##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177671
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.240928457654878	32.0	32.0	32.0	21.0	32.0
2	31.34907216146698	32.0	32.0	32.0	32.0	32.0
3	31.43588993139004	32.0	32.0	32.0	32.0	32.0
4	31.53313709046496	32.0	32.0	32.0	32.0	32.0
5	31.352770007485745	32.0	32.0	32.0	32.0	32.0
6	34.87659212814697	36.0	36.0	36.0	36.0	36.0
7	34.95776463238232	36.0	36.0	36.0	36.0	36.0
8	34.994472930303765	36.0	36.0	36.0	36.0	36.0
9	35.07028158787872	36.0	36.0	36.0	36.0	36.0
10	34.906236808483094	36.0	36.0	36.0	32.0	36.0
11	35.054730372429944	36.0	36.0	36.0	36.0	36.0
12	34.98668888000856	36.0	36.0	36.0	36.0	36.0
13	35.00656832009726	36.0	36.0	36.0	36.0	36.0
14	34.94448728267415	36.0	36.0	36.0	32.0	36.0
15	34.92909929026121	36.0	36.0	36.0	36.0	36.0
16	34.912619392022336	36.0	36.0	36.0	32.0	36.0
17	34.866641151341526	36.0	36.0	36.0	32.0	36.0
18	34.8255483449747	36.0	36.0	36.0	32.0	36.0
19	34.78975747308227	36.0	36.0	36.0	32.0	36.0
20	34.7350721276967	36.0	36.0	36.0	32.0	36.0
21	34.71885676334348	36.0	36.0	36.0	32.0	36.0
22	34.730451227268375	36.0	36.0	36.0	32.0	36.0
23	34.72220002138785	36.0	36.0	36.0	32.0	36.0
24	34.719149439131876	36.0	36.0	36.0	32.0	36.0
25	34.67213557643059	36.0	36.0	36.0	32.0	36.0
26	34.63659235328219	36.0	36.0	36.0	32.0	36.0
27	34.62357390907914	36.0	36.0	36.0	32.0	36.0
28	34.56700868459118	36.0	36.0	36.0	32.0	36.0
29	34.48113648259986	36.0	36.0	36.0	32.0	36.0
30	34.3941104625966	36.0	36.0	36.0	32.0	36.0
31	34.32938971469739	36.0	36.0	36.0	32.0	36.0
32	34.19604212280001	36.0	36.0	36.0	32.0	36.0
33	34.03680398039072	36.0	36.0	36.0	32.0	36.0
34	33.859284857967815	36.0	36.0	36.0	32.0	36.0
35	33.718288296908334	36.0	36.0	36.0	27.0	36.0
36	33.620264421318055	36.0	36.0	36.0	27.0	36.0
37	33.502214767744874	36.0	36.0	36.0	21.0	36.0
38	33.35730648220588	36.0	36.0	36.0	21.0	36.0
39	33.138064174794984	36.0	36.0	36.0	14.0	36.0
40	33.020791237737164	36.0	36.0	36.0	14.0	36.0
41	32.89573987876468	36.0	36.0	36.0	14.0	36.0
42	32.72165406847488	36.0	36.0	36.0	14.0	36.0
43	32.597154290795906	36.0	36.0	36.0	14.0	36.0
44	32.36824242560688	36.0	36.0	36.0	14.0	36.0
45	32.035126722988	36.0	36.0	36.0	14.0	36.0
46	31.764930686493575	36.0	36.0	36.0	14.0	36.0
47	31.42842669878596	36.0	32.0	36.0	14.0	36.0
48	31.16051578479324	36.0	32.0	36.0	14.0	36.0
49	30.97433458470994	36.0	32.0	36.0	14.0	36.0
50	30.381097646774094	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	6.0
22	28.0
23	106.0
24	351.0
25	822.0
26	1585.0
27	3114.0
28	4979.0
29	6818.0
30	8559.0
31	11045.0
32	14600.0
33	19560.0
34	31370.0
35	74725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.23341739091278	19.900256675822938	14.02710856937002	18.839217363894267
2	11.80228402253629	23.830831490017953	44.395476982478655	19.9714075049671
3	19.463049473743457	33.75415095401587	29.29250858332864	17.490290988912026
4	9.578378013294236	17.772174412256362	37.056131839185916	35.593315735263495
5	9.213658953909192	43.76347293593214	31.61742771752284	15.405440392635828
6	25.629956492618376	38.66753718952446	20.693866753718954	15.00863956413821
7	23.329637363441417	35.705883346184805	22.52815597368169	18.43632331669209
8	27.901570881010407	33.41794665420919	20.68429850679064	17.996183957989768
9	29.448812693123806	13.183918591103783	22.579937074705494	34.78733164106691
10	17.557733113451267	29.55012354295299	31.328691795509677	21.563451548086068
11	30.981595092024538	24.39691563010075	26.01058141498283	18.610907862891878
12	26.349263526405547	27.416404477939558	32.3530570548936	13.881274940761296
13	30.63938762874993	23.4665390893229	29.748409973546465	16.145663308380705
14	22.915388555251	23.190616363953602	32.359248273494266	21.534746807301136
15	21.480676790759983	30.952595377739755	31.855433350969818	15.711294480530446
16	16.71572738375987	28.79704622588942	34.87851140591317	19.60871498443753
17	15.627198586150806	28.54151774909805	33.35716014431168	22.474123520439466
18	16.353259676593254	26.310427700637696	40.48888113423125	16.847431488537804
19	20.68317283068143	24.60840542350749	36.84844459703609	17.859977148774984
20	23.060167726684302	24.191478583891485	37.15877750886475	15.589576180559463
21	23.687039528116575	22.999251425387374	34.21830236785969	19.095406678636355
22	21.892148971976294	26.45170005234394	34.77326068970175	16.882890285978018
23	18.850327577278968	27.605984195240563	36.51529819663642	17.028390030844047
24	19.7094629962121	27.637599833399936	36.9092311069336	15.743706063454363
25	19.787691511037565	27.54832100683305	36.81571037790011	15.848277104229282
26	16.1500576928489	29.417161511834074	38.412180226831396	16.020600568485634
27	16.65146909827761	29.37915118766183	38.00292693909715	15.966452774963413
28	15.629977767146034	30.12973855289449	36.810851884164016	17.42943179579546
29	15.870969920749278	28.139634365994237	36.56114913544668	19.428246577809798
30	15.858765521045582	29.777892852720335	36.396640812329025	17.966700813905057
31	17.16595179393653	29.53775316063809	35.21451810823286	18.08177693719252
32	15.734492055361999	31.322642921870692	35.70331012512172	17.239554897645597
33	16.167688263735275	30.92765516736743	36.17072772608815	16.73392884280914
34	16.774829032167283	30.976275574817773	36.15624912053584	16.092646272479104
35	16.663570917973917	32.205917945773734	34.839555788205764	16.29095534804658
36	16.300315784140995	32.83873618796193	34.43792111588321	16.423026912013867
37	16.33167667602875	33.57348297657978	33.2284554813218	16.86638486606967
38	17.0411689876054	34.710511206925666	32.39313737630729	15.85518242916165
39	17.453732889048993	32.57609870317003	33.30162554034582	16.668542867435157
40	18.710636070445606	33.604624329511616	32.666362730695845	15.01837686934693
41	16.999791738198027	32.823185730126475	34.234300155914916	15.94272237576058
42	17.196215391715963	36.05678038127508	32.031834837082855	14.715169389926096
43	15.360759173059712	35.67460473807741	32.1708589664935	16.793777122369377
44	15.241997669797994	35.56710024934287	31.52132922827537	17.669572852583766
45	15.981223722498324	35.61357790522933	30.435467802849086	17.96973056942326
46	16.85728437310039	33.8535920931175	29.658126393047706	19.630997140734404
47	18.985731573466918	34.5059522134354	28.667998761714465	17.84031745138322
48	17.11056577436567	35.795979017043024	27.224936398225903	19.8685188103654
49	18.16176056734395	35.08301908031744	28.381831485337987	18.37338886700062
50	16.711226184110544	37.28477753074607	26.7497818928883	19.254214392255086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	82.0
2	48.0
3	140.0
4	232.0
5	257.5
6	283.0
7	300.0
8	317.0
9	449.5
10	582.0
11	888.0
12	1194.0
13	2018.5
14	2843.0
15	4000.0
16	5157.0
17	5822.0
18	6487.0
19	6570.0
20	6653.0
21	6610.5
22	6568.0
23	6600.5
24	6633.0
25	5407.0
26	4181.0
27	4504.5
28	4828.0
29	5594.5
30	6361.0
31	7535.0
32	8709.0
33	10493.5
34	12278.0
35	13475.5
36	14673.0
37	13885.5
38	13098.0
39	11696.5
40	10295.0
41	9301.5
42	8308.0
43	7805.0
44	7302.0
45	7686.0
46	8070.0
47	8224.5
48	8379.0
49	7645.0
50	6911.0
51	6021.5
52	5132.0
53	4500.0
54	3868.0
55	3665.5
56	3463.0
57	3249.5
58	3036.0
59	2855.0
60	2674.0
61	2492.0
62	2310.0
63	2022.5
64	1735.0
65	1671.5
66	1608.0
67	1403.0
68	1198.0
69	1029.5
70	861.0
71	687.0
72	513.0
73	422.0
74	331.0
75	271.5
76	212.0
77	162.0
78	112.0
79	81.0
80	50.0
81	42.0
82	34.0
83	21.5
84	9.0
85	8.0
86	7.0
87	6.0
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008442570819098221
2	0.0011256761092130962
3	5.628380546065481E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	5.628380546065481E-4
12	0.0
13	5.628380546065481E-4
14	0.0
15	0.005065542491458932
16	0.0
17	0.0
18	0.0
19	0.0
20	5.628380546065481E-4
21	0.0
22	0.0
23	0.0016885141638196444
24	0.0
25	0.0028141902730327403
26	0.0033770283276392888
27	0.006191218600672028
28	0.0033770283276392888
29	0.003939866382245837
30	0.005065542491458932
31	0.009568246928311316
32	0.0022513522184261924
33	0.004502704436852385
34	0.0033770283276392888
35	0.004502704436852385
36	0.010131084982917864
37	0.0022513522184261924
38	0.0073168947098851255
39	0.003939866382245837
40	0.0011256761092130962
41	0.005628380546065481
42	0.0022513522184261924
43	0.0022513522184261924
44	0.0022513522184261924
45	0.0
46	0.0016885141638196444
47	0.0033770283276392888
48	0.0016885141638196444
49	5.628380546065481E-4
50	0.0033770283276392888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	177671.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.473391830968474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97168293467767	40.9143867035138
2	12.08194787085702	12.679615694176313
3	4.060924595087418	6.392714624221172
4	1.8481175587257321	3.8790798723483295
5	1.0640351818084308	2.7916767508484783
6	0.6607315241874934	2.0802494498258017
7	0.40330365762093745	1.4813897597244345
8	0.2831706532232114	1.1887139713290296
9	0.24670170545961603	1.1650747730355544
>10	1.2056205084200364	11.633299750662742
>50	0.0922449855196825	3.2368816520422583
>100	0.06972004719510887	7.057426366711505
>500	0.00858092888555186	3.1293795836124074
>1k	0.003217848332081948	2.370111047948174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1815	1.0215510691108847	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1221	0.6872252646745952	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1175	0.661334714162694	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	950	0.5346961518762207	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	799	0.4497076056306319	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	796	0.4480190914668123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.4176258365180587	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	724	0.4074947515351408	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	522	0.2938014645046181	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	514	0.2892987600677657	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	513	0.2887359220131592	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	402	0.22626089795183235	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	401	0.22569805989722577	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	384	0.21612981296891448	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	352	0.1981189952215049	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	347	0.1953048049484722	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	333	0.1874250721839805	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	318	0.17898250136488228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	311	0.17504263498263645	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	306	0.1722284447096037	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	302	0.1699770924911775	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	291	0.16378587389050547	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	254	0.1429608658700632	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	254	0.1429608658700632	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	249	0.14014667559703048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	245	0.13789532337860427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	242	0.13620680921478465	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	235	0.13226694283253881	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	232	0.13057842866871916	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	226	0.12720140034107988	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	215	0.12101018174040784	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	214	0.12044734368580129	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	212	0.11932166757658819	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	203	0.11425612508512925	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	201	0.11313044897591618	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	199	0.11200477286670307	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	189	0.10637639232063759	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	186	0.10468787815681793	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA	183	0.1029993639929983	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	182	0.10243652593839175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	181	0.1018736878837852	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	181	0.1018736878837852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.628380546065481E-4	0.0	0.0	0.0	0.0
7	5.628380546065481E-4	0.0	0.0	0.0	0.0
8	5.628380546065481E-4	0.0	0.0	0.0	0.0
9	5.628380546065481E-4	0.0	0.0	0.0	0.0
10	5.628380546065481E-4	0.0	0.0	0.0	0.0
11	5.628380546065481E-4	0.0	0.0	0.0	0.0
12	5.628380546065481E-4	0.0	0.0	0.0	0.0
13	5.628380546065481E-4	0.0	0.0	0.0	0.0
14	5.628380546065481E-4	0.0	0.0	0.0	0.0
15	0.0011256761092130962	0.0	0.0	0.0	0.0
16	0.0011256761092130962	0.0	0.0	5.628380546065481E-4	0.0
17	0.0011256761092130962	0.0	0.0	5.628380546065481E-4	0.0
18	0.0011256761092130962	0.0	0.0	0.0011256761092130962	0.0
19	0.0011256761092130962	0.0	0.0	0.0016885141638196442	0.0
20	0.0011256761092130962	0.0	0.0	0.0016885141638196442	0.0
21	0.0011256761092130962	0.0	0.0	0.004502704436852385	0.0
22	0.0011256761092130962	0.0	0.0	0.010131084982917865	0.0
23	0.0011256761092130962	0.0	0.0	0.025327712457294663	0.0
24	0.0011256761092130962	0.0	0.0	0.037147311604032174	0.0
25	0.0011256761092130962	0.0	0.0	0.04727839658695004	0.0
26	0.0011256761092130962	0.0	0.0	0.05797231962447445	0.0
27	0.0011256761092130962	0.0	0.0	0.0726061090442447	0.0
28	0.0011256761092130962	0.0	0.0	0.10693923037524414	0.0
29	0.0011256761092130962	0.0	0.0	0.1474635703069156	0.0
30	0.0011256761092130962	0.0	0.0	0.24314603959002876	0.0
31	0.0011256761092130962	0.0	0.0	0.37879001075020685	0.0
32	0.0011256761092130962	0.0	0.0	0.5268164191117289	0.0
33	0.0011256761092130962	0.0	0.0	0.6247502406132683	0.0
34	0.0011256761092130962	0.0	0.0	0.7311266329339059	0.0
35	0.0011256761092130962	0.0	0.0	0.884218583786887	0.0
36	0.0011256761092130962	0.0	0.0	1.0603868948787365	0.0
37	0.0011256761092130962	0.0	0.0	1.282707926448323	0.0
38	0.0011256761092130962	0.0	0.0	1.526979642147565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGGGA	20	7.838747E-4	43.999718	36
ATACTTG	35	7.255825E-6	37.714043	6
CTTAGTG	30	1.2967197E-4	36.666428	35
CTAATAC	25	0.0023474847	35.199776	3
ACGTTTC	35	3.2070297E-4	31.42837	29
CGTTTCT	50	2.346631E-6	30.799805	30
CAGTAGG	30	0.0057242964	29.341402	1
GGTAAAC	30	0.0057321806	29.333143	35
GTAGGAT	30	0.0057321806	29.333143	3
AGGCGGG	30	0.0057321806	29.333143	42
ACAGGCG	30	0.0057321806	29.333143	40
TCCGTAA	30	0.0057321806	29.333143	26
TCGAGTT	30	0.0057321806	29.333143	33
GTGTAGG	40	6.997118E-4	27.507565	1
TTCGTCA	170	0.0	27.176298	44
CTGTAGG	285	0.0	26.252836	1
TGTAGGA	315	0.0	25.841105	2
GATATAC	210	0.0	25.149775	1
GTCCTAA	240	0.0	24.75681	1
ACTGTTC	205	0.0	24.682768	8
>>END_MODULE