FastQCFastQC Report
Thu 2 Feb 2017
SRR4064233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646765
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42200.65247810255657No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34220.5290948025944509No Hit
TATCAACGCAGAGTACTTTTTTTTT33280.5145609301678353No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23490.3631921950012756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18470.28557513161658404No Hit
GTACATGGGGTGGTATCAACGCAAA18290.28279204966255134No Hit
GGTATCAACGCAGAGTACTTTTTTT14770.2283673358947995No Hit
GTATCAACGCAGAGTACATGGGGTG9950.15384258579236662No Hit
GTACATGGGTGGTATCAACGCAAAA9690.1498225785254304No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8730.13497947477058903No Hit
TATCAACGCAGAGTACATGGGGTGG7630.11797175171816655No Hit
GTACATGGAAGCAGTGGTATCAACG7560.11688944206937604No Hit
GTACATGGGAGTGGTATCAACGCAA7440.11503405410002088No Hit
TTGTAGAACAGTGTATATCAATGAG7080.10946789019195535No Hit
GATATACACTGTTCTACAAATCCCG6900.10668480823792259No Hit
TCCATGTACTCTGCGTTGATACCAC6550.10127325999396998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG250.006024665719.0020127
CTATAGC402.7606438E-416.6229024
TACCGTC551.1251457E-515.54717
ATACCGT551.1259712E-515.5458986
CGGTCGG400.005270984514.25150910
GTCGGCG400.005270984514.25150912
TCCAACG801.2839882E-714.25040618
CCGTCGT551.9556083E-413.8196459
ACCGTCG551.9556083E-413.8196458
AGAACCG551.9581773E-413.8175065
CGTCGTA655.4374766E-513.15523910
CCCTTAA655.449333E-513.1521851
TCGTTAA801.9918825E-613.06388312
TTACACA801.997083E-613.0608524
TTAGGAC1403.6379788E-1212.8912313
ATCCTCG905.3816984E-712.6680098
GACGTGA1051.9823347E-812.6680087
CGACCCG604.087967E-412.6660485
ATTTAGA604.090636E-412.6650681
CTCTTAA1207.385097E-1012.6650681
Produced by FastQC (version 0.11.5)