##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064233_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646765
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.107903179671133	32.0	32.0	32.0	32.0	32.0
2	30.836068742124265	32.0	32.0	32.0	32.0	32.0
3	30.86748046044545	32.0	32.0	32.0	32.0	32.0
4	30.850846907300177	32.0	32.0	32.0	32.0	32.0
5	30.8682380771996	32.0	32.0	32.0	32.0	32.0
6	34.37549496339474	36.0	36.0	36.0	32.0	36.0
7	34.27418304948474	36.0	36.0	36.0	32.0	36.0
8	34.23250948953638	36.0	36.0	36.0	32.0	36.0
9	34.31840776789097	36.0	36.0	36.0	32.0	36.0
10	34.088319559654586	36.0	36.0	36.0	32.0	36.0
11	34.368241942591204	36.0	36.0	36.0	32.0	36.0
12	34.20457816981438	36.0	36.0	36.0	32.0	36.0
13	34.280986138705714	36.0	36.0	36.0	32.0	36.0
14	34.177843575332616	36.0	36.0	36.0	32.0	36.0
15	34.12700594497228	36.0	36.0	36.0	32.0	36.0
16	34.14996482493641	36.0	36.0	36.0	32.0	36.0
17	34.08987808554885	36.0	36.0	36.0	32.0	36.0
18	34.07970282869358	36.0	36.0	36.0	32.0	36.0
19	34.05321716543103	36.0	36.0	36.0	32.0	36.0
20	33.98429568699605	36.0	36.0	36.0	32.0	36.0
21	33.95292107643425	36.0	36.0	36.0	32.0	36.0
22	33.92625915131462	36.0	36.0	36.0	32.0	36.0
23	33.89731046052275	36.0	36.0	36.0	32.0	36.0
24	33.855248815257475	36.0	36.0	36.0	32.0	36.0
25	33.24749174738893	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	8.0
6	22.0
7	8.0
8	36.0
9	36.0
10	67.0
11	17.0
12	38.0
13	39.0
14	174.0
15	309.0
16	472.0
17	599.0
18	835.0
19	936.0
20	1223.0
21	1494.0
22	1865.0
23	2593.0
24	3378.0
25	4456.0
26	6390.0
27	8435.0
28	11455.0
29	15876.0
30	22442.0
31	32226.0
32	49190.0
33	74045.0
34	161243.0
35	246853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.769008873565994	17.69779143021096	11.841423482700646	24.691776213522402
2	15.631122154720137	20.566728877164934	38.99021073741861	24.81193823069632
3	18.960444185374108	25.633274939428563	28.90482824306502	26.501452632132317
4	11.953533535576563	15.744666375980678	36.41800645075501	35.88379363768775
5	13.357919314685098	37.95874503254936	33.85257688917753	14.830758763588006
6	32.55051926443209	36.1853702890438	17.679268390744166	13.584842055779948
7	28.559719657675547	31.272520328220736	21.552862652674293	18.614897361429424
8	27.87159340489374	33.19423392210845	19.99412256008909	18.940050112908715
9	27.440425087593106	14.145454826708741	19.42238825594976	38.9917318297484
10	15.649776685916903	27.60044492643089	32.30667126650877	24.44310712114344
11	36.21330866359533	21.69070212838371	23.33579824444699	18.76019096357398
12	24.39794172341986	24.36792776847792	30.16448884997045	21.069641658131772
13	29.52140044711579	20.10721496368151	26.416807840771085	23.954576748431613
14	23.22002404296171	20.280408389972507	26.85122295746995	29.648344609595835
15	24.28717739265503	27.90010117981231	24.41759724246334	23.395124185069324
16	23.967150568660912	26.325341188811514	26.138443519926724	23.56906472260085
17	22.278469262247526	26.207734139907636	27.04365248199519	24.47014411584965
18	22.835480252214616	25.8533905845035	28.86310007349812	22.44802908978376
19	24.449983676286053	25.132175664823862	27.37494565208982	23.042895006800258
20	24.599234987559264	25.140654593169355	27.151442754044787	23.108667665226594
21	25.824170723178284	24.538671733566872	26.269174947314344	23.367982595940497
22	24.3516365172425	25.220608373138	26.759412701286283	23.668342408333217
23	23.609331575461596	25.04858906034328	27.296529441256755	24.04554992293837
24	23.518780704808325	26.273799853003755	27.062009206607097	23.14541023558083
25	23.92354862618701	25.47832397445638	27.314561938022518	23.283565461334092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	229.0
1	229.0
2	514.0
3	799.0
4	799.0
5	799.0
6	2469.0
7	4139.0
8	4139.0
9	4139.0
10	3915.0
11	3691.0
12	3691.0
13	3691.0
14	2983.0
15	2275.0
16	2275.0
17	2275.0
18	4115.0
19	5955.0
20	5955.0
21	5955.0
22	9412.5
23	12870.0
24	12870.0
25	12870.0
26	19517.0
27	26164.0
28	26164.0
29	26164.0
30	32558.5
31	38953.0
32	38953.0
33	38953.0
34	42570.0
35	46187.0
36	46187.0
37	46187.0
38	49944.0
39	53701.0
40	53701.0
41	53701.0
42	60332.0
43	66963.0
44	66963.0
45	66963.0
46	76538.0
47	86113.0
48	86113.0
49	86113.0
50	84806.5
51	83500.0
52	83500.0
53	83500.0
54	75133.5
55	66767.0
56	66767.0
57	66767.0
58	60534.0
59	54301.0
60	54301.0
61	54301.0
62	47658.5
63	41016.0
64	41016.0
65	41016.0
66	33446.0
67	25876.0
68	25876.0
69	25876.0
70	19567.5
71	13259.0
72	13259.0
73	13259.0
74	10088.5
75	6918.0
76	6918.0
77	6918.0
78	5502.5
79	4087.0
80	4087.0
81	4087.0
82	2783.0
83	1479.0
84	1479.0
85	1479.0
86	1134.0
87	789.0
88	789.0
89	789.0
90	539.0
91	289.0
92	289.0
93	289.0
94	183.5
95	78.0
96	78.0
97	78.0
98	222.5
99	367.0
100	367.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03679852805887764
2	0.005566163908065526
3	0.0012369253129034502
4	0.0029376976181456943
5	0.00850386152621122
6	0.012523868793147434
7	0.019636189342342274
8	0.03478852442540954
9	0.0479308558750087
10	0.056589333065332856
11	0.05550702341654233
12	0.06169164998105958
13	0.06184626564517251
14	0.065247810255657
15	0.060454724668156136
16	0.065247810255657
17	0.06339242228630182
18	0.07576167541533632
19	0.07205089947662598
20	0.07746244772057857
21	0.07406090311009408
22	0.07483398143065874
23	0.08179168631574064
24	0.07576167541533632
25	0.07699860072823977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	646765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.031376400473604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98174045105749	40.82447653823595
2	13.78812991236825	14.624070144828597
3	4.15785082410817	6.614896562108992
4	1.7483197072736156	3.7086320185917176
5	0.8850397459087407	2.346743794733297
6	0.508899914449219	1.619259774799521
7	0.3296729865129276	1.2238108565784727
8	0.22648933768266186	0.9608833053874568
9	0.16997688875566377	0.8112697530284733
>10	0.9900993424414938	9.830497142025024
>50	0.12482705504559385	4.682081197817369
>100	0.083995751823297	8.704660188459505
>500	0.0029165191605302772	1.1917094305627196
>1k	0.002041563412371194	2.8570092928429016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4220	0.65247810255657	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3422	0.5290948025944509	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3328	0.5145609301678353	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2349	0.3631921950012756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1847	0.28557513161658404	No Hit
GTACATGGGGTGGTATCAACGCAAA	1829	0.28279204966255134	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1477	0.2283673358947995	No Hit
GTATCAACGCAGAGTACATGGGGTG	995	0.15384258579236662	No Hit
GTACATGGGTGGTATCAACGCAAAA	969	0.1498225785254304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	873	0.13497947477058903	No Hit
TATCAACGCAGAGTACATGGGGTGG	763	0.11797175171816655	No Hit
GTACATGGAAGCAGTGGTATCAACG	756	0.11688944206937604	No Hit
GTACATGGGAGTGGTATCAACGCAA	744	0.11503405410002088	No Hit
TTGTAGAACAGTGTATATCAATGAG	708	0.10946789019195535	No Hit
GATATACACTGTTCTACAAATCCCG	690	0.10668480823792259	No Hit
TCCATGTACTCTGCGTTGATACCAC	655	0.10127325999396998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.5461566411293127E-4
13	0.0	0.0	0.0	0.0	3.0923132822586254E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	25	0.0060246657	19.002012	7
CTATAGC	40	2.7606438E-4	16.622902	4
TACCGTC	55	1.1251457E-5	15.5471	7
ATACCGT	55	1.1259712E-5	15.545898	6
CGGTCGG	40	0.0052709845	14.251509	10
GTCGGCG	40	0.0052709845	14.251509	12
TCCAACG	80	1.2839882E-7	14.250406	18
CCGTCGT	55	1.9556083E-4	13.819645	9
ACCGTCG	55	1.9556083E-4	13.819645	8
AGAACCG	55	1.9581773E-4	13.817506	5
CGTCGTA	65	5.4374766E-5	13.155239	10
CCCTTAA	65	5.449333E-5	13.152185	1
TCGTTAA	80	1.9918825E-6	13.063883	12
TTACACA	80	1.997083E-6	13.060852	4
TTAGGAC	140	3.6379788E-12	12.891231	3
ATCCTCG	90	5.3816984E-7	12.668009	8
GACGTGA	105	1.9823347E-8	12.668008	7
CGACCCG	60	4.087967E-4	12.666048	5
ATTTAGA	60	4.090636E-4	12.665068	1
CTCTTAA	120	7.385097E-10	12.665068	1
>>END_MODULE