##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334899
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297287659965285	32.0	32.0	32.0	32.0	32.0
2	31.32811920602233	32.0	32.0	32.0	32.0	32.0
3	31.424662839660527	32.0	32.0	32.0	32.0	32.0
4	31.509974162839285	32.0	32.0	32.0	32.0	32.0
5	31.461316549042287	32.0	32.0	32.0	32.0	32.0
6	35.024865551626	36.0	36.0	36.0	36.0	36.0
7	35.04172825060173	36.0	36.0	36.0	36.0	36.0
8	34.97635251805567	36.0	36.0	36.0	36.0	36.0
9	35.105253655894565	36.0	36.0	36.0	36.0	36.0
10	34.93702519816106	36.0	36.0	36.0	32.0	36.0
11	35.09986448412951	36.0	36.0	36.0	36.0	36.0
12	34.99887032651908	36.0	36.0	36.0	36.0	36.0
13	35.04482436498941	36.0	36.0	36.0	36.0	36.0
14	34.983980061412886	36.0	36.0	36.0	36.0	36.0
15	34.95320994322417	36.0	36.0	36.0	36.0	36.0
16	34.972293034903764	36.0	36.0	36.0	36.0	36.0
17	34.936995982467586	36.0	36.0	36.0	36.0	36.0
18	34.93420176357912	36.0	36.0	36.0	36.0	36.0
19	34.93740799865758	36.0	36.0	36.0	36.0	36.0
20	34.91995649109034	36.0	36.0	36.0	32.0	36.0
21	34.91652327254721	36.0	36.0	36.0	32.0	36.0
22	34.897497114013866	36.0	36.0	36.0	32.0	36.0
23	34.85159551396772	36.0	36.0	36.0	32.0	36.0
24	34.82482794578466	36.0	36.0	36.0	32.0	36.0
25	34.790659817709056	36.0	36.0	36.0	32.0	36.0
26	34.733149848790056	36.0	36.0	36.0	32.0	36.0
27	34.731038827656626	36.0	36.0	36.0	32.0	36.0
28	34.70660851495132	36.0	36.0	36.0	32.0	36.0
29	34.67730742176	36.0	36.0	36.0	32.0	36.0
30	34.665513271041476	36.0	36.0	36.0	32.0	36.0
31	34.648538953134285	36.0	36.0	36.0	32.0	36.0
32	34.61840783460022	36.0	36.0	36.0	32.0	36.0
33	34.601146603600725	36.0	36.0	36.0	32.0	36.0
34	34.58855538883466	36.0	36.0	36.0	32.0	36.0
35	34.550357742420964	36.0	36.0	36.0	32.0	36.0
36	34.52321037022276	36.0	36.0	36.0	32.0	36.0
37	34.51729231949383	36.0	36.0	36.0	32.0	36.0
38	34.47087757201107	36.0	36.0	36.0	32.0	36.0
39	34.475819518929896	36.0	36.0	36.0	32.0	36.0
40	34.45736643746081	36.0	36.0	36.0	32.0	36.0
41	34.443477746256455	36.0	36.0	36.0	32.0	36.0
42	34.380872260747815	36.0	36.0	36.0	32.0	36.0
43	34.37267238944669	36.0	36.0	36.0	32.0	36.0
44	34.30861136310687	36.0	36.0	36.0	32.0	36.0
45	34.283042387476506	36.0	36.0	36.0	32.0	36.0
46	34.257941612062034	36.0	36.0	36.0	32.0	36.0
47	34.23521180254087	36.0	36.0	36.0	32.0	36.0
48	34.20109836025048	36.0	36.0	36.0	32.0	36.0
49	34.175520395175965	36.0	36.0	36.0	32.0	36.0
50	33.56667508178521	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	14.0
21	42.0
22	120.0
23	401.0
24	1013.0
25	2400.0
26	4985.0
27	9231.0
28	15612.0
29	24601.0
30	35789.0
31	51324.0
32	74447.0
33	120507.0
34	245770.0
35	748640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4861304856615	18.168367048796895	11.731962439260473	26.61354002628113
2	16.041423513777122	20.42199605361924	36.82659447117149	26.709985961432142
3	18.140602818948306	24.04293970686833	29.0222826514445	28.794174822738867
4	12.037240270612234	15.90659667884986	36.52800698779458	35.52815606274332
5	14.077169883264576	37.048570715836924	33.62261864006191	15.251640760836588
6	34.10814908704635	36.40652694063517	16.381925810061894	13.10339816225659
7	30.105573530282065	30.77229063771866	20.650251442243945	18.47188438975533
8	28.050886246824664	33.34244763086945	19.221229471293334	19.38543665101255
9	27.458578890947322	13.830084494043316	18.44231009815812	40.26902651685124
10	15.41609608025866	27.003469964431364	31.993641395057548	25.586792560252427
11	37.54858989120509	21.064771289470276	22.462706860529313	18.923931958795322
12	24.194882305614364	23.922576856059415	29.040720714241836	22.841820124084386
13	29.266293803103466	19.470160177512223	25.554014026581882	25.709531992802425
14	23.234227060215133	19.782874633773243	25.24374539758498	31.739152908426643
15	25.11645573034112	27.45770660497647	22.2534951702149	25.172342494467507
16	25.5882333111342	26.135470642833436	23.73451835248976	24.5417776935426
17	23.908850032848928	26.09957757103721	25.243707576378437	24.747864819735426
18	24.599406394814867	25.27354231871594	26.27159909326134	23.85545219320785
19	25.582010324376604	24.991928228277946	25.449865495441976	23.976195951903478
20	25.567891958381683	24.579722869173786	25.141116179349204	24.711268993095324
21	26.55871343075394	24.277716890940813	24.588302186157904	24.575267492147347
22	25.467507980016435	24.69396515236756	25.172129385263432	24.66639748235257
23	24.17946115208426	24.448248350981544	25.59126816167685	25.78102233525734
24	24.596053766107097	25.256181581594927	25.315587091999138	24.83217756029884
25	24.707106529394977	24.759546053513784	25.47047617335308	25.06287124373816
26	24.500589964976683	25.329312831270016	25.75685951341936	24.41323769033394
27	25.14353909267711	25.06989664585999	25.034011872690716	24.752552388772187
28	24.33733928857711	25.07240535612348	25.77600711992245	24.814248235376965
29	24.442698122437484	25.011817639034245	25.574869144462863	24.97061509406541
30	24.411640215603942	25.405006536290458	25.826625363803558	24.35672788430204
31	24.973273289696564	24.866516282878347	25.040399098895943	25.119811328529146
32	24.669179781610712	25.14256133958216	24.8794633760443	25.308795502762827
33	24.37607034818998	24.74510168214161	25.47792029942031	25.400907670248102
34	24.962767965767505	24.91444872617	25.689279571374634	24.43350373668786
35	25.626897660965476	24.674742877005723	25.471900945936937	24.226458516091864
36	24.485320779255808	25.358926950012435	25.220180819471206	24.93557145126055
37	25.366725224837154	25.071636994280276	25.0024909072521	24.559146873630468
38	24.59898576793729	24.89423532910849	25.46061104147953	25.04616786147469
39	25.296682830864754	24.897684596615484	24.774449129972588	25.031183442547178
40	25.347786675856078	25.121697243926377	25.286657127660373	24.24385895255718
41	24.471648316413944	25.330690357558368	25.764593863471724	24.43306746255597
42	25.624156760040783	25.52647014977365	25.068751446758174	23.78062164342739
43	24.877105242261113	24.693044029359896	25.41408154428631	25.01576918409268
44	24.44498881175354	25.269780197964913	25.062795765621402	25.22243522466014
45	24.765579057371983	25.423238705955942	25.11497245081037	24.696209785861704
46	24.75245077107708	25.02123776861844	25.068582635520936	25.157728824783536
47	24.816180188737906	24.988631826241868	25.476171034267836	24.719016950752398
48	25.511417225210863	25.477556224618297	24.5636837772096	24.447342772961242
49	24.72765709439972	25.553562893943955	24.85013836263145	24.86864164902487
50	24.65594259854264	25.679256322108596	24.784343749508384	24.880457329840382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	16.5
2	17.0
3	74.5
4	132.0
5	162.5
6	193.0
7	186.5
8	180.0
9	246.0
10	312.0
11	475.5
12	639.0
13	1110.0
14	1581.0
15	2156.0
16	2731.0
17	2918.5
18	3106.0
19	3240.0
20	3374.0
21	4083.5
22	4793.0
23	5569.0
24	6345.0
25	7795.0
26	9245.0
27	11516.5
28	13788.0
29	15998.0
30	18208.0
31	20341.0
32	22474.0
33	25896.5
34	29319.0
35	33565.5
36	37812.0
37	44285.5
38	50759.0
39	55112.5
40	59466.0
41	64742.0
42	70018.0
43	71871.0
44	73724.0
45	81179.0
46	88634.0
47	93806.5
48	98979.0
49	102275.0
50	105571.0
51	103230.0
52	100889.0
53	98031.5
54	95174.0
55	94767.5
56	94361.0
57	91849.0
58	89337.0
59	81043.5
60	72750.0
61	63941.0
62	55132.0
63	47778.0
64	40424.0
65	34242.5
66	28061.0
67	23701.0
68	19341.0
69	17569.0
70	15797.0
71	11963.0
72	8129.0
73	6906.0
74	5683.0
75	4349.0
76	3015.0
77	2574.5
78	2134.0
79	1766.5
80	1399.0
81	1126.0
82	853.0
83	677.0
84	501.0
85	373.5
86	246.0
87	176.5
88	107.0
89	81.0
90	55.0
91	44.5
92	34.0
93	28.5
94	23.0
95	21.5
96	20.0
97	13.0
98	6.0
99	9.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007566115488887174
2	3.745601727171868E-4
3	2.996481381737495E-4
4	0.0
5	0.0
6	7.491203454343737E-5
7	0.0
8	0.0
9	8.989444145212483E-4
10	0.0011236805181515607
11	1.4982406908687474E-4
12	7.491203454343737E-5
13	3.745601727171868E-4
14	1.4982406908687474E-4
15	0.00426998596897593
16	8.989444145212483E-4
17	0.0
18	3.745601727171868E-4
19	0.0
20	4.4947220726062416E-4
21	0.0
22	1.4982406908687474E-4
23	0.0010487684836081232
24	4.4947220726062416E-4
25	0.0020975369672162464
26	0.005543490556214365
27	0.005318754452584053
28	0.0039703378308021805
29	0.0022473610363031213
30	0.003296129519911244
31	0.0068919071779962385
32	0.002621921209020308
33	0.0039703378308021805
34	0.002022624932672809
35	0.002397185105389996
36	0.006517347005279051
37	0.003296129519911244
38	0.0071915553161699865
39	0.004195073934432493
40	0.0021724490017596835
41	0.0029964813817374945
42	0.0013484166217818726
43	0.0012735045872384354
44	0.001198592552694998
45	0.0010487684836081232
46	9.738564490646859E-4
47	0.002397185105389996
48	0.0022473610363031213
49	7.491203454343737E-5
50	0.0014982406908687473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1334899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.076631678445786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9309702282675	44.82259577191371
2	12.00591225456666	13.465022389261467
3	3.3687764193110925	5.667289034182247
4	1.404567456528251	3.150536477090646
5	0.7484228863487202	2.0984517268748233
6	0.48064585573263463	1.6171800371814204
7	0.33295340532143286	1.3069633833405983
8	0.25374166997357434	1.1383182534865501
9	0.19235379843672037	0.9707897796197372
>10	1.1217057308272984	11.600325302616472
>50	0.0840076347759535	3.268044479651
>100	0.06841562055477729	7.6784513350173045
>500	0.006182925184799044	2.3358968930912356
>1k	0.001344114170608488	0.8801351366727874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1338	0.1002323022191192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.491203454343737E-5
2	0.0	0.0	0.0	0.0	7.491203454343737E-5
3	0.0	0.0	0.0	0.0	7.491203454343737E-5
4	0.0	0.0	0.0	0.0	7.491203454343737E-5
5	0.0	0.0	0.0	0.0	1.4982406908687474E-4
6	0.0	0.0	0.0	0.0	2.247361036303121E-4
7	0.0	0.0	0.0	0.0	2.247361036303121E-4
8	0.0	0.0	0.0	0.0	2.247361036303121E-4
9	0.0	0.0	0.0	0.0	2.247361036303121E-4
10	0.0	0.0	0.0	7.491203454343737E-5	2.247361036303121E-4
11	0.0	0.0	0.0	7.491203454343737E-5	2.996481381737495E-4
12	0.0	0.0	0.0	7.491203454343737E-5	3.745601727171868E-4
13	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
14	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
15	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
16	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
17	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
18	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
19	0.0	0.0	0.0	7.491203454343737E-5	4.494722072606242E-4
20	0.0	0.0	0.0	1.4982406908687474E-4	4.494722072606242E-4
21	0.0	0.0	0.0	1.4982406908687474E-4	5.99296276347499E-4
22	0.0	0.0	0.0	2.247361036303121E-4	5.99296276347499E-4
23	0.0	0.0	0.0	5.243842418040616E-4	5.99296276347499E-4
24	0.0	0.0	0.0	7.491203454343736E-4	5.99296276347499E-4
25	0.0	0.0	0.0	9.738564490646858E-4	5.99296276347499E-4
26	0.0	0.0	0.0	0.0012735045872384354	7.491203454343736E-4
27	0.0	0.0	0.0	0.0016480647599556222	8.240323799778111E-4
28	0.0	0.0	0.0	0.003221217485367807	8.240323799778111E-4
29	0.0	0.0	0.0	0.007865763627060924	8.240323799778111E-4
30	0.0	0.0	0.0	0.01513223097777435	9.738564490646858E-4
31	0.0	0.0	0.0	0.02996481381737495	0.0011236805181515605
32	0.0	0.0	0.0	0.046520373451474605	0.0011236805181515605
33	0.0	0.0	0.0	0.05985471560020646	0.0011236805181515605
34	0.0	0.0	0.0	0.07828307609789205	0.001198592552694998
35	0.0	0.0	0.0	0.09633687642286046	0.001198592552694998
36	0.0	0.0	0.0	0.12585221803297478	0.001198592552694998
37	0.0	0.0	0.0	0.17064961468995032	0.001198592552694998
38	0.0	0.0	0.0	0.23702167729543583	0.001198592552694998
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	275	0.0	23.997099	4
ACGTATG	60	2.8709762E-4	22.002285	32
GGTATAC	65	4.934063E-4	20.306759	1
GATATAC	275	0.0	19.99908	1
TGTAGGA	425	0.0	19.667475	2
CGTCGTA	305	0.0	19.474514	10
GTATCAA	2210	0.0	19.311329	1
AGGACGT	365	0.0	18.682674	5
CCTTATA	225	0.0	18.574837	2
ACCGTCG	320	0.0	18.560255	8
TACCGTC	335	0.0	18.385836	7
GATTTCG	120	3.1603668E-7	18.33455	41
CCGTCGT	325	0.0	18.276768	9
ATTTCGT	125	4.851936E-7	17.60051	42
TATGGCG	290	0.0	17.445513	16
ATATGGC	555	0.0	17.440641	15
GTCCTAC	395	0.0	17.265028	1
ATACCGT	370	0.0	17.24116	6
GGCGTTA	345	0.0	17.21789	42
CGCAATA	410	0.0	17.173801	36
>>END_MODULE