FastQCFastQC Report
Thu 2 Feb 2017
SRR4064230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614600
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.10608525870484868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC1051.1277734E-1025.1436027
CTATTAG450.001396529124.4471571
GTCCTAA1153.4924597E-1022.959071
TCTAATC803.60983E-622.0006523
ACCGTCG1205.966285E-1021.998868
TGGTCGG1451.2732926E-1121.24200837
ATACCGT1259.931682E-1021.1206256
GTATTAC550.00447827120.0022181
GTTATAT550.00447827120.0022181
CGTTTAT1452.9467628E-1019.72311631
GATATAC1051.7878647E-618.8592361
CTAGTAC708.115504E-418.8577023
TCCTACA1759.094947E-1218.85772
CGGCATC1658.185452E-1118.6672225
GACAGTC951.5946223E-518.5268657
TAGCGTA851.4290598E-418.1181837
CGTCGTA1355.5157216E-817.92499710
TAGGACA1601.0968506E-917.875534
TCGTTTA1601.0986696E-917.87407530
CATCGTT1852.1827873E-1117.83691428
Produced by FastQC (version 0.11.5)