##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064230_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614600
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14994630654084	32.0	32.0	32.0	32.0	32.0
2	30.744536283761796	32.0	32.0	32.0	32.0	32.0
3	30.761688903351775	32.0	32.0	32.0	32.0	32.0
4	30.79761958997722	32.0	32.0	32.0	32.0	32.0
5	30.685676863000324	32.0	32.0	32.0	32.0	32.0
6	34.32287178652782	36.0	36.0	36.0	32.0	36.0
7	34.24095509274325	36.0	36.0	36.0	32.0	36.0
8	34.170561340709405	36.0	36.0	36.0	32.0	36.0
9	34.34624471200781	36.0	36.0	36.0	32.0	36.0
10	34.01602180279857	36.0	36.0	36.0	32.0	36.0
11	34.33723071916694	36.0	36.0	36.0	32.0	36.0
12	34.13857630979499	36.0	36.0	36.0	32.0	36.0
13	34.23223885453954	36.0	36.0	36.0	32.0	36.0
14	34.11682883176049	36.0	36.0	36.0	32.0	36.0
15	34.0501871135698	36.0	36.0	36.0	32.0	36.0
16	34.05341197526847	36.0	36.0	36.0	32.0	36.0
17	33.97420436055971	36.0	36.0	36.0	32.0	36.0
18	33.9643735763098	36.0	36.0	36.0	32.0	36.0
19	33.98105109013993	36.0	36.0	36.0	32.0	36.0
20	33.95664497233973	36.0	36.0	36.0	32.0	36.0
21	33.92719817767654	36.0	36.0	36.0	32.0	36.0
22	33.90636674259681	36.0	36.0	36.0	32.0	36.0
23	33.869448421737715	36.0	36.0	36.0	32.0	36.0
24	33.84490562967784	36.0	36.0	36.0	32.0	36.0
25	33.44526521314676	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	10.0
6	22.0
7	13.0
8	39.0
9	42.0
10	65.0
11	17.0
12	44.0
13	29.0
14	73.0
15	130.0
16	213.0
17	269.0
18	390.0
19	476.0
20	700.0
21	1080.0
22	1630.0
23	2467.0
24	3769.0
25	5407.0
26	7377.0
27	9961.0
28	13679.0
29	18300.0
30	23808.0
31	32381.0
32	45281.0
33	62798.0
34	128764.0
35	255361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54839003714422	17.24406782971603	11.290737382845945	25.9168047502938
2	16.58091453750801	20.124413810160732	37.48718599587999	25.807485656451263
3	18.796982694097508	24.624952000364473	28.503322551042327	28.074742754495695
4	12.523186868259536	15.327414851311156	36.111309984834975	36.038088295594335
5	14.434791381563212	36.95380144269807	33.525562897958324	15.0858442777804
6	33.350365652430035	35.75029535343147	17.263123293377898	13.636215700760596
7	29.707490222820415	30.69896051858518	21.252976282507188	18.34057297608721
8	28.09845789235522	32.52636102312347	19.93313301451606	19.442048070005242
9	27.76182429682591	13.854133091100888	18.60436343983096	39.77967917224225
10	15.68936464650576	26.710244617200534	32.082705848833896	25.517684887459808
11	36.899798284358816	21.218889961594254	22.849709636928125	19.031602117118805
12	24.770549590107215	23.678858975715787	29.262030170062765	22.288561264114232
13	29.67153379746918	19.565507629585728	25.553739245963776	25.209219326981312
14	23.639318157024483	19.583039571185964	25.722492058245507	31.055150213544046
15	24.894903597862577	27.320505765241826	22.8467041572913	24.937886479604295
16	25.187855459979257	25.69113713000819	24.35127790559276	24.769729504419793
17	23.547684908557184	25.946868248430093	25.638015724289286	24.86743111872343
18	24.21607468235087	25.00858951249021	26.865715388335527	23.909620416823397
19	25.404116805103914	24.77264104397045	25.91981658218222	23.903425568743415
20	25.591108939912065	24.169842045269498	25.47907506920697	24.759973945611463
21	26.512049006948185	24.044278207572827	24.985304103289764	24.458368682189224
22	25.64520515154525	24.381581711330437	25.357144601836488	24.616068535287823
23	24.243011474112407	24.411556838056402	25.874563166242726	25.470868521588464
24	24.596931123707662	24.9369403404326	25.87929347126938	24.58683506459035
25	24.70839202403537	24.603522256328418	25.834113614343057	24.853972105293153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	328.5
3	454.0
4	454.0
5	454.0
6	1292.5
7	2131.0
8	2131.0
9	2131.0
10	2080.5
11	2030.0
12	2030.0
13	2030.0
14	1916.0
15	1802.0
16	1802.0
17	1802.0
18	3080.5
19	4359.0
20	4359.0
21	4359.0
22	6888.5
23	9418.0
24	9418.0
25	9418.0
26	13859.0
27	18300.0
28	18300.0
29	18300.0
30	23113.0
31	27926.0
32	27926.0
33	27926.0
34	33540.5
35	39155.0
36	39155.0
37	39155.0
38	44541.0
39	49927.0
40	49927.0
41	49927.0
42	57123.0
43	64319.0
44	64319.0
45	64319.0
46	72025.0
47	79731.0
48	79731.0
49	79731.0
50	81669.0
51	83607.0
52	83607.0
53	83607.0
54	77196.5
55	70786.0
56	70786.0
57	70786.0
58	64799.5
59	58813.0
60	58813.0
61	58813.0
62	51192.5
63	43572.0
64	43572.0
65	43572.0
66	35801.0
67	28030.0
68	28030.0
69	28030.0
70	21322.5
71	14615.0
72	14615.0
73	14615.0
74	11158.0
75	7701.0
76	7701.0
77	7701.0
78	6023.0
79	4345.0
80	4345.0
81	4345.0
82	2969.5
83	1594.0
84	1594.0
85	1594.0
86	1238.5
87	883.0
88	883.0
89	883.0
90	630.5
91	378.0
92	378.0
93	378.0
94	270.5
95	163.0
96	163.0
97	163.0
98	260.5
99	358.0
100	358.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0387243735763098
2	0.006182883176049463
3	0.0019524894240156199
4	0.004555808656036447
5	0.009274324764074193
6	0.013342011064106736
7	0.02587048486820696
8	0.04002603319232021
9	0.051090139928408725
10	0.06101529450048812
11	0.06003904978848032
12	0.06752359258054019
13	0.06703547022453628
14	0.07175398633257403
15	0.06540839570452327
16	0.07012691181256102
17	0.06361861373250895
18	0.0779368695086235
19	0.07858769931662869
20	0.08135372600065083
21	0.07858769931662869
22	0.08021477383664172
23	0.08493328994467947
24	0.08119101854864953
25	0.08216726326065735
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	614600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.79677795460131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03878826172908	46.47226083225633
2	12.977370266551791	15.260551159941818
3	3.7082945747090923	6.541073180982695
4	1.4746778905946885	3.4682523395142293
5	0.7631239198320894	2.2434613833106165
6	0.4473038107804955	1.5780013704424678
7	0.2865923052171142	1.179549289334359
8	0.2063043972266325	0.970402706783374
9	0.1443960945897591	0.7641022599995105
>10	0.7972029060419668	8.603056850706144
>50	0.08296750250559136	3.44346951367523
>100	0.0702032386543702	7.69420849469067
>500	0.0016648989404182515	0.662230540286954
>1k	0.0011099326269455008	1.1193800780755832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2341	0.38089814513504716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1676	0.2726976895541816	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1670	0.27172144484217375	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1174	0.19101854864952816	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	912	0.1483891962251871	No Hit
GGTATCAACGCAGAGTACTTTTTTT	796	0.12951513179303611	No Hit
GTACATGGGGTGGTATCAACGCAAA	773	0.12577286039700616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6270745200130165E-4	0.0
5	0.0	0.0	0.0	1.6270745200130165E-4	0.0
6	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
7	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
8	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
9	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
10	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
11	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
12	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
13	0.0	0.0	0.0	1.6270745200130165E-4	3.254149040026033E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGC	35	9.917399E-5	19.002443	17
ATTTGCG	35	9.917399E-5	19.002443	16
GTAAACG	40	2.7550044E-4	16.627136	17
AACGTCT	45	6.7558506E-4	14.777271	6
CGAACGT	45	6.7558506E-4	14.777271	4
GTAATTT	45	6.7558506E-4	14.777271	13
CTTGTAC	45	6.764196E-4	14.774866	3
TCGAACG	45	6.764196E-4	14.774866	3
GCGTTAT	45	6.7683717E-4	14.773664	1
TGTTCAA	65	3.3624638E-6	14.616074	10
AGAACCG	60	2.5641037E-5	14.249513	5
CGAAGAC	60	2.5641037E-5	14.249513	4
GGATTAT	40	0.005284049	14.246033	1
AGACGAT	55	1.9563339E-4	13.818833	7
AGTTAAA	90	3.6185156E-8	13.723987	17
CGAGCCG	105	1.3606041E-9	13.573173	15
TAGAGTG	85	2.6911584E-7	13.411304	5
TAAACGC	50	0.0014962975	13.301709	18
TTGCGCG	50	0.0014962975	13.301709	18
ACGTCTG	50	0.0014972159	13.300626	7
>>END_MODULE