FastQCFastQC Report
Thu 2 Feb 2017
SRR4064234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121217
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13440.1198697486748774No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12020.10720493891904956No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11760.10488603009051771No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11710.10444008608503082No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11500.10256712126198587No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11260.10042659003564876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.022.9219931
AACGCAG29350.019.4887896
GTATAGT808.988319E-519.2498651
GTACTAT1152.0212246E-719.13031
GTCGTAA1002.4810006E-517.60144630
GGTCGTA1002.4819517E-517.6006629
ACCGTCG4400.017.4990948
TACCGTC4600.017.217277
TAATACT2055.456968E-1217.170614
TCAACGC33250.017.136724
CAACGCA33600.016.9582145
ATCAACG33750.016.9480273
CCGTCGT4950.016.888779
TATCAAC34500.016.8339122
TAGCGGC3550.016.73376830
GTGTATA3550.016.7322751
ACTAGGG800.001988323616.4998843
CGCAGAG34700.016.4832978
ACGCAGA34800.016.4366657
CGTCGTA4850.016.32905210
Produced by FastQC (version 0.11.5)