##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614600
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15026684022128	32.0	32.0	32.0	32.0	32.0
2	31.272959648551904	32.0	32.0	32.0	32.0	32.0
3	31.391480637813213	32.0	32.0	32.0	32.0	32.0
4	31.502993817116824	32.0	32.0	32.0	32.0	32.0
5	31.41482753010088	32.0	32.0	32.0	32.0	32.0
6	35.004770582492675	36.0	36.0	36.0	36.0	36.0
7	35.02774324764074	36.0	36.0	36.0	36.0	36.0
8	34.968496583143505	36.0	36.0	36.0	36.0	36.0
9	35.093434754311744	36.0	36.0	36.0	36.0	36.0
10	34.922806703547025	36.0	36.0	36.0	32.0	36.0
11	35.12133094695737	36.0	36.0	36.0	36.0	36.0
12	34.99637162382037	36.0	36.0	36.0	36.0	36.0
13	35.04770094370322	36.0	36.0	36.0	36.0	36.0
14	34.988060527172145	36.0	36.0	36.0	36.0	36.0
15	34.94901236576635	36.0	36.0	36.0	36.0	36.0
16	34.97007484542792	36.0	36.0	36.0	36.0	36.0
17	34.926609176700296	36.0	36.0	36.0	36.0	36.0
18	34.92206638464042	36.0	36.0	36.0	36.0	36.0
19	34.929700618288315	36.0	36.0	36.0	36.0	36.0
20	34.91389033517735	36.0	36.0	36.0	32.0	36.0
21	34.918462414578585	36.0	36.0	36.0	32.0	36.0
22	34.89404978848031	36.0	36.0	36.0	32.0	36.0
23	34.838509599739666	36.0	36.0	36.0	32.0	36.0
24	34.81783110966482	36.0	36.0	36.0	32.0	36.0
25	34.79676700292873	36.0	36.0	36.0	32.0	36.0
26	34.72327692808331	36.0	36.0	36.0	32.0	36.0
27	34.72795476732834	36.0	36.0	36.0	32.0	36.0
28	34.7047396680768	36.0	36.0	36.0	32.0	36.0
29	34.66475105759844	36.0	36.0	36.0	32.0	36.0
30	34.64496257728604	36.0	36.0	36.0	32.0	36.0
31	34.631900423039376	36.0	36.0	36.0	32.0	36.0
32	34.57861861373251	36.0	36.0	36.0	32.0	36.0
33	34.55176537585422	36.0	36.0	36.0	32.0	36.0
34	34.53376830458835	36.0	36.0	36.0	32.0	36.0
35	34.49013667425968	36.0	36.0	36.0	32.0	36.0
36	34.44751871135698	36.0	36.0	36.0	32.0	36.0
37	34.43943703221608	36.0	36.0	36.0	32.0	36.0
38	34.400569476082005	36.0	36.0	36.0	32.0	36.0
39	34.3874227139603	36.0	36.0	36.0	32.0	36.0
40	34.35108525870485	36.0	36.0	36.0	32.0	36.0
41	34.330553205336805	36.0	36.0	36.0	32.0	36.0
42	34.27368206963879	36.0	36.0	36.0	32.0	36.0
43	34.24905629677839	36.0	36.0	36.0	32.0	36.0
44	34.16388219980475	36.0	36.0	36.0	32.0	36.0
45	34.117948259030264	36.0	36.0	36.0	32.0	36.0
46	34.08629352424341	36.0	36.0	36.0	32.0	36.0
47	34.03397006182883	36.0	36.0	36.0	32.0	36.0
48	33.97889684347543	36.0	36.0	36.0	32.0	36.0
49	33.95875854214123	36.0	36.0	36.0	32.0	36.0
50	33.326954116498534	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	15.0
22	65.0
23	199.0
24	536.0
25	1229.0
26	2589.0
27	4767.0
28	8073.0
29	12027.0
30	17100.0
31	24715.0
32	35382.0
33	56772.0
34	114473.0
35	336651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59715367579697	18.266788382336905	11.917233344235672	26.21882459763045
2	15.844314748315568	21.027206357315908	36.942787085563104	26.185691808805416
3	18.061858219749787	25.189920582363463	28.940658868628162	27.80756232925859
4	12.208428246013668	16.04881223560039	36.28246013667426	35.460299381711685
5	14.053205336804425	37.35112268141881	33.30507647250244	15.290595509274324
6	33.488122356003906	36.219329645297755	16.940611780019523	13.351936218678814
7	29.438869897282615	30.887456699408883	21.291118273866374	18.382555129442125
8	28.063130491376505	32.79645297754637	19.69720143182558	19.443215099251546
9	27.884984607774864	13.699568497028944	18.425111780747027	39.99033511444917
10	15.569678746225138	26.94372852233677	32.171912423201086	25.31468030823701
11	37.178001952489424	21.16108037748129	22.699967458509597	18.960950211519688
12	24.692808330621542	23.9123006833713	29.04848682069639	22.34640416531077
13	29.752992611418538	19.568920772473668	25.409007853927047	25.269078762180747
14	23.515820884837105	19.5587692137475	25.76135008355041	31.16405981786498
15	25.03050893789845	27.370666510460907	22.752996384487467	24.845828167153183
16	25.26456403881574	25.68955866943488	24.18011181328873	24.86576547846065
17	23.52375528799219	25.90872111942727	25.490562967783926	25.07696062479662
18	24.329359468531514	24.96001457861142	26.858488217520694	23.852137735336374
19	25.411569839146892	24.74218920334918	26.06484238477834	23.781398572725585
20	25.775673410945423	24.05047226222146	25.571636606220356	24.602217720612764
21	26.680930686625448	24.05711031565246	25.052229092092414	24.20972990562968
22	25.792222583794338	24.350959973966805	25.29238529124634	24.564432150992516
23	24.309262817748866	24.180072470252863	26.02599752358049	25.484667188417774
24	24.75817486906054	24.899242918530355	25.883139683402295	24.45944252900681
25	24.73946002938549	24.429659496020918	26.031705741560618	24.79917473303297
26	24.4773928791678	25.113617318322124	26.193083540662226	24.21590626184785
27	24.916526730972	24.937842517020087	25.55616302833861	24.58946772366931
28	24.16173778627507	24.851157303827847	26.090875808485535	24.896229101411542
29	24.279319810020617	24.87581212281949	26.001760514682076	24.843107552477818
30	24.23016656876799	25.16234637907778	26.141061959286084	24.46642509286815
31	24.719026163476574	24.915752596577384	25.370875701112507	24.99434553883354
32	24.456054827330355	25.16856930867058	25.307525090142274	25.067850773856787
33	24.196464165710708	24.664274730907227	25.890345203517935	25.248915899864134
34	24.809221166769174	24.72526220903177	26.133039151558933	24.332477472640118
35	25.339054341441347	24.6167689321273	25.924985803243533	24.11919092318782
36	24.415632174930398	25.22158381783182	25.539213054040903	24.823570953196878
37	25.144164432063732	25.310457941735443	25.28149488427794	24.263882741922888
38	24.613134077721405	25.035879678596185	25.52436401032293	24.826622233359476
39	24.942236598890943	25.072895785061572	25.188422587279685	24.7964450287678
40	25.15982277529463	25.09929432285421	25.717595718750967	24.023287183100194
41	24.302115944104187	25.35585740970952	25.982635069609415	24.35939157657687
42	25.285067166510466	25.794998958658756	25.20468863896699	23.715245235863794
43	24.51516537014047	24.763135158178365	25.638514068705852	25.08318540297531
44	24.163510101010104	25.609184629803188	25.170194210142665	25.057111059044047
45	24.439060583084117	25.582742428334804	25.11039808393834	24.86779890464274
46	24.184993068617434	25.431340262546453	25.151807040722684	25.23185962811343
47	24.47615142551246	25.332825674519103	25.56306196366334	24.62796093630509
48	25.126588045402475	25.744884411121525	24.580372019160677	24.54815552431532
49	24.49113078792967	25.771154478211773	24.895622829882296	24.842091903976257
50	24.4345805645408	26.137998138590408	24.733479989846856	24.69394130702194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	74.5
2	33.0
3	119.0
4	205.0
5	201.5
6	198.0
7	186.5
8	175.0
9	257.5
10	340.0
11	480.0
12	620.0
13	1098.0
14	1576.0
15	2071.0
16	2566.0
17	2720.0
18	2874.0
19	2888.0
20	2902.0
21	3068.0
22	3234.0
23	3566.5
24	3899.0
25	4056.0
26	4213.0
27	5077.0
28	5941.0
29	6953.5
30	7966.0
31	9147.0
32	10328.0
33	12037.5
34	13747.0
35	15731.5
36	17716.0
37	20527.5
38	23339.0
39	25113.0
40	26887.0
41	29055.5
42	31224.0
43	31993.5
44	32763.0
45	35997.5
46	39232.0
47	41400.0
48	43568.0
49	45182.5
50	46797.0
51	45825.5
52	44854.0
53	44276.5
54	43699.0
55	43774.5
56	43850.0
57	42098.5
58	40347.0
59	36816.0
60	33285.0
61	29459.5
62	25634.0
63	22180.0
64	18726.0
65	15954.0
66	13182.0
67	11259.5
68	9337.0
69	8277.0
70	7217.0
71	5697.0
72	4177.0
73	3591.5
74	3006.0
75	2395.0
76	1784.0
77	1515.5
78	1247.0
79	999.5
80	752.0
81	628.0
82	504.0
83	402.5
84	301.0
85	205.5
86	110.0
87	83.0
88	56.0
89	36.0
90	16.0
91	15.5
92	15.0
93	12.0
94	9.0
95	11.0
96	13.0
97	9.5
98	6.0
99	10.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008623494956068989
2	1.6270745200130165E-4
3	1.6270745200130165E-4
4	0.0
5	0.0
6	0.0
7	1.6270745200130165E-4
8	0.0
9	6.508298080052066E-4
10	0.0013016596160104132
11	0.0
12	0.0
13	4.8812235600390497E-4
14	1.6270745200130165E-4
15	0.004230393752033843
16	6.508298080052066E-4
17	0.0
18	1.6270745200130165E-4
19	3.254149040026033E-4
20	8.135372600065082E-4
21	0.0
22	0.0
23	4.8812235600390497E-4
24	4.8812235600390497E-4
25	0.0017897819720143183
26	0.006020175724048161
27	0.005206638464041653
28	0.004067686300032541
29	0.0011389521640091118
30	0.0030914415880247317
31	0.006671005532053368
32	0.0026033192320208264
33	0.0056947608200455585
34	0.0022779043280182236
35	0.0037422713960299386
36	0.007647250244061178
37	0.0039049788480312398
38	0.007484542792059877
39	0.0039049788480312398
40	0.0021151968760169215
41	0.004555808656036447
42	0.0013016596160104132
43	0.001464367068011715
44	0.0013016596160104132
45	9.762447120078099E-4
46	6.508298080052066E-4
47	0.0022779043280182236
48	0.0013016596160104132
49	3.254149040026033E-4
50	0.0019524894240156199
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	614600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.59525019001158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14691070788913	50.16815927716839
2	12.528777301710194	15.68483899550976
3	3.4785305124098285	6.532184631536471
4	1.3629959326443575	3.412682856473669
5	0.6839177923915257	2.1405002662073978
6	0.40734461233769625	1.5298702753698827
7	0.27019736072839773	1.1839149976832406
8	0.17656282197416054	0.8841595212581642
9	0.13480424125179138	0.7594294687047506
>10	0.6828354458478767	7.791444333901504
>50	0.0736114516050914	3.2345584394574063
>100	0.05194561962328655	6.134970473757546
>500	0.0015661995866263292	0.5432864629718716
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	652	0.10608525870484868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6270745200130165E-4
2	0.0	0.0	0.0	0.0	1.6270745200130165E-4
3	0.0	0.0	0.0	0.0	1.6270745200130165E-4
4	0.0	0.0	0.0	1.6270745200130165E-4	1.6270745200130165E-4
5	0.0	0.0	0.0	1.6270745200130165E-4	1.6270745200130165E-4
6	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
7	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
8	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
9	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
10	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
11	0.0	0.0	0.0	1.6270745200130165E-4	6.508298080052066E-4
12	0.0	0.0	0.0	1.6270745200130165E-4	9.762447120078099E-4
13	0.0	0.0	0.0	1.6270745200130165E-4	0.0011389521640091116
14	0.0	0.0	0.0	1.6270745200130165E-4	0.0011389521640091116
15	0.0	0.0	0.0	1.6270745200130165E-4	0.0011389521640091116
16	0.0	0.0	0.0	1.6270745200130165E-4	0.0011389521640091116
17	0.0	0.0	0.0	1.6270745200130165E-4	0.0011389521640091116
18	0.0	0.0	0.0	1.6270745200130165E-4	0.0013016596160104132
19	0.0	0.0	0.0	1.6270745200130165E-4	0.0013016596160104132
20	0.0	0.0	0.0	4.8812235600390497E-4	0.0013016596160104132
21	0.0	0.0	0.0	6.508298080052066E-4	0.001464367068011715
22	0.0	0.0	0.0	9.762447120078099E-4	0.0016270745200130166
23	0.0	0.0	0.0	0.0026033192320208264	0.0017897819720143183
24	0.0	0.0	0.0	0.004230393752033843	0.0017897819720143183
25	0.0	0.0	0.0	0.005532053368044257	0.0019524894240156199
26	0.0	0.0	0.0	0.007159127888057273	0.0019524894240156199
27	0.0	0.0	0.0	0.010087862024080702	0.0021151968760169215
28	0.0	0.0	0.0	0.015457207940123658	0.0021151968760169215
29	0.0	0.0	0.0	0.024243410348193946	0.0021151968760169215
30	0.0	0.0	0.0	0.04490725675235926	0.0021151968760169215
31	0.0	0.0	0.0	0.07207940123657663	0.0021151968760169215
32	0.0	0.0	0.0	0.10218027985681744	0.0021151968760169215
33	0.0	0.0	0.0	0.13163032866905305	0.0021151968760169215
34	0.0	0.0	0.0	0.1657988935893264	0.002277904328018223
35	0.0	0.0	0.0	0.20826553856166613	0.002277904328018223
36	0.0	0.0	0.0	0.2660266840221282	0.002277904328018223
37	0.0	0.0	0.0	0.34575333550276605	0.002277904328018223
38	0.0	0.0	0.0	0.44956068987959646	0.002277904328018223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	105	1.1277734E-10	25.143602	7
CTATTAG	45	0.0013965291	24.447157	1
GTCCTAA	115	3.4924597E-10	22.95907	1
TCTAATC	80	3.60983E-6	22.000652	3
ACCGTCG	120	5.966285E-10	21.99886	8
TGGTCGG	145	1.2732926E-11	21.242008	37
ATACCGT	125	9.931682E-10	21.120625	6
GTATTAC	55	0.004478271	20.002218	1
GTTATAT	55	0.004478271	20.002218	1
CGTTTAT	145	2.9467628E-10	19.723116	31
GATATAC	105	1.7878647E-6	18.859236	1
CTAGTAC	70	8.115504E-4	18.857702	3
TCCTACA	175	9.094947E-12	18.8577	2
CGGCATC	165	8.185452E-11	18.66722	25
GACAGTC	95	1.5946223E-5	18.526865	7
TAGCGTA	85	1.4290598E-4	18.118183	7
CGTCGTA	135	5.5157216E-8	17.924997	10
TAGGACA	160	1.0968506E-9	17.87553	4
TCGTTTA	160	1.0986696E-9	17.874075	30
CATCGTT	185	2.1827873E-11	17.836914	28
>>END_MODULE