##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064231_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1710597
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.185623498696653	32.0	32.0	32.0	32.0	32.0
2	30.76176446001016	32.0	32.0	32.0	32.0	32.0
3	30.78135528122638	32.0	32.0	32.0	32.0	32.0
4	30.81339029590254	32.0	32.0	32.0	32.0	32.0
5	30.722102868179938	32.0	32.0	32.0	32.0	32.0
6	34.36066823454034	36.0	36.0	36.0	32.0	36.0
7	34.28440889350326	36.0	36.0	36.0	32.0	36.0
8	34.25109187026518	36.0	36.0	36.0	32.0	36.0
9	34.397752948239706	36.0	36.0	36.0	32.0	36.0
10	34.08636984631681	36.0	36.0	36.0	32.0	36.0
11	34.38147792846591	36.0	36.0	36.0	32.0	36.0
12	34.1863852210661	36.0	36.0	36.0	32.0	36.0
13	34.26702899630948	36.0	36.0	36.0	32.0	36.0
14	34.17466592072826	36.0	36.0	36.0	32.0	36.0
15	34.10959331742076	36.0	36.0	36.0	32.0	36.0
16	34.10732510345803	36.0	36.0	36.0	32.0	36.0
17	34.03502578339609	36.0	36.0	36.0	32.0	36.0
18	34.02688710432673	36.0	36.0	36.0	32.0	36.0
19	34.04460723361493	36.0	36.0	36.0	32.0	36.0
20	34.00765171457684	36.0	36.0	36.0	32.0	36.0
21	33.99524435036423	36.0	36.0	36.0	32.0	36.0
22	33.97379628281822	36.0	36.0	36.0	32.0	36.0
23	33.929373780031185	36.0	36.0	36.0	32.0	36.0
24	33.90856876283543	36.0	36.0	36.0	32.0	36.0
25	33.501768096167595	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	15.0
5	28.0
6	78.0
7	31.0
8	79.0
9	122.0
10	131.0
11	59.0
12	92.0
13	84.0
14	183.0
15	346.0
16	565.0
17	736.0
18	973.0
19	1402.0
20	2233.0
21	3274.0
22	4993.0
23	7661.0
24	10961.0
25	15530.0
26	21143.0
27	27517.0
28	36803.0
29	48135.0
30	62861.0
31	83575.0
32	116692.0
33	163202.0
34	346904.0
35	754181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.446405857532596	17.17416159093767	11.426106840318493	25.953325711211246
2	16.31595009172767	20.146530425642123	37.810216673740634	25.727302808889576
3	18.862656490691673	24.533239017517605	28.57172922396986	28.032375267820864
4	12.596147494070529	15.746061292364965	36.13941467024998	35.51837654331453
5	14.406987261075743	36.833141665794564	33.652941633872715	15.10692943925698
6	33.42968748629672	35.678691641569564	17.299780104387562	13.591840767746149
7	29.44802136367065	30.66846841262945	21.298590276068257	18.584919947631644
8	27.67506160825919	33.093255079830506	19.844643093149816	19.38704021876049
9	27.700520122050005	14.098565263239776	18.598004083540033	39.60291053117018
10	15.83319831331235	27.01823735408333	31.84854204698931	25.300022285615015
11	36.74074481351586	21.31044269072481	22.941257671223582	19.00755482453575
12	24.879453550058585	23.630379986744718	29.27153851958489	22.218627943611814
13	29.72160183071716	19.862661114926404	25.239573815409706	25.17616323894673
14	23.508266171844884	19.813482450654806	25.581755864650173	31.096495512850137
15	24.874530880026953	27.39262885005943	22.622053084259093	25.11078718565452
16	25.101714775098465	25.801711323669725	24.388439000676833	24.708134900554978
17	23.608793237483045	25.880806602474	25.543464590787817	24.96693556925514
18	24.26419311054947	25.11250351024993	26.692349995319663	23.930953383880933
19	25.417474978412518	24.86374730595253	25.659617486210923	24.05916022942403
20	25.667090439028556	24.5536202699311	25.29874196358522	24.480547327455124
21	26.620944165934883	24.231833320950074	24.742569606101224	24.40465290701382
22	25.631842030131846	24.627505721680606	25.114784635473953	24.6258676127136
23	24.36757628342318	24.507289298380897	25.738320097260853	25.38681432093507
24	24.689980623057625	25.110925974463623	25.628405006178156	24.570688396300593
25	24.71148907092636	24.79631941608641	25.691425816740715	24.800765696246522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	239.0
1	239.0
2	575.0
3	911.0
4	911.0
5	911.0
6	2441.5
7	3972.0
8	3972.0
9	3972.0
10	4072.0
11	4172.0
12	4172.0
13	4172.0
14	4476.0
15	4780.0
16	4780.0
17	4780.0
18	8275.0
19	11770.0
20	11770.0
21	11770.0
22	18899.0
23	26028.0
24	26028.0
25	26028.0
26	38749.5
27	51471.0
28	51471.0
29	51471.0
30	65909.0
31	80347.0
32	80347.0
33	80347.0
34	95761.0
35	111175.0
36	111175.0
37	111175.0
38	126511.5
39	141848.0
40	141848.0
41	141848.0
42	161400.5
43	180953.0
44	180953.0
45	180953.0
46	201925.5
47	222898.0
48	222898.0
49	222898.0
50	227249.0
51	231600.0
52	231600.0
53	231600.0
54	215196.0
55	198792.0
56	198792.0
57	198792.0
58	181066.0
59	163340.0
60	163340.0
61	163340.0
62	142416.5
63	121493.0
64	121493.0
65	121493.0
66	99229.5
67	76966.0
68	76966.0
69	76966.0
70	57656.5
71	38347.0
72	38347.0
73	38347.0
74	28937.5
75	19528.0
76	19528.0
77	19528.0
78	15348.0
79	11168.0
80	11168.0
81	11168.0
82	7722.0
83	4276.0
84	4276.0
85	4276.0
86	3274.5
87	2273.0
88	2273.0
89	2273.0
90	1607.5
91	942.0
92	942.0
93	942.0
94	659.0
95	376.0
96	376.0
97	376.0
98	654.0
99	932.0
100	932.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03869993926097146
2	0.005787453152320506
3	0.0018706919280227898
4	0.004325975083552701
5	0.00993805086762107
6	0.014030189460170924
7	0.023617485591287717
8	0.035017014527676596
9	0.047118052937074016
10	0.05693918555919367
11	0.0561792169634344
12	0.06372044379827628
13	0.06448041239403553
14	0.06781258239082613
15	0.059219091346471436
16	0.06781258239082613
17	0.061732833624752056
18	0.07699066466268795
19	0.0744184632616566
20	0.0786859792224586
21	0.07599685957592583
22	0.07652299168068225
23	0.0806151302732321
24	0.07886135659071072
25	0.07617223694417798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1710597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.28869879308079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9231078460185	39.69920517595049
2	13.957176959923101	14.596052441182922
3	4.420749914490093	6.934657821549303
4	1.9160939596050117	4.0076023965211185
5	1.0020188674329422	2.6197131372092533
6	0.621111340047198	1.9486262266016832
7	0.3988532956222433	1.4598863886193358
8	0.2857946238262027	1.1955063201544114
9	0.2172159256875903	1.0222144300204765
>10	1.0905567731778687	10.262950136349733
>50	0.08285176913178041	3.0254431696059116
>100	0.07346121363602016	8.006914291015411
>500	0.008536437413408713	3.017094566236328
>1k	0.0023587524431787233	1.8822262883483258
>5k	1.1232154491327253E-4	0.32190721063520883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5481	0.32041445179665345	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3516	0.205542275591504	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2928	0.17116831141408526	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1975	0.11545676743265655	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1878	0.10978623252583747	No Hit
GTACATGGGGTGGTATCAACGCAAA	1762	0.10300497428675487	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1746	0.10206962832274345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3383649100284872E-4
2	0.0	0.0	0.0	0.0	2.3383649100284872E-4
3	0.0	0.0	0.0	0.0	2.3383649100284872E-4
4	0.0	0.0	0.0	0.0	2.3383649100284872E-4
5	0.0	0.0	0.0	0.0	2.3383649100284872E-4
6	0.0	0.0	0.0	0.0	2.9229561375356087E-4
7	0.0	0.0	0.0	5.845912275071218E-5	2.9229561375356087E-4
8	0.0	0.0	0.0	5.845912275071218E-5	2.9229561375356087E-4
9	0.0	0.0	0.0	5.845912275071218E-5	2.9229561375356087E-4
10	0.0	0.0	0.0	2.3383649100284872E-4	2.9229561375356087E-4
11	0.0	0.0	0.0	2.3383649100284872E-4	3.507547365042731E-4
12	0.0	0.0	0.0	2.9229561375356087E-4	4.6767298200569744E-4
13	0.0	0.0	0.0	2.9229561375356087E-4	5.261321047564096E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGCG	35	0.002169315	16.286842	16
TCGAATT	35	0.002170163	16.28589	14
GCGTTAT	150	0.0	15.197941	1
CGTTATT	140	0.0	14.245569	2
CGCATCG	205	0.0	13.903809	13
GCATCGC	245	0.0	12.408297	14
GTATAGA	85	3.949941E-6	12.292452	1
CCCCTAT	65	8.0332684E-4	11.690723	1
TAAGACC	90	7.477798E-6	11.608859	4
TCCTATC	90	7.4875425E-6	11.607501	2
GTACTAA	75	2.0766577E-4	11.398456	1
CGAGCCG	285	0.0	11.334119	15
GTTTTCG	160	4.5474735E-11	11.282032	15
GTTATTC	245	0.0	11.241731	3
GTCCTAT	305	0.0	11.2115965	1
TATTCCC	230	0.0	11.150337	5
CGGAATT	60	0.0058730375	11.084101	15
CGCAGTT	60	0.0058730375	11.084101	17
TGGCGAG	430	0.0	11.04857	18
CCAACGA	155	3.110472E-10	11.0346365	19
>>END_MODULE