FastQCFastQC Report
Thu 2 Feb 2017
SRR4064234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121217
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31050.2769312274073618No Hit
TATCAACGCAGAGTACTTTTTTTTT21180.18890188072424874No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16130.14386153617007236No Hit
GCGCAAGACGGACCAGAGCGAAAGC13810.12316973431548042No Hit
GTATCTGATCGTCTTCGAACCTCCG12070.10765088292453645No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11960.10666980611246529No Hit
GATTAAGAGGGACGGCCGGGGGCAT11580.10328063167076489No Hit
GAATAGGACCGCGGTTCTATTTTGT11450.10212117725649895No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11420.10185361085320684No Hit
GAACTACGACGGTATCTGATCGTCT11420.10185361085320684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC601.4722864E-615.8267522
AAAGACG400.005281506614.2485285
GCGTTAT1051.375156E-913.5676041
TCCTAAA1205.2750693E-1113.4527392
CCGTGCG500.001497738613.3015938
TGCGTAC500.001498242613.30111
CGTTATT1001.0119038E-813.2944712
GTATAAT1153.783498E-1013.2136671
GTCCTAA1301.4551915E-1113.1501391
CACGCCC957.3678166E-813.00155716
CGTCTTA604.0863067E-412.66818515
CCGTTAA604.0863067E-412.66818516
TTAGAAC604.1017233E-412.6625313
TTTATAC604.1017233E-412.6625313
CGACCAT1600.012.47024410
GCGCCCC1001.438766E-712.3514815
ACGGTAT2700.011.9638629
TCCAACG802.859868E-511.87695318
CGGTGAG1052.7128226E-711.76331515
GGTCGCG1302.6066118E-911.6937097