##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064236_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334899
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18403564614252	32.0	32.0	32.0	32.0	32.0
2	30.791914594287658	32.0	32.0	32.0	32.0	32.0
3	30.80804015884348	32.0	32.0	32.0	32.0	32.0
4	30.833981447285524	32.0	32.0	32.0	32.0	32.0
5	30.74803786653522	32.0	32.0	32.0	32.0	32.0
6	34.37118463644066	36.0	36.0	36.0	32.0	36.0
7	34.316125789291924	36.0	36.0	36.0	32.0	36.0
8	34.2730753412805	36.0	36.0	36.0	32.0	36.0
9	34.40647494679373	36.0	36.0	36.0	32.0	36.0
10	34.11268642796197	36.0	36.0	36.0	32.0	36.0
11	34.385177455373025	36.0	36.0	36.0	32.0	36.0
12	34.2111762762576	36.0	36.0	36.0	32.0	36.0
13	34.28118381989948	36.0	36.0	36.0	32.0	36.0
14	34.18425663664442	36.0	36.0	36.0	32.0	36.0
15	34.120493011081734	36.0	36.0	36.0	32.0	36.0
16	34.12944125360795	36.0	36.0	36.0	32.0	36.0
17	34.05172301425052	36.0	36.0	36.0	32.0	36.0
18	34.04579297759606	36.0	36.0	36.0	32.0	36.0
19	34.06465432965341	36.0	36.0	36.0	32.0	36.0
20	34.04741182666254	36.0	36.0	36.0	32.0	36.0
21	34.01956852166344	36.0	36.0	36.0	32.0	36.0
22	34.00405199194846	36.0	36.0	36.0	32.0	36.0
23	33.95166525707188	36.0	36.0	36.0	32.0	36.0
24	33.94186901031464	36.0	36.0	36.0	32.0	36.0
25	33.525743895231024	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	16.0
5	18.0
6	54.0
7	28.0
8	81.0
9	76.0
10	123.0
11	45.0
12	79.0
13	56.0
14	116.0
15	225.0
16	340.0
17	464.0
18	604.0
19	793.0
20	1301.0
21	1843.0
22	2940.0
23	4903.0
24	7248.0
25	10907.0
26	15845.0
27	20754.0
28	28742.0
29	38761.0
30	51111.0
31	68998.0
32	96427.0
33	134508.0
34	277692.0
35	569794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.586029007315524	17.089271609730655	11.081876647717138	26.242822735236683
2	16.69768222389037	19.545344894494665	37.423191545788406	26.33378133582657
3	19.080855692530818	23.27039596305863	28.677655984993315	28.971092359417234
4	12.385276770376741	15.089415009855092	36.470056778212864	36.0552514415553
5	14.362292314873015	36.697176223972164	33.94292512659456	14.99760633456026
6	34.0040413303574	35.95173180071461	16.783508614986488	13.260718253941494
7	30.198234947676344	30.560222032732444	20.70137526618901	18.540167753402198
8	27.98247914808792	33.046215181241145	19.535075426593025	19.43623024407791
9	27.295634440306422	14.05685188335823	18.620127278546047	40.0273863977893
10	15.375096129485854	26.742061166086266	32.043386816056504	25.839455888371365
11	37.160926975113725	21.100233924878708	22.627453831503633	19.111385268503938
12	24.13984374355834	23.854105685602683	29.273909012067413	22.732141558771566
13	29.071287541358647	19.49534579352384	25.560356265301014	25.873010399816497
14	23.14021275271975	19.74562924416639	25.46541062954026	31.6487473735736
15	25.060938497839796	27.369405725970143	22.410601049971067	25.159054726218994
16	25.465519580644457	25.922325075503338	23.938287031866192	24.67386831198601
17	23.75601444891944	25.94456550174315	25.465058665941072	24.834361383396335
18	24.424589147542534	25.23081282850766	26.395991222552674	23.948606801397133
19	25.334972618195312	25.032929384555985	25.59773298898356	24.034365008265144
20	25.422651258544864	24.604713301875332	25.258988695812544	24.713646743767253
21	26.371013206347683	24.212836905804316	24.740536050570356	24.675613837277645
22	25.34459983236697	24.61559107136699	25.284698762844243	24.755110333421797
23	24.150503452567495	24.418386702554375	25.802862519587038	25.62824732529109
24	24.387034864324885	25.19864905895186	25.56869595204984	24.845620124673417
25	24.46917297291624	24.718224224095145	25.634360975873065	25.17824182711555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	266.0
3	448.0
4	448.0
5	448.0
6	1291.5
7	2135.0
8	2135.0
9	2135.0
10	2342.5
11	2550.0
12	2550.0
13	2550.0
14	3175.0
15	3800.0
16	3800.0
17	3800.0
18	6345.5
19	8891.0
20	8891.0
21	8891.0
22	14507.0
23	20123.0
24	20123.0
25	20123.0
26	29627.0
27	39131.0
28	39131.0
29	39131.0
30	49084.5
31	59038.0
32	59038.0
33	59038.0
34	72221.5
35	85405.0
36	85405.0
37	85405.0
38	98021.0
39	110637.0
40	110637.0
41	110637.0
42	126865.0
43	143093.0
44	143093.0
45	143093.0
46	158650.5
47	174208.0
48	174208.0
49	174208.0
50	178346.5
51	182485.0
52	182485.0
53	182485.0
54	168687.5
55	154890.0
56	154890.0
57	154890.0
58	141602.0
59	128314.0
60	128314.0
61	128314.0
62	112271.0
63	96228.0
64	96228.0
65	96228.0
66	78794.5
67	61361.0
68	61361.0
69	61361.0
70	45737.5
71	30114.0
72	30114.0
73	30114.0
74	22904.0
75	15694.0
76	15694.0
77	15694.0
78	12437.0
79	9180.0
80	9180.0
81	9180.0
82	6322.0
83	3464.0
84	3464.0
85	3464.0
86	2640.0
87	1816.0
88	1816.0
89	1816.0
90	1308.5
91	801.0
92	801.0
93	801.0
94	546.5
95	292.0
96	292.0
97	292.0
98	504.5
99	717.0
100	717.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035733040477219626
2	0.006367522936192176
3	0.002022624932672809
4	0.004195073934432493
5	0.009438916352473108
6	0.0142332865632531
7	0.024046763088443397
8	0.036631984891740875
9	0.04944194279866866
10	0.05700805828755584
11	0.053187544525840534
12	0.06067874798018427
13	0.06270137291285709
14	0.064574173776443
15	0.05745753049481646
16	0.06382505343100864
17	0.06142786832561865
18	0.07685974744156675
19	0.07371344199074237
20	0.0791820205124133
21	0.07453747437072018
22	0.0773841316833708
23	0.08307744630867205
24	0.0759608030270455
25	0.07768377982154455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1334899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.98186152229169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21883003370677	41.97161082803814
2	12.282943373687097	13.0154640982168
3	3.4380414209214067	5.464615034134827
4	1.5002694205934557	3.1794826675204493
5	0.7802654430922075	2.066995782827045
6	0.5033982470892879	1.6002585730709415
7	0.36596244713763915	1.3572560187623772
8	0.2576173787615338	1.091923862982348
9	0.20084075814945299	0.9576825512675753
>10	1.2582819629717383	12.385249463368778
>50	0.10017734881761411	3.695254681670134
>100	0.08356597332360433	8.955701164871767
>500	0.007674410933297258	2.755197794295568
>1k	0.0021317808148047937	1.5033074789732725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2700	0.2022624932672809	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1886	0.14128409714892287	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1486	0.11131928333154793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.491203454343737E-5
2	0.0	0.0	0.0	7.491203454343737E-5	7.491203454343737E-5
3	0.0	0.0	0.0	7.491203454343737E-5	7.491203454343737E-5
4	0.0	0.0	0.0	7.491203454343737E-5	7.491203454343737E-5
5	0.0	0.0	0.0	7.491203454343737E-5	1.4982406908687474E-4
6	0.0	0.0	0.0	7.491203454343737E-5	1.4982406908687474E-4
7	0.0	0.0	0.0	7.491203454343737E-5	1.4982406908687474E-4
8	0.0	0.0	0.0	7.491203454343737E-5	1.4982406908687474E-4
9	0.0	0.0	0.0	7.491203454343737E-5	1.4982406908687474E-4
10	0.0	0.0	0.0	2.247361036303121E-4	1.4982406908687474E-4
11	0.0	0.0	0.0	2.247361036303121E-4	1.4982406908687474E-4
12	0.0	0.0	0.0	2.247361036303121E-4	2.247361036303121E-4
13	7.491203454343737E-5	0.0	0.0	2.247361036303121E-4	5.99296276347499E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	50	8.691509E-5	15.20429	17
CTCGACG	40	0.005275265	14.25135	13
CTTAAAC	90	3.6541678E-8	13.717348	3
CGCTTAC	50	0.0014965896	13.303255	18
ACTCCGT	50	0.0015008254	13.298267	6
TCGCTTA	65	5.4484277E-5	13.154599	9
GCGTAAC	120	7.385097E-10	12.667868	11
ATTCCGA	105	1.995977E-8	12.665017	6
TTACAAT	160	0.0	12.471334	16
CGTCAAT	185	0.0	12.327341	19
CTAGCAC	85	3.9578154E-6	12.289751	3
GTTACAA	155	1.8189894E-12	12.260606	15
ACGGTAT	235	0.0	12.128353	9
CGCGTAA	110	3.810601E-8	12.091148	10
AGAGGTA	55	0.003072772	12.0884285	5
CCGATAA	95	1.0386739E-6	12.000688	9
CGCGGTC	95	1.039145E-6	12.000237	10
ACGAACG	80	2.859572E-5	11.877461	15
CCGTCAA	195	0.0	11.695169	18
CGTCTTA	130	2.6047928E-9	11.694731	15
>>END_MODULE