FastQCFastQC Report
Thu 2 Feb 2017
SRR4064235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948358
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53400.2740769406854387No Hit
TATCAACGCAGAGTACTTTTTTTTT33780.17337676135494606No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21930.1125563166522785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA402.7581226E-416.6277213
TTTTCGG1551.8189894E-1212.25975516
GATTAGG550.00307994212.0851251
ATACCGT2600.011.6915176
CGAGCCG2300.011.5671115
AACCGAC752.0726616E-411.4009877
CGTCGTA2500.011.40069310
TTTAGAA4750.011.3939622
ACTGTTC5200.011.1454818
GATATAC4700.011.1117041
GTATCAA31300.011.10331251
TTAGGAC5600.011.0229263
CCGTCGT2500.011.0209549
CGCGTAA951.3596558E-511.00066910
GCGTCCC1303.2605385E-810.9630516
GTCCTAC8950.010.9277311
ACCGTCG2350.010.9161188
CGGTCCA2350.010.91555710
CGTTTTG700.001489660510.8589215
TAGAAAT4200.010.8555744
Produced by FastQC (version 0.11.5)