FastQCFastQC Report
Thu 2 Feb 2017
SRR4064233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646765
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14210.21970885870447535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.1666756859137399No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA8040.12431099394679675No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7370.11395174445123035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA1107.2759576E-1225.9987244
TTCGGGC1306.730261E-1122.0006235
GTAAACG1306.730261E-1122.0006227
CTAGACC500.002579889721.9989173
TTGCCGA602.8717343E-421.99891741
AGTAAAC1451.2732926E-1121.24033526
TAAACGC1351.1277734E-1021.1857828
TAACGGC1259.931682E-1021.12059436
CGCTTCG1502.1827873E-1120.53549832
AAACGCT1452.9467628E-1019.72469329
CGCAATA1152.0171865E-719.13097236
GTATATC3800.019.1043223
ACGCTTC1504.638423E-1019.06867631
ACTATAC708.1204314E-418.8562163
TAGACAA708.1204314E-418.8562165
TTATGCC600.007410574218.3324323
TTAACGG1456.2882464E-918.20740935
ATTTCGT1456.2937033E-918.20600142
AACGCTT1701.2551027E-1018.11955830
GCATATA851.4300687E-418.1167562
Produced by FastQC (version 0.11.5)