FastQCFastQC Report
Thu 2 Feb 2017
SRR4064231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710597
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA551.5951521E-423.9974357
TAGGACG4300.021.4860744
TACTAGT755.478847E-520.5311372
GTAGGAC7300.019.8882833
GTATCAA42850.018.841011
TCGTAAT600.007415403618.33244312
AACGCAG45700.017.8580466
TTAGGAC4400.017.4981293
TACCGTC4050.017.3808577
GTAATAC1408.3882696E-817.2838653
CGTCGTA3950.017.26498610
ATACACT5450.016.9523164
GTACTAG1307.3271076E-716.9217621
TATGGCG6800.016.8217316
CCGTCGT3850.016.5706259
TGGCGAG7600.016.49823618
ATACCGT4050.016.2945546
ACCGTCG3650.016.2722328
TAGGACC10600.016.1869474
GTCTAAA2051.05501385E-1016.096311