##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1121217
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300922123014544	32.0	32.0	32.0	32.0	32.0
2	31.374833774371954	32.0	32.0	32.0	32.0	32.0
3	31.445484683161244	32.0	32.0	32.0	32.0	32.0
4	31.533615705077608	32.0	32.0	32.0	32.0	32.0
5	31.483605760526284	32.0	32.0	32.0	32.0	32.0
6	35.041457630414094	36.0	36.0	36.0	36.0	36.0
7	35.066297603407726	36.0	36.0	36.0	36.0	36.0
8	35.02117609704455	36.0	36.0	36.0	36.0	36.0
9	35.11576082060832	36.0	36.0	36.0	36.0	36.0
10	34.97666553396889	36.0	36.0	36.0	36.0	36.0
11	35.11744559706105	36.0	36.0	36.0	36.0	36.0
12	35.021115448659806	36.0	36.0	36.0	36.0	36.0
13	35.0676443543043	36.0	36.0	36.0	36.0	36.0
14	35.0059248120569	36.0	36.0	36.0	36.0	36.0
15	34.98288912850947	36.0	36.0	36.0	36.0	36.0
16	34.99410105269542	36.0	36.0	36.0	36.0	36.0
17	34.954401333550955	36.0	36.0	36.0	36.0	36.0
18	34.952315207493285	36.0	36.0	36.0	36.0	36.0
19	34.949593165283794	36.0	36.0	36.0	36.0	36.0
20	34.939206237507996	36.0	36.0	36.0	36.0	36.0
21	34.94077596040731	36.0	36.0	36.0	36.0	36.0
22	34.92360354864402	36.0	36.0	36.0	32.0	36.0
23	34.870818048602544	36.0	36.0	36.0	32.0	36.0
24	34.837002114666475	36.0	36.0	36.0	32.0	36.0
25	34.81396643111904	36.0	36.0	36.0	32.0	36.0
26	34.75526057846073	36.0	36.0	36.0	32.0	36.0
27	34.74831901407132	36.0	36.0	36.0	32.0	36.0
28	34.72929504279725	36.0	36.0	36.0	32.0	36.0
29	34.6946032748344	36.0	36.0	36.0	32.0	36.0
30	34.675724681306114	36.0	36.0	36.0	32.0	36.0
31	34.66708674591983	36.0	36.0	36.0	32.0	36.0
32	34.621107243290105	36.0	36.0	36.0	32.0	36.0
33	34.596883564912055	36.0	36.0	36.0	32.0	36.0
34	34.58824027819771	36.0	36.0	36.0	32.0	36.0
35	34.54956177082581	36.0	36.0	36.0	32.0	36.0
36	34.516699265173465	36.0	36.0	36.0	32.0	36.0
37	34.50860627336189	36.0	36.0	36.0	32.0	36.0
38	34.471294138422806	36.0	36.0	36.0	32.0	36.0
39	34.46512673282692	36.0	36.0	36.0	32.0	36.0
40	34.438944468376775	36.0	36.0	36.0	32.0	36.0
41	34.41542716530341	36.0	36.0	36.0	32.0	36.0
42	34.35954057064779	36.0	36.0	36.0	32.0	36.0
43	34.34989658558513	36.0	36.0	36.0	32.0	36.0
44	34.27886305683913	36.0	36.0	36.0	32.0	36.0
45	34.24159284063656	36.0	36.0	36.0	32.0	36.0
46	34.20782596054109	36.0	36.0	36.0	32.0	36.0
47	34.190610738153275	36.0	36.0	36.0	32.0	36.0
48	34.13098356517962	36.0	36.0	36.0	32.0	36.0
49	34.10976644128657	36.0	36.0	36.0	32.0	36.0
50	33.507099874511354	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	30.0
22	110.0
23	361.0
24	988.0
25	2236.0
26	4467.0
27	8235.0
28	13783.0
29	21294.0
30	30424.0
31	42663.0
32	61246.0
33	96778.0
34	198029.0
35	640562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.021479164381034	18.43768758925123	12.201942142457675	26.33889110391006
2	15.82928489056049	20.940534938499642	36.902087292958605	26.328092877981256
3	17.99374248581	24.767037812652738	29.07335989982269	28.16585980171457
4	12.24383861464819	16.261348160079628	36.24320715793642	35.251606067335764
5	14.158008663800139	37.112976346238064	33.38533040437311	15.343684585588695
6	33.75912180903914	36.231263612477186	16.657836951732673	13.351777626751005
7	29.557132218174036	30.957309704204818	21.11566470302306	18.369893374598092
8	27.90824612898306	32.97131598967907	19.56775539436166	19.552682486976206
9	27.62048654838905	13.937517169043279	18.66671899136999	39.775277291197675
10	15.888769870327247	26.80352512961478	31.621190377869564	25.68651462218841
11	37.28193318682573	21.137675523717107	22.688670158114046	18.891721131343115
12	24.700793335800896	23.84279553876821	28.880277199288273	22.57613392614262
13	29.28678965262591	19.598345361983284	25.525146002718486	25.58971898267232
14	23.660629476720384	19.84441905536573	25.25782252677225	31.237128941141634
15	25.14317568622204	27.37181974268067	22.4741900238589	25.010814547238386
16	25.581449277430483	25.786139401555648	24.03998547993368	24.592425841080193
17	23.894036569192227	25.75353388327148	25.429867724089096	24.9225618234472
18	24.591915548682053	24.87946101279506	26.59724191813516	23.931381520387724
19	25.54902396235519	24.706724924791544	25.691280100105512	24.052971012747758
20	25.945920876482443	24.164718620703187	25.045642694626963	24.843717808187414
21	27.128825196193063	24.03415217571621	24.54556076120858	24.29146186688215
22	25.99492514816516	24.23968641161597	25.27213781478129	24.493250625437582
23	24.430322733130872	24.153031782769432	25.776552905133315	25.640092578966378
24	24.786615222428544	24.931814979120844	25.56309500238135	24.718474796069263
25	24.827997709609118	24.267351405540104	25.840300533532645	25.064350351318133
26	24.412326886730607	25.313001991688957	26.054020883746194	24.220650237834242
27	25.12556225399297	24.776547798390414	25.255517291512252	24.842372656104363
28	24.435992985923683	24.77795596401629	25.99079179502914	24.79525925503089
29	24.572843390923772	24.563121603506268	25.772726015949083	25.091308989620874
30	24.416572940757796	25.11922738336587	25.94773171408261	24.516467961793722
31	25.031686525934788	24.784437968886998	25.061923633019635	25.121951872158583
32	24.764423355646034	25.070884822754497	24.873861137992392	25.290830683607073
33	24.451445049069147	24.49782504573335	25.518987703057423	25.53174220214008
34	25.169754944589386	24.700604276763215	25.634624389591277	24.495016389056122
35	25.69390682026565	24.43229859755902	25.568593313521013	24.30520126865432
36	24.35657870678006	25.304886478676007	25.280893364432217	25.057641450111717
37	25.504891716859273	25.07766364217731	24.978036552528092	24.439408088435318
38	24.685743133979166	24.814005781630613	25.425081858862924	25.0751692255273
39	25.429036501884184	24.738867705755123	24.74190023858898	25.09019555377171
40	25.545422517938448	24.971213749615366	25.312635179429094	24.170728553017092
41	24.43831398760946	25.33317635558315	25.803574454725297	24.424935202082096
42	25.88619020267498	25.53085789918694	24.94505906150888	23.637892836629195
43	24.985618100590347	24.55741655599665	25.49114743921919	24.965817904193806
44	24.59260422027333	25.184644762296347	24.87372982865787	25.349021188772458
45	24.75115858422613	25.49839635532057	24.954869621368612	24.79557543908469
46	24.704359839494973	25.07440615548724	24.875514064703257	25.345719940314538
47	24.86625817212069	24.98898491781054	25.354578617361913	24.790178292706855
48	25.56038173385658	25.4709240099893	24.372814841241528	24.595879414912595
49	24.763336883057114	25.565413917414514	24.73167477395038	24.93957442557799
50	24.892571035117545	25.776529915109265	24.50646892720874	24.824430122564454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	82.0
2	38.0
3	111.5
4	185.0
5	215.5
6	246.0
7	211.5
8	177.0
9	283.5
10	390.0
11	591.0
12	792.0
13	1399.0
14	2006.0
15	2648.0
16	3290.0
17	3530.5
18	3771.0
19	3710.5
20	3650.0
21	4010.5
22	4371.0
23	4964.5
24	5558.0
25	6383.0
26	7208.0
27	9126.0
28	11044.0
29	12626.0
30	14208.0
31	16058.0
32	17908.0
33	20850.0
34	23792.0
35	28062.0
36	32332.0
37	37794.0
38	43256.0
39	46651.5
40	50047.0
41	54100.0
42	58153.0
43	59372.5
44	60592.0
45	66726.0
46	72860.0
47	77506.0
48	82152.0
49	84588.0
50	87024.0
51	84192.5
52	81361.0
53	80472.0
54	79583.0
55	79677.0
56	79771.0
57	77900.0
58	76029.0
59	69447.5
60	62866.0
61	54687.5
62	46509.0
63	40359.5
64	34210.0
65	28806.5
66	23403.0
67	20361.0
68	17319.0
69	15618.0
70	13917.0
71	10705.0
72	7493.0
73	6411.0
74	5329.0
75	4036.5
76	2744.0
77	2459.0
78	2174.0
79	1788.5
80	1403.0
81	1156.5
82	910.0
83	744.0
84	578.0
85	392.0
86	206.0
87	153.0
88	100.0
89	69.5
90	39.0
91	38.0
92	37.0
93	25.5
94	14.0
95	20.0
96	26.0
97	16.5
98	7.0
99	10.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007491859292179838
2	3.5675520438951605E-4
3	4.4594400548689505E-4
4	0.0
5	0.0
6	2.6756640329213705E-4
7	1.7837760219475802E-4
8	0.0
9	0.0011594544142659272
10	8.918880109737901E-4
11	8.918880109737901E-5
12	1.7837760219475802E-4
13	4.4594400548689505E-4
14	0.0
15	0.003745929646089919
16	8.918880109737901E-4
17	0.0
18	2.6756640329213705E-4
19	0.0
20	7.135104087790321E-4
21	0.0
22	1.7837760219475802E-4
23	0.0010702656131685482
24	2.6756640329213705E-4
25	9.81076812071169E-4
26	0.005172950463647983
27	0.005351328065842741
28	0.003835118447187297
29	0.0021405312263370964
30	0.0035675520438951604
31	0.006956726485595563
32	0.0028540416351161284
33	0.003924307248284676
34	0.003745929646089919
35	0.0025864752318239914
36	0.005529705668037498
37	0.003210796839505644
38	0.007135104087790321
39	0.003745929646089919
40	0.001962153624142338
41	0.0034783632427977814
42	0.0011594544142659272
43	0.0014270208175580642
44	8.918880109737901E-4
45	0.0018729648230449594
46	5.351328065842741E-4
47	0.002408097629629233
48	0.0015162096186554432
49	2.6756640329213705E-4
50	9.81076812071169E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1121217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.14315091338552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10150253318244	40.442736338563044
2	13.862780227715335	14.73423643441146
3	4.472439940672541	7.1303865215464155
4	1.8874490640916557	4.012199618174044
5	0.9953661493648767	2.644844674488654
6	0.5995292262992737	1.9116523290124539
7	0.39242718298267354	1.459837190543408
8	0.27114592953870714	1.1527639242420569
9	0.20057606898541985	0.9593319873335213
>10	1.0451179309909968	9.893221951483934
>50	0.08028591348423798	2.9301385762060845
>100	0.0816011809425306	8.778991679087754
>500	0.007924079865884654	2.838822844339226
>1k	0.001854571883504919	1.1108359305680384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1344	0.1198697486748774	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1202	0.10720493891904956	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1176	0.10488603009051771	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1171	0.10444008608503082	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1150	0.10256712126198587	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1126	0.10042659003564876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	8.918880109737901E-5	0.0	0.0
11	0.0	0.0	8.918880109737901E-5	0.0	0.0
12	0.0	0.0	8.918880109737901E-5	0.0	0.0
13	0.0	0.0	8.918880109737901E-5	0.0	8.918880109737901E-5
14	0.0	0.0	8.918880109737901E-5	0.0	8.918880109737901E-5
15	0.0	0.0	8.918880109737901E-5	0.0	1.7837760219475802E-4
16	0.0	0.0	8.918880109737901E-5	0.0	1.7837760219475802E-4
17	0.0	0.0	8.918880109737901E-5	0.0	1.7837760219475802E-4
18	0.0	0.0	8.918880109737901E-5	0.0	2.6756640329213705E-4
19	0.0	0.0	8.918880109737901E-5	0.0	2.6756640329213705E-4
20	0.0	0.0	8.918880109737901E-5	0.0	2.6756640329213705E-4
21	0.0	0.0	8.918880109737901E-5	0.0	2.6756640329213705E-4
22	0.0	0.0	8.918880109737901E-5	2.6756640329213705E-4	2.6756640329213705E-4
23	0.0	0.0	8.918880109737901E-5	5.351328065842741E-4	2.6756640329213705E-4
24	0.0	0.0	8.918880109737901E-5	0.0013378320164606852	2.6756640329213705E-4
25	0.0	0.0	8.918880109737901E-5	0.0015162096186554432	2.6756640329213705E-4
26	0.0	0.0	8.918880109737901E-5	0.0015162096186554432	2.6756640329213705E-4
27	0.0	0.0	8.918880109737901E-5	0.0023189088285318544	4.4594400548689505E-4
28	0.0	0.0	8.918880109737901E-5	0.004459440054868951	4.4594400548689505E-4
29	0.0	0.0	8.918880109737901E-5	0.008294558502056248	4.4594400548689505E-4
30	0.0	0.0	8.918880109737901E-5	0.014894529783262294	4.4594400548689505E-4
31	0.0	0.0	8.918880109737901E-5	0.027380961936895355	4.4594400548689505E-4
32	0.0	0.0	8.918880109737901E-5	0.04307819093003406	4.4594400548689505E-4
33	0.0	0.0	8.918880109737901E-5	0.05824028711658849	4.4594400548689505E-4
34	0.0	0.0	8.918880109737901E-5	0.0717969848833901	4.4594400548689505E-4
35	0.0	0.0	8.918880109737901E-5	0.09409418515773485	4.4594400548689505E-4
36	0.0	0.0	8.918880109737901E-5	0.1245075663319411	4.4594400548689505E-4
37	0.0	0.0	8.918880109737901E-5	0.17222357491903886	4.4594400548689505E-4
38	0.0	0.0	8.918880109737901E-5	0.2311773724444064	4.4594400548689505E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2505	0.0	22.921993	1
AACGCAG	2935	0.0	19.488789	6
GTATAGT	80	8.988319E-5	19.249865	1
GTACTAT	115	2.0212246E-7	19.1303	1
GTCGTAA	100	2.4810006E-5	17.601446	30
GGTCGTA	100	2.4819517E-5	17.60066	29
ACCGTCG	440	0.0	17.499094	8
TACCGTC	460	0.0	17.21727	7
TAATACT	205	5.456968E-12	17.17061	4
TCAACGC	3325	0.0	17.13672	4
CAACGCA	3360	0.0	16.958214	5
ATCAACG	3375	0.0	16.948027	3
CCGTCGT	495	0.0	16.88877	9
TATCAAC	3450	0.0	16.833912	2
TAGCGGC	355	0.0	16.733768	30
GTGTATA	355	0.0	16.732275	1
ACTAGGG	80	0.0019883236	16.499884	3
CGCAGAG	3470	0.0	16.483297	8
ACGCAGA	3480	0.0	16.436665	7
CGTCGTA	485	0.0	16.329052	10
>>END_MODULE