FastQCFastQC Report
Thu 2 Feb 2017
SRR4064232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759078
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT59390.7823965389591057No Hit
GTCCTACAGTGGACATTTCTAAATT54160.7134971636643401No Hit
CTGTAGGACGTGGAATATGGCAAGA50470.6648855585328517No Hit
CTTTAGGACGTGAAATATGGCGAGG47530.6261543609484137No Hit
GTATCAACGCAGAGTACTTTTTTTT40930.5392067745343694No Hit
GTCCTACAGTGTGCATTTCTCATTT35950.47360086842195404No Hit
TATCAACGCAGAGTACTTTTTTTTT27940.36807811581945465No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25530.336329072901599No Hit
CTGTAGGACCTGGAATATGGCGAGA25380.33435299139218894No Hit
CTGAAGGACCTGGAATATGGCGAGA23290.30681958902774153No Hit
ATTTAGAAATGTCCACTGTAGGACG22500.29641222641151505No Hit
GTCCTTCAGTGTGCATTTCTCATTT20880.27507054610988596No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18320.24134542168262021No Hit
TTTCTAAATTTTCCACCTTTTTCAG16990.22382416563251734No Hit
GAATATGGCAAGAAAACTGAAAATC16630.21908157000993309No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15390.2027459628654763No Hit
GGTATCAACGCAGAGTACTTTTTTT14160.18654209448831346No Hit
GGAATATGGCGAGAAAACTGAAAAT13790.18166776009843522No Hit
CTGTAGGACATGGAATATGGCAAGA12400.1633560714445683No Hit
CCATATTCCAGGTCCTTCAGTGTGC11920.15703261061445598No Hit
GATATACACTGTTCTACAAATCCCG11660.1536074026648118No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11540.1520265374572837No Hit
TTGTAGAACAGTGTATATCAATGAG11530.1518947986899897No Hit
TTCCAGGTCCTTCAGTGTGCATTTC11270.14846959074034552No Hit
GCCATATTCCACGTCCTACAGTGGA11100.1462300316963474No Hit
ACCTGGAATATGGCGAGAAAACTGA10990.14478090525611334No Hit
GTACATGGGGTGGTATCAACGCAAA10550.13898439949517705No Hit
GAAATATGGCGAGGAAAACTGAAAA10510.13845744442600103No Hit
CCATATTTCACGTCCTAAAGTGTGT10360.1364813629165909No Hit
GACCTGGAATATGGCGAGAAAACTG9970.13134355099212466No Hit
ACAGTGGACATTTCTAAATTTTCCA9580.1262057390676584No Hit
GTCCACTGTAGGACGTGGAATATGG9380.12357096372177827No Hit
GTGTATATCAATGAGTTACAATGAA9340.12304400865260225No Hit
ATACACACTTTAGGACGTGAAATAT8950.11790619672813597No Hit
GAAATACACACTTTAGGACGTGAAA8710.11474446631307982No Hit
CCACTGTAGGACGTGGAATATGGCA8410.11079230329425961No Hit
GTGTATTTCTCATTTTCCGTGATTT8350.11000187069049557No Hit
AGTGTGTATTTCTCATTTTCCGTGA7940.10460058123144131No Hit
CCTAAAGTGTGTATTTCTCATTTTC7900.10407362616226529No Hit
CACTTTAGGACGTGAAATATGGCGA7760.1022292834201492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTG359.937188E-518.9984846
TTTCGAA402.756297E-416.62696316
GTCCTAG1600.015.435251
TCGAAAT508.6958076E-515.20179518
AATGGCG653.3625056E-614.61710916
GTCTTAC851.715307E-814.5272941
TCCTAGA1700.014.5234662
CCAACGT602.5619898E-514.2516819
CGTGTGC400.005273984714.2507418
CAGTCCC400.005273984714.2507419
CCAAGGC400.00527860114.2488623
AGTAGTG400.00527860114.2488625
GATCTAG400.0052809114.2479241
GTGTAGC759.669802E-713.9313031
TTCGAAA551.9572055E-413.81890217
CCAACGA1450.013.76024419
TAGGACC7600.013.2489434
GACTGTT655.4426513E-513.15453157
TGGACTG655.4493903E-513.1527975
TCCAACG2100.013.12059518
Produced by FastQC (version 0.11.5)