FastQCFastQC Report
Thu 2 Feb 2017
SRR4064229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177671
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53302.999926831052901No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40272.266548845900569No Hit
TATCAACGCAGAGTACTTTTTTTTT37212.0943204011909655No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26731.504466119963303No Hit
GTACATGGGGTGGTATCAACGCAAA23521.323795104434601No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21331.200533570475767No Hit
GGTATCAACGCAGAGTACTTTTTTT19761.112167995902539No Hit
GTATCAACGCAGAGTACATGGGGTG16650.9371253609199026No Hit
GTACATGGGTGGTATCAACGCAAAA11620.6540178194528089No Hit
TATCAACGCAGAGTACATGGGGTGG10300.5797231962447444No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9970.5611495404427285No Hit
GTACATGGGAGTGGTATCAACGCAA9170.5161224960742046No Hit
GTACATGGAAGCAGTGGTATCAACG8210.46209004283197597No Hit
GGTATCAACGCAGAGTACATGGGGT8130.45758733839512356No Hit
ATCAACGCAGAGTACTTTTTTTTTT7190.40468056126210805No Hit
TCCATGTACTCTGCGTTGATACCAC6940.3906096098969444No Hit
ACGCAGAGTACATGGGGTGGTATCA6330.35627648856594496No Hit
GTATCAACGCAGAGTACATGGGAGT6330.35627648856594496No Hit
CTGTAGGACGTGGAATATGGCAAGA6250.35177378412909255No Hit
GTATCAACGCAGAGTACATGGGAAG6150.3461454035830271No Hit
GTCCTAAAGTGTGTATTTCTCATTT5880.33094877610865026No Hit
GTCCTACAGTGGACATTTCTAAATT5230.29436430255922463No Hit
CTTTAGGACGTGAAATATGGCGAGG5210.29323862645001153No Hit
GTATCAACGCAGAGTACATGGGTGG4950.2786048370302413No Hit
GTACATGGGAAGCAGTGGTATCAAC4750.26734807593811033No Hit
CCCATGTACTCTGCGTTGATACCAC4240.23864333515317637No Hit
GATATACACTGTTCTACAAATCCCG3980.22400954573340615No Hit
GGTATCAACGCAGAGTACATGGGAA3890.2189440032419472No Hit
TTGTAGAACAGTGTATATCAATGAG3860.21725548907812758No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3840.21612981296891448No Hit
GAGTACATGGGGTGGTATCAACGCA3770.21218994658666865No Hit
CATGTACTCTGCGTTGATACCACTG3770.21218994658666865No Hit
TATCAACGCAGAGTACATGGGAAGC3620.2037473757675704No Hit
GAGTACATGGAAGCAGTGGTATCAA3510.19755615716689837No Hit
GTATCAACGCAGAGTACATGGGCAG3260.18348520580173466No Hit
TATCAACGCAGAGTACATGGGTGGT3180.17898250136488228No Hit
TATCAACGCAGAGTACATGGGAGTG3170.17841966331027573No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3130.17616831109184955No Hit
CAACGCAGAGTACTTTTTTTTTTTT3110.17504263498263645No Hit
GGTATCAACGCAGAGTACATGGGAG3060.1722284447096037No Hit
GGTGGTATCAACGCAAAAAAAAAAA3060.1722284447096037No Hit
ATCAACGCAGAGTACATGGGGTGGT3040.17110276860039061No Hit
GTGTATATCAATGAGTTACAATGAA2920.16434871194511202No Hit
ATTTAGAAATGTCCACTGTAGGACG2920.16434871194511202No Hit
GTCCTACAGTGTGCATTTCTCATTT2710.15252911279837453No Hit
GGTATCAACGCAGAGTACATGGGTG2670.15027776057994832No Hit
GTCCTTCAGTGTGCATTTCTCATTT2530.14239802781545666No Hit
ACGCAGAGTACATGGGAGTGGTATC2490.14014667559703048No Hit
GTATCAACGCAGAGTACATGGGGTA2490.14014667559703048No Hit
GTACATGGGGTATCAACGCAAAAAA2480.13958383754242393No Hit
CTGAAGGACCTGGAATATGGCGAGA2470.13902099948781738No Hit
GTACATGGGCAGTGGTATCAACGCA2400.13508113310557154No Hit
AACGCAGAGTACTTTTTTTTTTTTT2370.1333926189417519No Hit
TCAACGCAGAGTACTTTTTTTTTTT2330.1311412667233257No Hit
GTGTATATCAATGAGTTACAATGAG2320.13057842866871916No Hit
ACGCAGAGTACATGGGTGGTATCAA2280.12832707645029295No Hit
GATATACACTGTTCTACAATGCCGG2160.12157301979501438No Hit
GTTCTACAGTGTGGTTTTTATCATT2160.12157301979501438No Hit
TTTCTAAATTTTCCACCTTTTTCAG2080.11707031535816201No Hit
GAGTGGTATCAACGCAAAAAAAAAA2070.11650747730355546No Hit
GAATATGGCAAGAAAACTGAAAATC1970.11087909675748997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1950.10975342064827687No Hit
CATGGAAGCAGTGGTATCAACGCAG1950.10975342064827687No Hit
GTATCAACGCAGAGTACATGGGGGT1950.10975342064827687No Hit
CTGTAGGACCTGGAATATGGCGAGA1940.10919058259367032No Hit
GCGTTGATACCACTGCTTCCATGTA1900.10693923037524414No Hit
TATCAACGCAGAGTACATGGGGTAT1890.10637639232063759No Hit
GCGTTGATACCACTGCTTCCCATGT1840.10356220204760486No Hit
GAGTACATGGGAAGCAGTGGTATCA1780.10018517371996555No Hit
GCTTCCATGTACTCTGCGTTGATAC1780.10018517371996555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT350.002155531416.29078911
GGTTTCC551.1138149E-515.55029814
CGGTTTC508.621845E-515.20473713
GTCCTAA1250.015.187611
CCGGTTT456.7047845E-414.78238212
TCCAACG653.3111464E-614.62406218
TCCTAAA1300.014.59935952
TGCCGGT400.00524389214.2544410
ATTTCTA1550.014.10514315
TGAAGGA707.2581133E-613.5565482
TTAGAAA852.6987618E-713.3932883
GGACCTG1500.013.3003946
AGGACCT1500.013.2854175
CTGAAGG655.441111E-513.1431241
TAGAAAT801.9955678E-613.0481784
GAAGGAC802.0018942E-613.0445053
TATACAC1403.6379788E-1212.8750953
CACTGTT1207.1486284E-1012.6706137
AAATGTC751.4565079E-512.6706137
TCTAAAT1457.2759576E-1212.455667518
Produced by FastQC (version 0.11.5)