FastQCFastQC Report
Thu 2 Feb 2017
SRR4064230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614600
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23410.38089814513504716No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16760.2726976895541816No Hit
TATCAACGCAGAGTACTTTTTTTTT16700.27172144484217375No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11740.19101854864952816No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9120.1483891962251871No Hit
GGTATCAACGCAGAGTACTTTTTTT7960.12951513179303611No Hit
GTACATGGGGTGGTATCAACGCAAA7730.12577286039700616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC359.917399E-519.00244317
ATTTGCG359.917399E-519.00244316
GTAAACG402.7550044E-416.62713617
AACGTCT456.7558506E-414.7772716
CGAACGT456.7558506E-414.7772714
GTAATTT456.7558506E-414.77727113
CTTGTAC456.764196E-414.7748663
TCGAACG456.764196E-414.7748663
GCGTTAT456.7683717E-414.7736641
TGTTCAA653.3624638E-614.61607410
AGAACCG602.5641037E-514.2495135
CGAAGAC602.5641037E-514.2495134
GGATTAT400.00528404914.2460331
AGACGAT551.9563339E-413.8188337
AGTTAAA903.6185156E-813.72398717
CGAGCCG1051.3606041E-913.57317315
TAGAGTG852.6911584E-713.4113045
TAAACGC500.001496297513.30170918
TTGCGCG500.001496297513.30170918
ACGTCTG500.001497215913.3006267
Produced by FastQC (version 0.11.5)