##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064234_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1121217
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19135814030647	32.0	32.0	32.0	32.0	32.0
2	30.80632651841704	32.0	32.0	32.0	32.0	32.0
3	30.83110049169786	32.0	32.0	32.0	32.0	32.0
4	30.850428596783672	32.0	32.0	32.0	32.0	32.0
5	30.779540445783464	32.0	32.0	32.0	32.0	32.0
6	34.409699460496945	36.0	36.0	36.0	32.0	36.0
7	34.32819249083808	36.0	36.0	36.0	32.0	36.0
8	34.2881583136895	36.0	36.0	36.0	32.0	36.0
9	34.414434493947205	36.0	36.0	36.0	32.0	36.0
10	34.139708013702965	36.0	36.0	36.0	32.0	36.0
11	34.395510414130364	36.0	36.0	36.0	32.0	36.0
12	34.21350550339497	36.0	36.0	36.0	32.0	36.0
13	34.295620740677315	36.0	36.0	36.0	32.0	36.0
14	34.20847079557303	36.0	36.0	36.0	32.0	36.0
15	34.13907031377512	36.0	36.0	36.0	32.0	36.0
16	34.1394912849163	36.0	36.0	36.0	32.0	36.0
17	34.06730632874814	36.0	36.0	36.0	32.0	36.0
18	34.05846415100734	36.0	36.0	36.0	32.0	36.0
19	34.07685220612959	36.0	36.0	36.0	32.0	36.0
20	34.04944983888043	36.0	36.0	36.0	32.0	36.0
21	34.03309172087116	36.0	36.0	36.0	32.0	36.0
22	34.01855662195632	36.0	36.0	36.0	32.0	36.0
23	33.95812407410876	36.0	36.0	36.0	32.0	36.0
24	33.949573543747555	36.0	36.0	36.0	32.0	36.0
25	33.55955626787678	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	12.0
6	36.0
7	17.0
8	76.0
9	68.0
10	97.0
11	25.0
12	73.0
13	70.0
14	128.0
15	226.0
16	364.0
17	426.0
18	579.0
19	848.0
20	1235.0
21	1822.0
22	2824.0
23	4378.0
24	6562.0
25	9496.0
26	13426.0
27	17610.0
28	23870.0
29	31918.0
30	41355.0
31	55293.0
32	76768.0
33	107495.0
34	226959.0
35	497157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94180018808095	17.351181394618457	11.694566530513189	26.012451886787403
2	16.48356647130231	20.002533106542998	37.29892351891312	26.21497690324157
3	18.818486522548042	24.054009700269173	28.710513500641273	28.416990276541508
4	12.577629641389631	15.564458802218004	36.03683232710988	35.82107922928249
5	14.483119097972649	36.71760089696098	33.55504634209214	15.244233662974224
6	33.55573712585319	35.877061843715545	17.05563543855768	13.511565591873579
7	29.670949221307747	30.63626356176995	21.169653036355623	18.52313418056668
8	27.964594032269	32.61945633049852	19.840778424521872	19.575171212710604
9	27.454294150347238	14.125291447569776	18.86875958665083	39.55165481543215
10	15.940519663172022	26.52698800255584	31.537643275041678	25.99484905923046
11	36.912991837546926	21.189092813882326	22.87628533872797	19.021630009842774
12	24.66425942518929	23.889343047974666	28.859499441324825	22.586898085511216
13	29.15751876880897	19.50742363067125	25.615809709444914	25.719247891074865
14	23.58889321247907	19.842779981222133	25.292377827836226	31.27594897846257
15	24.9685640672787	27.28647884533521	22.558728747836966	25.186228339549128
16	25.580002659600577	25.67219619641418	24.09776620325953	24.650034940725714
17	23.76356733736598	25.755585343259657	25.479910256112774	25.000937063261585
18	24.520004070258373	24.921317264143703	26.550402473574565	24.008276192023363
19	25.449542201538435	24.76396022000032	25.62948404176507	24.157013536696176
20	25.693967140885725	24.327723516421525	25.017986285791814	24.960323056900933
21	26.865646329849003	24.14212000010711	24.5035252407955	24.488708429248383
22	25.950660789805823	24.221699755249812	25.19454158878755	24.633097866156813
23	24.282471442126845	24.20838209394351	25.7156986402373	25.793447823692343
24	24.46763216031062	25.006582911208802	25.64041684332671	24.88536808515386
25	24.713165895170548	24.48002113655392	25.649672329858774	25.15714063841676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	273.0
1	273.0
2	383.0
3	493.0
4	493.0
5	493.0
6	1390.5
7	2288.0
8	2288.0
9	2288.0
10	2345.5
11	2403.0
12	2403.0
13	2403.0
14	2800.5
15	3198.0
16	3198.0
17	3198.0
18	5320.0
19	7442.0
20	7442.0
21	7442.0
22	12001.0
23	16560.0
24	16560.0
25	16560.0
26	24965.0
27	33370.0
28	33370.0
29	33370.0
30	42133.0
31	50896.0
32	50896.0
33	50896.0
34	61622.0
35	72348.0
36	72348.0
37	72348.0
38	82708.5
39	93069.0
40	93069.0
41	93069.0
42	105680.0
43	118291.0
44	118291.0
45	118291.0
46	131541.0
47	144791.0
48	144791.0
49	144791.0
50	148103.5
51	151416.0
52	151416.0
53	151416.0
54	139852.5
55	128289.0
56	128289.0
57	128289.0
58	117560.5
59	106832.0
60	106832.0
61	106832.0
62	94264.5
63	81697.0
64	81697.0
65	81697.0
66	66723.5
67	51750.0
68	51750.0
69	51750.0
70	38898.0
71	26046.0
72	26046.0
73	26046.0
74	20181.5
75	14317.0
76	14317.0
77	14317.0
78	11520.5
79	8724.0
80	8724.0
81	8724.0
82	6019.5
83	3315.0
84	3315.0
85	3315.0
86	2547.5
87	1780.0
88	1780.0
89	1780.0
90	1263.5
91	747.0
92	747.0
93	747.0
94	514.0
95	281.0
96	281.0
97	281.0
98	441.0
99	601.0
100	601.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03772686286419132
2	0.005708083270232257
3	9.81076812071169E-4
4	0.0024972864307266124
5	0.008740502507543144
6	0.011862110545951408
7	0.020959368257884067
8	0.034694443626880435
9	0.04619979896844233
10	0.05752677670780946
11	0.053334903056232646
12	0.06323485997804172
13	0.06608890161315785
14	0.06680241202193687
15	0.061005139950607246
16	0.0667132232208395
17	0.06198621676267841
18	0.07982397698215421
19	0.07625642493825906
20	0.08196450820849131
21	0.07741587935252497
22	0.0794672217777647
23	0.08455098344031531
24	0.07955641057886208
25	0.07875371136898567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1121217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.98039921117321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2472259562272	37.6088639282559
2	14.094874705666207	14.089349292413292
3	4.647150087831379	6.96799249752153
4	2.020155642089032	4.038727418412551
5	1.0445435774204175	2.610335249646974
6	0.6366504436882153	1.9092026000104532
7	0.4122706036484336	1.442381454736605
8	0.2967186826454417	1.1864094569626071
9	0.2208024124587624	0.9932213449331163
>10	1.1735309411801267	10.503795275492784
>50	0.0958011290470194	3.294197509960555
>100	0.0981025145771228	10.184524450851553
>500	0.008950958471037664	3.0564487676812093
>1k	0.003222345049573559	2.114550753120902
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3105	0.2769312274073618	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2118	0.18890188072424874	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1613	0.14386153617007236	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1381	0.12316973431548042	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1207	0.10765088292453645	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1196	0.10666980611246529	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1158	0.10328063167076489	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1145	0.10212117725649895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1142	0.10185361085320684	No Hit
GAACTACGACGGTATCTGATCGTCT	1142	0.10185361085320684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	1.7837760219475802E-4	0.0	0.0
6	0.0	0.0	1.7837760219475802E-4	0.0	0.0
7	0.0	0.0	1.7837760219475802E-4	0.0	0.0
8	0.0	0.0	1.7837760219475802E-4	0.0	0.0
9	0.0	0.0	1.7837760219475802E-4	0.0	0.0
10	0.0	0.0	1.7837760219475802E-4	0.0	0.0
11	0.0	0.0	1.7837760219475802E-4	0.0	0.0
12	0.0	0.0	1.7837760219475802E-4	0.0	5.351328065842741E-4
13	0.0	0.0	1.7837760219475802E-4	0.0	5.351328065842741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGC	60	1.4722864E-6	15.826752	2
AAAGACG	40	0.0052815066	14.248528	5
GCGTTAT	105	1.375156E-9	13.567604	1
TCCTAAA	120	5.2750693E-11	13.452739	2
CCGTGCG	50	0.0014977386	13.301593	8
TGCGTAC	50	0.0014982426	13.301	11
CGTTATT	100	1.0119038E-8	13.294471	2
GTATAAT	115	3.783498E-10	13.213667	1
GTCCTAA	130	1.4551915E-11	13.150139	1
CACGCCC	95	7.3678166E-8	13.001557	16
CGTCTTA	60	4.0863067E-4	12.668185	15
CCGTTAA	60	4.0863067E-4	12.668185	16
TTAGAAC	60	4.1017233E-4	12.662531	3
TTTATAC	60	4.1017233E-4	12.662531	3
CGACCAT	160	0.0	12.470244	10
GCGCCCC	100	1.438766E-7	12.35148	15
ACGGTAT	270	0.0	11.963862	9
TCCAACG	80	2.859868E-5	11.876953	18
CGGTGAG	105	2.7128226E-7	11.763315	15
GGTCGCG	130	2.6066118E-9	11.693709	7
>>END_MODULE