FastQCFastQC Report
Thu 2 Feb 2017
SRR4064236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334899
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27000.2022624932672809No Hit
TATCAACGCAGAGTACTTTTTTTTT18860.14128409714892287No Hit
GCGCAAGACGGACCAGAGCGAAAGC14860.11131928333154793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA508.691509E-515.2042917
CTCGACG400.00527526514.2513513
CTTAAAC903.6541678E-813.7173483
CGCTTAC500.001496589613.30325518
ACTCCGT500.001500825413.2982676
TCGCTTA655.4484277E-513.1545999
GCGTAAC1207.385097E-1012.66786811
ATTCCGA1051.995977E-812.6650176
TTACAAT1600.012.47133416
CGTCAAT1850.012.32734119
CTAGCAC853.9578154E-612.2897513
GTTACAA1551.8189894E-1212.26060615
ACGGTAT2350.012.1283539
CGCGTAA1103.810601E-812.09114810
AGAGGTA550.00307277212.08842855
CCGATAA951.0386739E-612.0006889
CGCGGTC951.039145E-612.00023710
ACGAACG802.859572E-511.87746115
CCGTCAA1950.011.69516918
CGTCTTA1302.6047928E-911.69473115
Produced by FastQC (version 0.11.5)