##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1948358
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290334219891825	32.0	32.0	32.0	32.0	32.0
2	31.392882108934806	32.0	32.0	32.0	32.0	32.0
3	31.46303554069632	32.0	32.0	32.0	32.0	32.0
4	31.548429498069655	32.0	32.0	32.0	32.0	32.0
5	31.499130036677037	32.0	32.0	32.0	32.0	32.0
6	35.07434003401839	36.0	36.0	36.0	36.0	36.0
7	35.095085194815326	36.0	36.0	36.0	36.0	36.0
8	35.04539617462499	36.0	36.0	36.0	36.0	36.0
9	35.14190667218242	36.0	36.0	36.0	36.0	36.0
10	35.010354359927696	36.0	36.0	36.0	36.0	36.0
11	35.144407752579355	36.0	36.0	36.0	36.0	36.0
12	35.05802578376253	36.0	36.0	36.0	36.0	36.0
13	35.09653256742344	36.0	36.0	36.0	36.0	36.0
14	35.037476685496195	36.0	36.0	36.0	36.0	36.0
15	35.01098514749343	36.0	36.0	36.0	36.0	36.0
16	35.0254029290305	36.0	36.0	36.0	36.0	36.0
17	34.99192396879834	36.0	36.0	36.0	36.0	36.0
18	34.98948961125214	36.0	36.0	36.0	36.0	36.0
19	34.980525139630394	36.0	36.0	36.0	36.0	36.0
20	34.97701500442937	36.0	36.0	36.0	36.0	36.0
21	34.97256920956005	36.0	36.0	36.0	36.0	36.0
22	34.950463929113646	36.0	36.0	36.0	36.0	36.0
23	34.90731580130551	36.0	36.0	36.0	32.0	36.0
24	34.873799373626404	36.0	36.0	36.0	32.0	36.0
25	34.844360738632226	36.0	36.0	36.0	32.0	36.0
26	34.78793835629797	36.0	36.0	36.0	32.0	36.0
27	34.7732993628481	36.0	36.0	36.0	32.0	36.0
28	34.753433917175386	36.0	36.0	36.0	32.0	36.0
29	34.72806948209723	36.0	36.0	36.0	32.0	36.0
30	34.70653391214551	36.0	36.0	36.0	32.0	36.0
31	34.691240008253104	36.0	36.0	36.0	32.0	36.0
32	34.651629218038984	36.0	36.0	36.0	32.0	36.0
33	34.631672926638736	36.0	36.0	36.0	32.0	36.0
34	34.62115381259502	36.0	36.0	36.0	32.0	36.0
35	34.58806133164439	36.0	36.0	36.0	32.0	36.0
36	34.55318273130503	36.0	36.0	36.0	32.0	36.0
37	34.53413797669627	36.0	36.0	36.0	32.0	36.0
38	34.499313267890194	36.0	36.0	36.0	32.0	36.0
39	34.4846075515896	36.0	36.0	36.0	32.0	36.0
40	34.47367218960787	36.0	36.0	36.0	32.0	36.0
41	34.45262369646646	36.0	36.0	36.0	32.0	36.0
42	34.39577788065643	36.0	36.0	36.0	32.0	36.0
43	34.3791377149374	36.0	36.0	36.0	32.0	36.0
44	34.31262735082567	36.0	36.0	36.0	32.0	36.0
45	34.275861520316084	36.0	36.0	36.0	32.0	36.0
46	34.24213927830512	36.0	36.0	36.0	32.0	36.0
47	34.20720575992708	36.0	36.0	36.0	32.0	36.0
48	34.15927103745821	36.0	36.0	36.0	32.0	36.0
49	34.13912997508671	36.0	36.0	36.0	32.0	36.0
50	33.54318970127667	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	16.0
20	32.0
21	113.0
22	292.0
23	791.0
24	1931.0
25	4166.0
26	8198.0
27	14603.0
28	23999.0
29	36012.0
30	50986.0
31	71136.0
32	101058.0
33	160282.0
34	331889.0
35	1142850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42745127025338	18.090908810929506	12.010200216200234	26.471439702616884
2	15.653185693953503	20.622208216076057	37.58829902825517	26.136307061715264
3	18.357238982238343	24.628224645008043	28.774853029788222	28.239683342965392
4	12.426566370246125	16.366602031043577	35.88088020784681	35.32595139086349
5	14.093354506717965	37.10750283058863	33.51062792361568	15.288514739077726
6	33.807048708219284	35.7197885192498	16.93647519422775	13.536687578303155
7	29.656212372084333	30.516220358312708	21.0786130241219	18.74895424548106
8	27.570138547433277	33.78783570575839	19.481430004136815	19.16059574267152
9	27.643822876365142	14.094862585162504	18.40516051149341	39.85615402697895
10	15.961409259047304	27.207119075029397	31.85055198186356	24.98091968405974
11	37.01277537946074	21.043371414992222	22.86973075262901	19.074122452918022
12	24.919778520968446	23.356563174286425	29.334834085762562	22.388824218982567
13	29.930484697805525	19.869037769433387	25.096543327988634	25.103934204772454
14	23.52126613411512	19.677256107200122	25.37221123860321	31.429266520081544
15	24.894008639450753	27.835589691788414	22.358633702070346	24.911767966690483
16	25.192163190894423	25.77645426064391	24.498137905831825	24.533244642629843
17	23.767141356978545	26.05193706700719	25.437111660177443	24.743809915836824
18	24.412029635256406	25.098300874327318	26.781105086085443	23.708564404330833
19	25.714627393938894	24.599893859342075	25.569890133127487	24.115588613591548
20	25.9855815868056	24.62760644691743	25.27990465739588	24.10690730888109
21	26.511862809606857	24.24744323168535	24.65912322068121	24.581570738026585
22	25.798314065807276	24.71914783540585	25.10916896090858	24.373369137878292
23	24.573060725898134	24.526149116301273	25.630881594378817	25.26990856342178
24	24.972925809017887	24.84225113557626	25.64267200451664	24.542151050889213
25	24.982189820584257	24.618697916773595	25.739969327483518	24.659142935158627
26	24.26195227712283	25.323932066143527	26.129373673166867	24.284741983566775
27	24.82514862238775	25.067882268278936	25.446431779475276	24.660537329858034
28	24.37363628944463	25.127998238436273	25.651334594966098	24.847030877153
29	24.505865354757084	24.84877881029921	25.432619636042613	25.212736198901098
30	24.320463671233608	25.241841894145445	25.69229118857329	24.745403246047662
31	25.051239106075858	24.75589243303756	25.137215066683982	25.055653394202604
32	24.611929121471075	25.264552369230266	25.044513370949208	25.07900513834946
33	24.339886350046168	24.91059985227867	25.675127023404954	25.07438677427021
34	24.958823097663043	24.9258713104392	25.767065766531967	24.348239825365795
35	25.18564978966192	24.970332989100196	25.732077121909857	24.111940099328027
36	24.318547658547	25.334925921059114	25.476544406300818	24.86998201409307
37	24.943552861673588	25.13207783468829	25.154097223042033	24.770272080596094
38	24.596613788660786	25.264668064541794	25.360654349282285	24.778063797515134
39	25.246718504028955	24.63299547855973	25.18122460250694	24.93906141490438
40	25.579761248512806	24.903274748986554	25.503541032139783	24.013422970360857
41	24.626621293544595	24.925036827166387	26.02507840208593	24.423263477203086
42	25.263021744910226	25.60085160635995	25.391388880049355	23.744737768680473
43	24.541299942720237	24.915209522812333	25.714662593438902	24.828827941028532
44	24.18142885152135	25.43573124525877	25.2823694500019	25.100470453217984
45	24.403925413046046	25.368443744129586	25.402729517073595	24.82490132575077
46	24.409753944383425	24.81615118511143	25.357586458215714	25.416508412289435
47	24.85432298748099	24.976479700171993	25.53347324896308	24.635724063383936
48	24.977724398456104	25.304980701322165	24.78314650570748	24.93414839451425
49	24.577334233925356	25.459106894661527	25.038699452048608	24.92485941936451
50	24.493580416098872	25.893647652996023	24.79368281122086	24.819089119684243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	49.0
2	46.0
3	155.0
4	264.0
5	321.0
6	378.0
7	368.0
8	358.0
9	472.5
10	587.0
11	898.0
12	1209.0
13	2199.0
14	3189.0
15	4230.5
16	5272.0
17	5602.0
18	5932.0
19	5991.5
20	6051.0
21	6556.0
22	7061.0
23	8064.0
24	9067.0
25	10510.0
26	11953.0
27	15591.0
28	19229.0
29	23091.5
30	26954.0
31	31257.5
32	35561.0
33	42279.0
34	48997.0
35	56896.0
36	64795.0
37	72075.5
38	79356.0
39	83275.0
40	87194.0
41	92483.0
42	97772.0
43	100850.5
44	103929.0
45	112894.5
46	121860.0
47	129169.0
48	136478.0
49	141698.5
50	146919.0
51	145389.0
52	143859.0
53	142379.5
54	140900.0
55	139572.5
56	138245.0
57	133866.0
58	129487.0
59	118900.5
60	108314.0
61	95122.5
62	81931.0
63	70938.5
64	59946.0
65	50780.0
66	41614.0
67	35079.5
68	28545.0
69	24895.0
70	21245.0
71	16529.5
72	11814.0
73	10123.0
74	8432.0
75	6637.0
76	4842.0
77	4179.5
78	3517.0
79	2887.5
80	2258.0
81	1800.0
82	1342.0
83	1078.0
84	814.0
85	595.0
86	376.0
87	270.5
88	165.0
89	119.5
90	74.0
91	67.0
92	60.0
93	50.0
94	40.0
95	36.5
96	33.0
97	27.5
98	22.0
99	21.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008417344245770028
2	3.592768885389646E-4
3	2.0530107916512264E-4
4	0.0
5	0.0
6	5.132526979128066E-5
7	2.0530107916512264E-4
8	0.0
9	0.0012318064749907358
10	7.698790468692099E-4
11	5.132526979128066E-5
12	1.0265053958256132E-4
13	5.132526979128066E-4
14	1.0265053958256132E-4
15	0.004413973202050136
16	7.185537770779292E-4
17	0.0
18	1.5397580937384197E-4
19	0.0
20	8.212043166604905E-4
21	0.0
22	1.0265053958256132E-4
23	6.672285072866485E-4
24	4.106021583302453E-4
25	0.0017450591729035424
26	0.005645779677040872
27	0.006774935612449047
28	0.004567949011423978
29	0.002720239298937875
30	0.004157346853093733
31	0.0071342125009880115
32	0.003438793076015804
33	0.004875900630171663
34	0.0029255403781029976
35	0.0033874678062245237
36	0.008519994785352588
37	0.0038493952343460493
38	0.008366018975978747
39	0.004362647932258856
40	0.0028742151083117166
41	0.0034901183458070847
42	0.0020530107916512264
43	0.001334457014573297
44	0.0012318064749907358
45	0.0014371075541558583
46	9.238548562430518E-4
47	0.002104336061442507
48	0.001950360252068665
49	5.132526979128066E-4
50	0.0011804812051994552
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1948358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61054374568023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36752459810839	45.147957040999955
2	12.831375507397885	14.784450399723918
3	3.8203043986856637	6.602694410468852
4	1.6042268080709885	3.6968151481746663
5	0.8582998190278677	2.47235596355072
6	0.5338940144933365	1.8454754686515122
7	0.35783437310420074	1.4430522963799335
8	0.2664721613194908	1.228128488536199
9	0.1926126298790942	0.9986866505658066
>10	1.046720435919815	10.56224719833703
>50	0.06371585000884478	2.5260361318098963
>100	0.05038640429910421	5.826585167320542
>500	0.005467742983913696	2.067172269448058
>1k	0.0011652567014898026	0.7983433660329982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.132526979128066E-5	0.0
5	0.0	0.0	0.0	5.132526979128066E-5	0.0
6	0.0	0.0	0.0	5.132526979128066E-5	0.0
7	0.0	0.0	0.0	5.132526979128066E-5	0.0
8	0.0	0.0	0.0	1.0265053958256132E-4	0.0
9	0.0	0.0	0.0	1.0265053958256132E-4	0.0
10	0.0	0.0	0.0	1.0265053958256132E-4	0.0
11	0.0	0.0	0.0	1.0265053958256132E-4	0.0
12	0.0	0.0	0.0	1.0265053958256132E-4	1.5397580937384197E-4
13	0.0	0.0	0.0	1.0265053958256132E-4	2.0530107916512264E-4
14	0.0	0.0	0.0	1.0265053958256132E-4	2.0530107916512264E-4
15	0.0	0.0	0.0	1.0265053958256132E-4	3.0795161874768394E-4
16	0.0	0.0	0.0	1.0265053958256132E-4	3.592768885389646E-4
17	0.0	0.0	0.0	1.5397580937384197E-4	3.592768885389646E-4
18	0.0	0.0	0.0	2.566263489564033E-4	3.592768885389646E-4
19	0.0	0.0	0.0	2.566263489564033E-4	3.592768885389646E-4
20	0.0	0.0	0.0	3.592768885389646E-4	4.619274281215259E-4
21	0.0	0.0	0.0	4.106021583302453E-4	5.132526979128066E-4
22	0.0	0.0	0.0	6.672285072866485E-4	5.132526979128066E-4
23	0.0	0.0	0.0	0.0011291559354081745	5.132526979128066E-4
24	0.0	0.0	0.0	0.0018990349822773844	5.132526979128066E-4
25	0.0	0.0	0.0	0.0026175887593553136	5.132526979128066E-4
26	0.0	0.0	0.0	0.003284817266641962	5.645779677040872E-4
27	0.0	0.0	0.0	0.004003371043719891	6.159032374953679E-4
28	0.0	0.0	0.0	0.0069802366916141695	6.159032374953679E-4
29	0.0	0.0	0.0	0.014576376620723707	6.159032374953679E-4
30	0.0	0.0	0.0	0.02730504352896131	6.159032374953679E-4
31	0.0	0.0	0.0	0.06117972159120654	6.159032374953679E-4
32	0.0	0.0	0.0	0.09510572492324305	7.185537770779292E-4
33	0.0	0.0	0.0	0.12913437879486214	7.185537770779292E-4
34	0.0	0.0	0.0	0.16629387412374932	7.698790468692099E-4
35	0.0	0.0	0.0	0.2072514394171913	7.698790468692099E-4
36	0.0	0.0	0.0	0.2675073061521548	7.698790468692099E-4
37	0.0	0.0	0.0	0.35034629159528174	7.698790468692099E-4
38	0.0	0.0	0.0	0.4632618851360992	7.698790468692099E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3680	0.0	20.923864	1
TCTAGCG	410	0.0	19.31802	28
GTACGTA	60	0.007415984	18.33235	11
ATACGAA	425	0.0	18.11807	40
TAGGACG	1045	0.0	18.103827	4
TACGAAT	440	0.0	18.00042	41
GTCCTAA	600	0.0	17.966623	1
CGCAATA	435	0.0	17.70338	36
TTAGGAC	715	0.0	17.53707	3
TACACCG	165	1.6625563E-9	17.331959	5
CTAGCGG	450	0.0	17.111511	29
AGTACGT	90	2.2175908E-4	17.109755	5
GTGTATA	685	0.0	17.021858	1
CGAATGC	435	0.0	16.689617	43
GGACGTG	1125	0.0	16.425364	6
GCGCAAT	470	0.0	16.385042	35
AGGACGT	1155	0.0	16.379654	5
GTAGGAC	1210	0.0	16.362339	3
ATAGCGT	340	0.0	16.175188	6
TCCAATA	505	0.0	16.117533	2
>>END_MODULE