##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064229_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177671
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.597807182939253	32.0	32.0	32.0	32.0	32.0
2	30.638359664773656	32.0	32.0	32.0	32.0	32.0
3	30.76090639440314	32.0	32.0	32.0	32.0	32.0
4	30.65943794991867	32.0	32.0	32.0	32.0	32.0
5	30.8739693028125	32.0	32.0	32.0	32.0	32.0
6	34.42628791417845	36.0	36.0	36.0	32.0	36.0
7	34.086266188629544	36.0	36.0	36.0	32.0	36.0
8	33.90073788068959	36.0	36.0	36.0	32.0	36.0
9	33.7859245459304	36.0	36.0	36.0	32.0	36.0
10	33.920915624947234	36.0	36.0	36.0	32.0	36.0
11	34.228906236808484	36.0	36.0	36.0	32.0	36.0
12	34.03879642710403	36.0	36.0	36.0	32.0	36.0
13	34.07428899482752	36.0	36.0	36.0	32.0	36.0
14	34.07242600086677	36.0	36.0	36.0	32.0	36.0
15	34.15085185539564	36.0	36.0	36.0	32.0	36.0
16	34.14322539975573	36.0	36.0	36.0	32.0	36.0
17	34.06533986975927	36.0	36.0	36.0	32.0	36.0
18	34.084662100173915	36.0	36.0	36.0	32.0	36.0
19	33.956216827732156	36.0	36.0	36.0	32.0	36.0
20	33.70603531245955	36.0	36.0	36.0	27.0	36.0
21	33.580764446645766	36.0	36.0	36.0	27.0	36.0
22	33.672056779102945	36.0	36.0	36.0	27.0	36.0
23	33.73152624795268	36.0	36.0	36.0	27.0	36.0
24	33.73707020279055	36.0	36.0	36.0	27.0	36.0
25	33.21978826032386	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	8.0
7	3.0
8	14.0
9	6.0
10	20.0
11	7.0
12	13.0
13	14.0
14	104.0
15	146.0
16	219.0
17	257.0
18	373.0
19	415.0
20	572.0
21	712.0
22	801.0
23	1090.0
24	1394.0
25	1748.0
26	2226.0
27	2795.0
28	3730.0
29	4606.0
30	6068.0
31	8142.0
32	11496.0
33	16322.0
34	36126.0
35	78241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.59630651427285	18.569337312088283	13.455323461516807	18.379032712122065
2	12.054641856067002	23.094458140555872	45.73638175002533	19.114518253351793
3	19.457421061518545	33.89936398941858	28.92553610626442	17.717678842798446
4	9.777224936398225	17.343021815971362	37.26501114438165	35.61474210324876
5	9.301841229813174	43.47689034240908	31.873371122356502	15.347897305421244
6	25.032086645200515	38.802323748620836	21.37758663394204	14.788002972236608
7	23.750091480749635	35.728721577634786	22.293154988093427	18.22803195352215
8	28.14673010331916	32.957968525660874	20.60358661073731	18.291714760282648
9	30.403712192457355	13.381800571019895	22.152080505921376	34.062406730601374
10	17.639472409637197	29.16783996575844	31.204311733366374	21.98837589123799
11	31.04162912236439	24.762907731122723	25.87459452153541	18.320868624977475
12	27.163977359128154	27.284503393314747	31.850975753991722	13.700543493565373
13	30.747005052126454	23.912272105165336	29.105204760376008	16.235518082332202
14	23.146677480765547	23.267209624548006	32.063239949083616	21.52287294560283
15	21.130028780138886	31.477361689185763	31.75840453273107	15.634204997944275
16	16.51533756153612	29.52719981074474	34.541900888823804	19.415561738895335
17	15.529584049114309	28.795516629586864	32.7770424399448	22.897856881354024
18	16.12407118432097	27.412949056678176	39.845418030431915	16.617561728568933
19	20.33742676881478	25.5312077512393	36.074808472284815	18.05655700766111
20	23.26291476536533	24.497211424708468	36.37766886372599	15.862204946200215
21	24.179092706614014	23.51042435372387	32.861641420258344	19.448841519403775
22	22.05873240309379	26.958544815426126	33.780426663361816	17.202296118118266
23	19.19124771841269	28.555332717398652	35.41248845122473	16.840931112963922
24	19.66662723426788	28.476388442927238	36.14655332668616	15.710430996118726
25	19.606815941416702	28.5385157020138	36.18250950570342	15.672158850866074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	355.0
1	355.0
2	676.0
3	997.0
4	997.0
5	997.0
6	2767.5
7	4538.0
8	4538.0
9	4538.0
10	4011.0
11	3484.0
12	3484.0
13	3484.0
14	2227.0
15	970.0
16	970.0
17	970.0
18	1906.0
19	2842.0
20	2842.0
21	2842.0
22	4644.5
23	6447.0
24	6447.0
25	6447.0
26	10076.0
27	13705.0
28	13705.0
29	13705.0
30	16805.5
31	19906.0
32	19906.0
33	19906.0
34	18531.5
35	17157.0
36	17157.0
37	17157.0
38	16319.5
39	15482.0
40	15482.0
41	15482.0
42	16758.0
43	18034.0
44	18034.0
45	18034.0
46	22749.0
47	27464.0
48	27464.0
49	27464.0
50	25138.0
51	22812.0
52	22812.0
53	22812.0
54	15590.0
55	8368.0
56	8368.0
57	8368.0
58	6939.0
59	5510.0
60	5510.0
61	5510.0
62	4617.0
63	3724.0
64	3724.0
65	3724.0
66	3216.5
67	2709.0
68	2709.0
69	2709.0
70	2155.0
71	1601.0
72	1601.0
73	1601.0
74	1251.5
75	902.0
76	902.0
77	902.0
78	636.0
79	370.0
80	370.0
81	370.0
82	242.5
83	115.0
84	115.0
85	115.0
86	78.0
87	41.0
88	41.0
89	41.0
90	33.0
91	25.0
92	25.0
93	25.0
94	21.0
95	17.0
96	17.0
97	17.0
98	56.5
99	96.0
100	96.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03433312133099943
2	0.0028141902730327403
3	5.628380546065481E-4
4	0.0016885141638196444
5	0.010131084982917864
6	0.015196627474376797
7	0.021387846075048827
8	0.037147311604032174
9	0.05290677713301552
10	0.06134934795211374
11	0.05797231962447445
12	0.06528921433435958
13	0.06866624266199886
14	0.07035475682581852
15	0.06641489044357267
16	0.07485746126267089
17	0.07091759488042505
18	0.09005408873704769
19	0.08498854624558876
20	0.09061692679165423
21	0.0866770604094084
22	0.0866770604094084
23	0.09174260290086733
24	0.08555138430019531
25	0.08217435597255601
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	177671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.460485954376345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82991286129233	25.471236161219334
2	14.724695649413519	10.442897265169893
3	5.196577940732981	5.5281953723455155
4	2.479247019983175	3.516612165181712
5	1.4078694665333398	2.4961867721800406
6	0.8586892687649795	1.826972325252855
7	0.5555291017888037	1.3789532337860426
8	0.40474263130327126	1.148189631397358
9	0.3047473929812866	0.972584158360115
>10	1.8824500420614891	12.388628419944729
>50	0.16507150453153024	4.017538033781539
>100	0.15396092249575416	11.018117756977784
>500	0.02063393806644128	5.121263458864981
>1k	0.014285034045997808	11.6726984144852
>5k	0.0015872260051108679	2.999926831052901
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5330	2.999926831052901	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4027	2.266548845900569	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3721	2.0943204011909655	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2673	1.504466119963303	No Hit
GTACATGGGGTGGTATCAACGCAAA	2352	1.323795104434601	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2133	1.200533570475767	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1976	1.112167995902539	No Hit
GTATCAACGCAGAGTACATGGGGTG	1665	0.9371253609199026	No Hit
GTACATGGGTGGTATCAACGCAAAA	1162	0.6540178194528089	No Hit
TATCAACGCAGAGTACATGGGGTGG	1030	0.5797231962447444	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	997	0.5611495404427285	No Hit
GTACATGGGAGTGGTATCAACGCAA	917	0.5161224960742046	No Hit
GTACATGGAAGCAGTGGTATCAACG	821	0.46209004283197597	No Hit
GGTATCAACGCAGAGTACATGGGGT	813	0.45758733839512356	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	719	0.40468056126210805	No Hit
TCCATGTACTCTGCGTTGATACCAC	694	0.3906096098969444	No Hit
ACGCAGAGTACATGGGGTGGTATCA	633	0.35627648856594496	No Hit
GTATCAACGCAGAGTACATGGGAGT	633	0.35627648856594496	No Hit
CTGTAGGACGTGGAATATGGCAAGA	625	0.35177378412909255	No Hit
GTATCAACGCAGAGTACATGGGAAG	615	0.3461454035830271	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	588	0.33094877610865026	No Hit
GTCCTACAGTGGACATTTCTAAATT	523	0.29436430255922463	No Hit
CTTTAGGACGTGAAATATGGCGAGG	521	0.29323862645001153	No Hit
GTATCAACGCAGAGTACATGGGTGG	495	0.2786048370302413	No Hit
GTACATGGGAAGCAGTGGTATCAAC	475	0.26734807593811033	No Hit
CCCATGTACTCTGCGTTGATACCAC	424	0.23864333515317637	No Hit
GATATACACTGTTCTACAAATCCCG	398	0.22400954573340615	No Hit
GGTATCAACGCAGAGTACATGGGAA	389	0.2189440032419472	No Hit
TTGTAGAACAGTGTATATCAATGAG	386	0.21725548907812758	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	384	0.21612981296891448	No Hit
GAGTACATGGGGTGGTATCAACGCA	377	0.21218994658666865	No Hit
CATGTACTCTGCGTTGATACCACTG	377	0.21218994658666865	No Hit
TATCAACGCAGAGTACATGGGAAGC	362	0.2037473757675704	No Hit
GAGTACATGGAAGCAGTGGTATCAA	351	0.19755615716689837	No Hit
GTATCAACGCAGAGTACATGGGCAG	326	0.18348520580173466	No Hit
TATCAACGCAGAGTACATGGGTGGT	318	0.17898250136488228	No Hit
TATCAACGCAGAGTACATGGGAGTG	317	0.17841966331027573	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	313	0.17616831109184955	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	311	0.17504263498263645	No Hit
GGTATCAACGCAGAGTACATGGGAG	306	0.1722284447096037	No Hit
GGTGGTATCAACGCAAAAAAAAAAA	306	0.1722284447096037	No Hit
ATCAACGCAGAGTACATGGGGTGGT	304	0.17110276860039061	No Hit
GTGTATATCAATGAGTTACAATGAA	292	0.16434871194511202	No Hit
ATTTAGAAATGTCCACTGTAGGACG	292	0.16434871194511202	No Hit
GTCCTACAGTGTGCATTTCTCATTT	271	0.15252911279837453	No Hit
GGTATCAACGCAGAGTACATGGGTG	267	0.15027776057994832	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	253	0.14239802781545666	No Hit
ACGCAGAGTACATGGGAGTGGTATC	249	0.14014667559703048	No Hit
GTATCAACGCAGAGTACATGGGGTA	249	0.14014667559703048	No Hit
GTACATGGGGTATCAACGCAAAAAA	248	0.13958383754242393	No Hit
CTGAAGGACCTGGAATATGGCGAGA	247	0.13902099948781738	No Hit
GTACATGGGCAGTGGTATCAACGCA	240	0.13508113310557154	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	237	0.1333926189417519	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	233	0.1311412667233257	No Hit
GTGTATATCAATGAGTTACAATGAG	232	0.13057842866871916	No Hit
ACGCAGAGTACATGGGTGGTATCAA	228	0.12832707645029295	No Hit
GATATACACTGTTCTACAATGCCGG	216	0.12157301979501438	No Hit
GTTCTACAGTGTGGTTTTTATCATT	216	0.12157301979501438	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	208	0.11707031535816201	No Hit
GAGTGGTATCAACGCAAAAAAAAAA	207	0.11650747730355546	No Hit
GAATATGGCAAGAAAACTGAAAATC	197	0.11087909675748997	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	195	0.10975342064827687	No Hit
CATGGAAGCAGTGGTATCAACGCAG	195	0.10975342064827687	No Hit
GTATCAACGCAGAGTACATGGGGGT	195	0.10975342064827687	No Hit
CTGTAGGACCTGGAATATGGCGAGA	194	0.10919058259367032	No Hit
GCGTTGATACCACTGCTTCCATGTA	190	0.10693923037524414	No Hit
TATCAACGCAGAGTACATGGGGTAT	189	0.10637639232063759	No Hit
GCGTTGATACCACTGCTTCCCATGT	184	0.10356220204760486	No Hit
GAGTACATGGGAAGCAGTGGTATCA	178	0.10018517371996555	No Hit
GCTTCCATGTACTCTGCGTTGATAC	178	0.10018517371996555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGTT	35	0.0021555314	16.290789	11
GGTTTCC	55	1.1138149E-5	15.550298	14
CGGTTTC	50	8.621845E-5	15.204737	13
GTCCTAA	125	0.0	15.18761	1
CCGGTTT	45	6.7047845E-4	14.782382	12
TCCAACG	65	3.3111464E-6	14.624062	18
TCCTAAA	130	0.0	14.5993595	2
TGCCGGT	40	0.005243892	14.25444	10
ATTTCTA	155	0.0	14.105143	15
TGAAGGA	70	7.2581133E-6	13.556548	2
TTAGAAA	85	2.6987618E-7	13.393288	3
GGACCTG	150	0.0	13.300394	6
AGGACCT	150	0.0	13.285417	5
CTGAAGG	65	5.441111E-5	13.143124	1
TAGAAAT	80	1.9955678E-6	13.048178	4
GAAGGAC	80	2.0018942E-6	13.044505	3
TATACAC	140	3.6379788E-12	12.875095	3
CACTGTT	120	7.1486284E-10	12.670613	7
AAATGTC	75	1.4565079E-5	12.670613	7
TCTAAAT	145	7.2759576E-12	12.4556675	18
>>END_MODULE