648230914975218
>100	0.11861827824974089	10.71709218532632
>500	0.007996737859532971	2.4885936291220987
>1k	3.3319741081387383E-4	0.16977383965426754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1074	0.16905478854771863	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	939	0.14780488495931823	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	847	0.1333234691805565	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	788	0.1240364742789593	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	788	0.1240364742789593	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	764	0.12025871364102145	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	764	0.12025871364102145	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	733	0.11537910615035173	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	712	0.1120735655921561	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	689	0.10845321164746567	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	683	0.10750877148798121	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	647	0.10184213053107444	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	641	0.10089769037158998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.148133864948205E-4
2	0.0	0.0	0.0	0.0	3.148133864948205E-4
3	0.0	0.0	0.0	0.0	3.148133864948205E-4
4	0.0	0.0	0.0	0.0	4.722200797422308E-4
5	0.0	0.0	0.0	0.0	4.722200797422308E-4
6	0.0	0.0	0.0	0.0	6.29626772989641E-4
7	0.0	0.0	0.0	0.0	6.29626772989641E-4
8	0.0	0.0	0.0	0.0	7.870334662370514E-4
9	0.0	0.0	0.0	0.0	7.870334662370514E-4
10	0.0	0.0	0.0	0.0	7.870334662370514E-4
11	0.0	0.0	0.0	0.0	7.870334662370514E-4
12	0.0	0.0	0.0	0.0	7.870334662370514E-4
13	0.0	0.0	0.0	0.0	7.870334662370514E-4
14	0.0	0.0	0.0	0.0	0.001101846852731872
15	0.0	0.0	0.0	0.0	0.001259253545979282
16	0.0	0.0	0.0	0.0	0.0014166602392266924
17	0.0	0.0	0.0	0.0	0.0014166602392266924
18	0.0	0.0	0.0	0.0	0.0014166602392266924
19	0.0	0.0	0.0	0.0	0.0014166602392266924
20	0.0	0.0	0.0	0.0	0.0014166602392266924
21	0.0	0.0	0.0	1.5740669324741026E-4	0.0014166602392266924
22	0.0	0.0	0.0	6.29626772989641E-4	0.0014166602392266924
23	0.0	0.0	0.0	0.0017314736257215129	0.0020462870122163333
24	0.0	0.0	0.0	0.004092574024432667	0.0020462870122163333
25	0.0	0.0	0.0	0.0045647941041748975	0.0020462870122163333
26	0.0	0.0	0.0	0.005194420877164539	0.0020462870122163333
27	0.0	0.0	0.0	0.006768487809638642	0.0020462870122163333
28	0.0	0.0	0.0	0.008342554742112745	0.0020462870122163333
29	0.0	0.0	0.0	0.014953635858503976	0.0020462870122163333
30	0.0	0.0	0.0	0.02833320478453385	0.0020462870122163333
31	0.0	0.0	0.0	0.04674978789448085	0.0020462870122163333
32	0.0	0.0	0.0	0.06705525132339678	0.002203693705463744
33	0.0	0.0	0.0	0.0843699875806119	0.002203693705463744
34	0.0	0.0	0.0	0.10640692463524934	0.002203693705463744
35	0.0	0.0	0.0	0.1304901487021031	0.002203693705463744
36	0.0	0.0	0.0	0.16212889404483258	0.002203693705463744
37	0.0	0.0	0.0	0.208721275246066	0.002361100398711154
38	0.0	0.0	0.0	0.27404505294374126	0.002361100398711154
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGTA	30	0.005744142	29.332127	17
GAATTCG	30	0.005744142	29.332127	43
AATTCGT	50	0.0025785398	22.000826	22
TTCGCGC	50	0.0025797188	21.999096	17
TAACCTA	70	3.2132775E-5	21.999094	5
GTAGAGT	70	3.2132775E-5	21.999094	4
GTGTAGG	80	3.6148303E-6	21.997364	1
GTTCGCG	50	0.0025808983	21.997362	16
GTCTAGA	160	1.8189894E-12	20.622528	1
TAGTTCG	75	5.4658136E-5	20.534105	9
AACGGAC	65	4.929988E-4	20.306858	15
CGATTAA	55	0.0044762553	20.0039	33
ACGATTA	55	0.0044762553	20.0039	32
GTCGTAA	55	0.0044823783	19.999178	38
TATACAG	55	0.0044823783	19.999178	5
TAGTACT	55	0.0044823783	19.999178	4
TATAACC	55	0.0044844206	19.997602	3
TTACTTG	135	2.586603E-9	19.557829	28
GTACACT	90	1.0010888E-5	19.55475	6
CTAGAAG	200	0.0	18.697758	3
>>END_MODULE