FastQCFastQC Report
Thu 2 Feb 2017
SRR4064231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710597
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54810.32041445179665345No Hit
TATCAACGCAGAGTACTTTTTTTTT35160.205542275591504No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29280.17116831141408526No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19750.11545676743265655No Hit
GGTATCAACGCAGAGTACTTTTTTT18780.10978623252583747No Hit
GTACATGGGGTGGTATCAACGCAAA17620.10300497428675487No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17460.10206962832274345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG350.00216931516.28684216
TCGAATT350.00217016316.2858914
GCGTTAT1500.015.1979411
CGTTATT1400.014.2455692
CGCATCG2050.013.90380913
GCATCGC2450.012.40829714
GTATAGA853.949941E-612.2924521
CCCCTAT658.0332684E-411.6907231
TAAGACC907.477798E-611.6088594
TCCTATC907.4875425E-611.6075012
GTACTAA752.0766577E-411.3984561
CGAGCCG2850.011.33411915
GTTTTCG1604.5474735E-1111.28203215
GTTATTC2450.011.2417313
GTCCTAT3050.011.21159651
TATTCCC2300.011.1503375
CGGAATT600.005873037511.08410115
CGCAGTT600.005873037511.08410117
TGGCGAG4300.011.0485718
CCAACGA1553.110472E-1011.034636519
Produced by FastQC (version 0.11.5)