FastQCFastQC Report
Thu 2 Feb 2017
SRR4064236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334899
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13380.1002323022191192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2750.023.9970994
ACGTATG602.8709762E-422.00228532
GGTATAC654.934063E-420.3067591
GATATAC2750.019.999081
TGTAGGA4250.019.6674752
CGTCGTA3050.019.47451410
GTATCAA22100.019.3113291
AGGACGT3650.018.6826745
CCTTATA2250.018.5748372
ACCGTCG3200.018.5602558
TACCGTC3350.018.3858367
GATTTCG1203.1603668E-718.3345541
CCGTCGT3250.018.2767689
ATTTCGT1254.851936E-717.6005142
TATGGCG2900.017.44551316
ATATGGC5550.017.44064115
GTCCTAC3950.017.2650281
ATACCGT3700.017.241166
GGCGTTA3450.017.2178942
CGCAATA4100.017.17380136
Produced by FastQC (version 0.11.5)