##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064235_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1948358
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21218431109683	32.0	32.0	32.0	32.0	32.0
2	30.795104903718926	32.0	32.0	32.0	32.0	32.0
3	30.814755296511215	32.0	32.0	32.0	32.0	32.0
4	30.84337272718874	32.0	32.0	32.0	32.0	32.0
5	30.756429259920406	32.0	32.0	32.0	32.0	32.0
6	34.40080005830551	36.0	36.0	36.0	32.0	36.0
7	34.338246872494686	36.0	36.0	36.0	32.0	36.0
8	34.307880276622676	36.0	36.0	36.0	32.0	36.0
9	34.43807503549142	36.0	36.0	36.0	32.0	36.0
10	34.1326167983502	36.0	36.0	36.0	32.0	36.0
11	34.41290255692229	36.0	36.0	36.0	32.0	36.0
12	34.23197533512835	36.0	36.0	36.0	32.0	36.0
13	34.309856812762334	36.0	36.0	36.0	32.0	36.0
14	34.21819911946367	36.0	36.0	36.0	32.0	36.0
15	34.14276739695682	36.0	36.0	36.0	32.0	36.0
16	34.150011958787864	36.0	36.0	36.0	32.0	36.0
17	34.082098361800035	36.0	36.0	36.0	32.0	36.0
18	34.074346193050765	36.0	36.0	36.0	32.0	36.0
19	34.0826747445798	36.0	36.0	36.0	32.0	36.0
20	34.05922217580137	36.0	36.0	36.0	32.0	36.0
21	34.051498749203176	36.0	36.0	36.0	32.0	36.0
22	34.02029247191738	36.0	36.0	36.0	32.0	36.0
23	33.96282869985906	36.0	36.0	36.0	32.0	36.0
24	33.9468100831572	36.0	36.0	36.0	32.0	36.0
25	33.5458113960576	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	18.0
5	23.0
6	81.0
7	25.0
8	113.0
9	134.0
10	184.0
11	57.0
12	121.0
13	92.0
14	258.0
15	347.0
16	609.0
17	803.0
18	1030.0
19	1466.0
20	2313.0
21	3573.0
22	5426.0
23	8197.0
24	11907.0
25	16681.0
26	23355.0
27	30087.0
28	40513.0
29	53494.0
30	69872.0
31	94542.0
32	131324.0
33	186011.0
34	397673.0
35	868020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.267054224161036	17.208055938081166	11.478387046091	26.046502791666793
2	16.323069650318924	19.863611702949452	38.19113558193692	25.622183064794708
3	19.05296919758089	24.09638863411111	28.482216226176284	28.368425942131715
4	12.74780832323896	15.678598558728723	35.79844786170366	35.77514525632866
5	14.552872195008776	36.66367412850667	33.64352043626381	15.13993324022074
6	33.66350101971099	35.42793725787318	17.228829511406236	13.679732211009593
7	29.70902964018613	30.4170756549631	20.982331705594138	18.891562999256635
8	27.54302795861019	33.53575039188675	19.63176765184745	19.289453997655613
9	27.555452853199817	14.31439758649464	18.491815906027345	39.6383336542782
10	15.97404891736964	26.945432471823672	31.854476320381757	25.226042290424928
11	36.81269302625106	21.080495092113384	22.94474223966427	19.16206964197129
12	24.970468477796402	23.335192535252933	29.281865282845214	22.412473704105444
13	29.807463164901264	19.836101272896105	25.11083399758404	25.245601564618582
14	23.476968796433876	19.835859370546007	25.30681991526403	31.380351917756084
15	24.811378264962503	27.754087895597205	22.33124190162961	25.103291937810678
16	24.984065334951538	25.806299187524683	24.572203678146987	24.637431799376788
17	23.683809114893762	25.98659426412001	25.591026959899377	24.73856966108685
18	24.28239458974678	25.161981937742638	26.79623509493934	23.75938837757124
19	25.57109264968293	24.66420666481071	25.440165351572414	24.32453533393395
20	25.810125138817693	24.732509484805306	25.180732669747968	24.27663270662904
21	26.315978714224077	24.27903679809332	24.63402231308171	24.770962174600893
22	25.720665374982275	24.78215667460443	25.03092234894792	24.46625560146537
23	24.492991259107665	24.637183106694778	25.552726854306133	25.317098779891428
24	24.79449894623405	24.97823381327668	25.609928360352512	24.617338880136757
25	24.759918144106642	24.845133334292154	25.637187724081535	24.75776079751967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	485.0
3	762.0
4	762.0
5	762.0
6	1970.5
7	3179.0
8	3179.0
9	3179.0
10	3534.0
11	3889.0
12	3889.0
13	3889.0
14	4497.0
15	5105.0
16	5105.0
17	5105.0
18	8848.0
19	12591.0
20	12591.0
21	12591.0
22	20675.5
23	28760.0
24	28760.0
25	28760.0
26	43552.5
27	58345.0
28	58345.0
29	58345.0
30	76274.5
31	94204.0
32	94204.0
33	94204.0
34	111208.0
35	128212.0
36	128212.0
37	128212.0
38	144790.0
39	161368.0
40	161368.0
41	161368.0
42	184043.0
43	206718.0
44	206718.0
45	206718.0
46	228347.5
47	249977.0
48	249977.0
49	249977.0
50	256024.0
51	262071.0
52	262071.0
53	262071.0
54	244146.0
55	226221.0
56	226221.0
57	226221.0
58	207143.5
59	188066.0
60	188066.0
61	188066.0
62	164035.5
63	140005.0
64	140005.0
65	140005.0
66	114185.0
67	88365.0
68	88365.0
69	88365.0
70	66768.0
71	45171.0
72	45171.0
73	45171.0
74	33904.0
75	22637.0
76	22637.0
77	22637.0
78	17657.0
79	12677.0
80	12677.0
81	12677.0
82	8735.0
83	4793.0
84	4793.0
85	4793.0
86	3705.0
87	2617.0
88	2617.0
89	2617.0
90	1819.0
91	1021.0
92	1021.0
93	1021.0
94	716.0
95	411.0
96	411.0
97	411.0
98	698.0
99	985.0
100	985.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03982840935803379
2	0.004978551169754224
3	0.0018990349822773844
4	0.004003371043719891
5	0.009187223292639239
6	0.01319059433635913
7	0.023712274643571663
8	0.0374161216778436
9	0.05045274020482888
10	0.05933201187872044
11	0.05563659245374823
12	0.06600429695158692
13	0.06682550126824742
14	0.07026429434426322
15	0.06194960063807575
16	0.06903248786927249
17	0.06574767060263052
18	0.07883561439940709
19	0.07575609821193026
20	0.08006742087439783
21	0.07791175954316404
22	0.07934886709731989
23	0.08448139407644796
24	0.07857898805045069
25	0.07811706062232916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1948358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.45804836548699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23809987319135	41.28998078679378
2	13.188593832043988	14.100729738923418
3	3.961271960083436	6.352856040929633
4	1.7458497252163159	3.733188761979439
5	0.9590631853365092	2.563482307363856
6	0.6067357392265024	1.946094509558392
7	0.42152474123941397	1.5773723003098188
8	0.3038636766997717	1.2995167300424877
9	0.22930528171938164	1.103239155655469
>10	1.1937005223788513	11.418287975483462
>50	0.07752698641584281	2.850633740275803
>100	0.06510941500926444	7.241724709912304
>500	0.0070404070626697455	2.578522442044146
>1k	0.0022182104444027962	1.669056185717616
>5k	9.644393236533896E-5	0.27531461501041204
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5340	0.2740769406854387	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3378	0.17337676135494606	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2193	0.1125563166522785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0265053958256132E-4
2	0.0	0.0	0.0	0.0	1.0265053958256132E-4
3	0.0	0.0	0.0	0.0	1.0265053958256132E-4
4	0.0	0.0	0.0	0.0	1.0265053958256132E-4
5	0.0	0.0	0.0	0.0	1.0265053958256132E-4
6	0.0	0.0	0.0	0.0	1.0265053958256132E-4
7	0.0	0.0	0.0	0.0	1.0265053958256132E-4
8	0.0	0.0	0.0	0.0	1.0265053958256132E-4
9	0.0	0.0	0.0	0.0	1.0265053958256132E-4
10	0.0	0.0	0.0	0.0	1.0265053958256132E-4
11	0.0	0.0	0.0	0.0	1.0265053958256132E-4
12	0.0	0.0	0.0	0.0	2.566263489564033E-4
13	0.0	0.0	0.0	0.0	3.0795161874768394E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	40	2.7581226E-4	16.62772	13
TTTTCGG	155	1.8189894E-12	12.259755	16
GATTAGG	55	0.003079942	12.085125	1
ATACCGT	260	0.0	11.691517	6
CGAGCCG	230	0.0	11.56711	15
AACCGAC	75	2.0726616E-4	11.400987	7
CGTCGTA	250	0.0	11.400693	10
TTTAGAA	475	0.0	11.393962	2
ACTGTTC	520	0.0	11.145481	8
GATATAC	470	0.0	11.111704	1
GTATCAA	3130	0.0	11.1033125	1
TTAGGAC	560	0.0	11.022926	3
CCGTCGT	250	0.0	11.020954	9
CGCGTAA	95	1.3596558E-5	11.000669	10
GCGTCCC	130	3.2605385E-8	10.96305	16
GTCCTAC	895	0.0	10.927731	1
ACCGTCG	235	0.0	10.916118	8
CGGTCCA	235	0.0	10.915557	10
CGTTTTG	70	0.0014896605	10.85892	15
TAGAAAT	420	0.0	10.855574	4
>>END_MODULE