##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064232_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	759078
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198360379302258	32.0	32.0	32.0	32.0	32.0
2	30.95583194348934	32.0	32.0	32.0	32.0	32.0
3	30.972786722840077	32.0	32.0	32.0	32.0	32.0
4	30.97328601276812	32.0	32.0	32.0	32.0	32.0
5	30.98574718276646	32.0	32.0	32.0	32.0	32.0
6	34.618649203375675	36.0	36.0	36.0	32.0	36.0
7	34.522959432364	36.0	36.0	36.0	32.0	36.0
8	34.469976734933695	36.0	36.0	36.0	32.0	36.0
9	34.52012441409183	36.0	36.0	36.0	32.0	36.0
10	34.33799029875718	36.0	36.0	36.0	32.0	36.0
11	34.558704112093885	36.0	36.0	36.0	32.0	36.0
12	34.42844608854426	36.0	36.0	36.0	32.0	36.0
13	34.457220470096615	36.0	36.0	36.0	32.0	36.0
14	34.417563148978104	36.0	36.0	36.0	32.0	36.0
15	34.39579331768277	36.0	36.0	36.0	32.0	36.0
16	34.39691441459244	36.0	36.0	36.0	32.0	36.0
17	34.373082871588956	36.0	36.0	36.0	32.0	36.0
18	34.38080935029075	36.0	36.0	36.0	32.0	36.0
19	34.307815534108485	36.0	36.0	36.0	32.0	36.0
20	34.279229538993356	36.0	36.0	36.0	32.0	36.0
21	34.25006125852679	36.0	36.0	36.0	32.0	36.0
22	34.22195084036159	36.0	36.0	36.0	32.0	36.0
23	34.24667293743199	36.0	36.0	36.0	32.0	36.0
24	34.210717475674436	36.0	36.0	36.0	32.0	36.0
25	33.89154737721288	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	11.0
5	7.0
6	40.0
7	5.0
8	56.0
9	55.0
10	85.0
11	20.0
12	52.0
13	39.0
14	105.0
15	126.0
16	247.0
17	298.0
18	294.0
19	455.0
20	602.0
21	807.0
22	1383.0
23	2161.0
24	3151.0
25	4737.0
26	7098.0
27	9679.0
28	13327.0
29	18713.0
30	24928.0
31	34817.0
32	50229.0
33	70721.0
34	157248.0
35	357580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.007343328272604	20.777849822874494	12.748160214237517	24.466646634615387
2	11.121841067245645	21.506301397294997	48.55619876738714	18.81565876807222
3	18.147218152883024	28.459919058208534	30.49993149457753	22.89293129433091
4	9.935142014352284	17.72429229964574	41.340740906641656	30.999824779360324
5	9.506807045651279	41.99314091170802	37.187562994652126	11.312489047988585
6	27.389993622818714	40.831089022288516	20.207232039802044	11.571685315090729
7	25.2711586787249	33.3725142415134	23.869079542953827	17.48724753680787
8	22.131986920713747	43.05726339262768	21.05878430116625	13.751965385492326
9	27.42475269058148	14.728239736906277	22.071138117614495	35.775869454897745
10	14.503807737279969	30.3172872722575	36.572074132564886	18.606830857897645
11	32.39266181303363	21.737737721903436	29.377002848642313	16.492597616420625
12	25.1574366660998	24.850538982465437	35.080989085885214	14.911035265549549
13	29.76193928911365	24.63594824835936	27.135335233459802	18.466777229067187
14	18.191765796242414	26.01701473529338	29.409678597371986	26.38154087109222
15	18.865447182337338	39.7934540162305	24.177665402888586	17.163433398543578
16	16.769781830022794	29.24524446401428	35.67459464732769	18.31037905863524
17	17.13731285436883	32.525142802151954	32.21719373502934	18.120350608449883
18	17.149645396616535	28.103257551885218	38.83167447422583	15.915422577272423
19	22.782497030232953	26.400536865323538	29.77084341384116	21.04612269060235
20	23.29079409364258	29.17658289350335	31.79934523236604	15.73327778048803
21	20.30681851141868	27.580837818097443	28.62316643243058	23.489177238053298
22	21.71870529492658	33.34251881815772	29.048326637598343	15.890449249317351
23	17.662710619619553	32.81637937968163	32.243607408589206	17.277302592109617
24	21.992162984605486	27.988323585408175	33.54015811152189	16.47935531846445
25	19.1716143289045	30.912835442270243	32.981030775052446	16.934519453772815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	134.0
2	386.0
3	638.0
4	638.0
5	638.0
6	1974.0
7	3310.0
8	3310.0
9	3310.0
10	3440.5
11	3571.0
12	3571.0
13	3571.0
14	3925.0
15	4279.0
16	4279.0
17	4279.0
18	7933.5
19	11588.0
20	11588.0
21	11588.0
22	20021.0
23	28454.0
24	28454.0
25	28454.0
26	44667.0
27	60880.0
28	60880.0
29	60880.0
30	80908.0
31	100936.0
32	100936.0
33	100936.0
34	105629.0
35	110322.0
36	110322.0
37	110322.0
38	108969.5
39	107617.0
40	107617.0
41	107617.0
42	104747.0
43	101877.0
44	101877.0
45	101877.0
46	98870.0
47	95863.0
48	95863.0
49	95863.0
50	81777.0
51	67691.0
52	67691.0
53	67691.0
54	49175.0
55	30659.0
56	30659.0
57	30659.0
58	23120.5
59	15582.0
60	15582.0
61	15582.0
62	11737.0
63	7892.0
64	7892.0
65	7892.0
66	5990.5
67	4089.0
68	4089.0
69	4089.0
70	2945.5
71	1802.0
72	1802.0
73	1802.0
74	1333.0
75	864.0
76	864.0
77	864.0
78	640.5
79	417.0
80	417.0
81	417.0
82	283.0
83	149.0
84	149.0
85	149.0
86	105.0
87	61.0
88	61.0
89	61.0
90	53.5
91	46.0
92	46.0
93	46.0
94	36.5
95	27.0
96	27.0
97	27.0
98	178.5
99	330.0
100	330.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038731197584437965
2	0.005269550691760267
3	0.0018443427421160933
4	0.004610856855290234
5	0.011197795219990567
6	0.016072129609868813
7	0.02700644729527137
8	0.04228814430137614
9	0.054408110892424755
10	0.06468373474135727
11	0.062048959395477145
12	0.06837242022558947
13	0.07008502420041156
14	0.07364197091734974
15	0.06665981625076738
16	0.07127067310605761
17	0.06731851008723741
18	0.08233672955875418
19	0.07917499914369802
20	0.08444454983545828
21	0.07693544009969991
22	0.08365411723169423
23	0.0870793251813384
24	0.08246846832604818
25	0.08418107230087027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	759078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34309149395441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.30631765056579	41.51137840832176
2	12.905928093354625	13.510723500097152
3	3.628849878206654	5.6983566357838855
4	1.4039425374015095	2.9394677074984688
5	0.7063105393948214	1.848523859334371
6	0.43843851465304085	1.3769536372174553
7	0.26765391392100074	0.9806883313557354
8	0.17838956094965908	0.7469968888283489
9	0.15038843313950057	0.7084615963907991
>10	0.8460463474702554	8.335934057696184
>50	0.08071445213204045	2.9626703508024073
>100	0.07138168783653748	7.6853704584215325
>500	0.00832365971231353	3.072379033810014
>1k	0.006558034924853085	6.456603681382189
>5k	7.566963374830482E-4	2.1654918530596814
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	5939	0.7823965389591057	No Hit
GTCCTACAGTGGACATTTCTAAATT	5416	0.7134971636643401	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5047	0.6648855585328517	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4753	0.6261543609484137	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4093	0.5392067745343694	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3595	0.47360086842195404	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2794	0.36807811581945465	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2553	0.336329072901599	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2538	0.33435299139218894	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2329	0.30681958902774153	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2250	0.29641222641151505	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2088	0.27507054610988596	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1832	0.24134542168262021	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1699	0.22382416563251734	No Hit
GAATATGGCAAGAAAACTGAAAATC	1663	0.21908157000993309	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1539	0.2027459628654763	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1416	0.18654209448831346	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1379	0.18166776009843522	No Hit
CTGTAGGACATGGAATATGGCAAGA	1240	0.1633560714445683	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1192	0.15703261061445598	No Hit
GATATACACTGTTCTACAAATCCCG	1166	0.1536074026648118	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1154	0.1520265374572837	No Hit
TTGTAGAACAGTGTATATCAATGAG	1153	0.1518947986899897	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1127	0.14846959074034552	No Hit
GCCATATTCCACGTCCTACAGTGGA	1110	0.1462300316963474	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1099	0.14478090525611334	No Hit
GTACATGGGGTGGTATCAACGCAAA	1055	0.13898439949517705	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1051	0.13845744442600103	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1036	0.1364813629165909	No Hit
GACCTGGAATATGGCGAGAAAACTG	997	0.13134355099212466	No Hit
ACAGTGGACATTTCTAAATTTTCCA	958	0.1262057390676584	No Hit
GTCCACTGTAGGACGTGGAATATGG	938	0.12357096372177827	No Hit
GTGTATATCAATGAGTTACAATGAA	934	0.12304400865260225	No Hit
ATACACACTTTAGGACGTGAAATAT	895	0.11790619672813597	No Hit
GAAATACACACTTTAGGACGTGAAA	871	0.11474446631307982	No Hit
CCACTGTAGGACGTGGAATATGGCA	841	0.11079230329425961	No Hit
GTGTATTTCTCATTTTCCGTGATTT	835	0.11000187069049557	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	794	0.10460058123144131	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	790	0.10407362616226529	No Hit
CACTTTAGGACGTGAAATATGGCGA	776	0.1022292834201492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.3173876729400668E-4	0.0	0.0	0.0
5	0.0	1.3173876729400668E-4	0.0	0.0	0.0
6	0.0	1.3173876729400668E-4	0.0	0.0	0.0
7	0.0	1.3173876729400668E-4	0.0	0.0	0.0
8	0.0	1.3173876729400668E-4	0.0	0.0	0.0
9	0.0	1.3173876729400668E-4	0.0	0.0	0.0
10	0.0	1.3173876729400668E-4	0.0	0.0	0.0
11	0.0	1.3173876729400668E-4	0.0	1.3173876729400668E-4	0.0
12	0.0	1.3173876729400668E-4	0.0	1.3173876729400668E-4	0.0
13	0.0	2.6347753458801337E-4	0.0	1.3173876729400668E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGTG	35	9.937188E-5	18.998484	6
TTTCGAA	40	2.756297E-4	16.626963	16
GTCCTAG	160	0.0	15.43525	1
TCGAAAT	50	8.6958076E-5	15.201795	18
AATGGCG	65	3.3625056E-6	14.617109	16
GTCTTAC	85	1.715307E-8	14.527294	1
TCCTAGA	170	0.0	14.523466	2
CCAACGT	60	2.5619898E-5	14.25168	19
CGTGTGC	40	0.0052739847	14.250741	8
CAGTCCC	40	0.0052739847	14.250741	9
CCAAGGC	40	0.005278601	14.248862	3
AGTAGTG	40	0.005278601	14.248862	5
GATCTAG	40	0.00528091	14.247924	1
GTGTAGC	75	9.669802E-7	13.931303	1
TTCGAAA	55	1.9572055E-4	13.818902	17
CCAACGA	145	0.0	13.760244	19
TAGGACC	760	0.0	13.248943	4
GACTGTT	65	5.4426513E-5	13.1545315	7
TGGACTG	65	5.4493903E-5	13.152797	5
TCCAACG	210	0.0	13.120595	18
>>END_MODULE