FastQCFastQC Report
Thu 2 Feb 2017
SRR4064232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759078
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA26400.3477903456561776No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA22170.2920648470908128No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA19940.2626871019842493No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19460.25636364115413696No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13050.1719190913186787No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11500.15149958238810768No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9970.13134355099212466No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA9360.12330748618719024No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA8020.10565449136979335No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA7920.10433710369685328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.1022292834201492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTT300.00573696129.34036430
AGCGGAC454.0718965E-529.3287668
TAGGACC12050.029.0245274
TCGGAAA554.9562404E-628.00117338
GGACGTA554.9638675E-627.9956426
CTCGGAA609.743566E-625.66774437
GCGGACA551.5931153E-423.9994269
CAGCGGA551.5945396E-423.9962647
ACAGCGG551.5945396E-423.9962646
AGGACCT22150.023.3372235
GGCGAAA852.3522989E-723.29202519
GCGAAAA952.6826456E-823.1573420
ATAGGAC2550.022.4278813
GGACCTG22250.022.2437296
TTACTCG500.00258090721.99802443
TGGCGAA1500.021.99802218
ACGGGAT1407.2759576E-1221.99802243
CTAGGAC1700.021.9965743
TATGTCG1950.021.43397116
TGTAGGA36950.021.3119722
Produced by FastQC (version 0.11.5)