FastQCFastQC Report
Thu 2 Feb 2017
SRR4064235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948358
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36800.020.9238641
TCTAGCG4100.019.3180228
GTACGTA600.00741598418.3323511
ATACGAA4250.018.1180740
TAGGACG10450.018.1038274
TACGAAT4400.018.0004241
GTCCTAA6000.017.9666231
CGCAATA4350.017.7033836
TTAGGAC7150.017.537073
TACACCG1651.6625563E-917.3319595
CTAGCGG4500.017.11151129
AGTACGT902.2175908E-417.1097555
GTGTATA6850.017.0218581
CGAATGC4350.016.68961743
GGACGTG11250.016.4253646
GCGCAAT4700.016.38504235
AGGACGT11550.016.3796545
GTAGGAC12100.016.3623393
ATAGCGT3400.016.1751886
TCCAATA5050.016.1175332
Produced by FastQC (version 0.11.5)