FastQCFastQC Report
Thu 2 Feb 2017
SRR4064228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280818
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37300.2912201421279214No Hit
TATCAACGCAGAGTACTTTTTTTTT26390.2060402024331326No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18770.14654697232549824No Hit
GCGCAAGACGGACCAGAGCGAAAGC15770.12312444078705952No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15200.11867415979475615No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14420.11258430159476208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13760.10743134465630558No Hit
GATTAAGAGGGACGGCCGGGGGCAT13520.10555754213323047No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13390.1045425657665648No Hit
GGTATCAACGCAGAGTACTTTTTTT13310.10391796492553979No Hit
GAATAACGCCGCCGCATCGCCAGTC13270.10360566450502726No Hit
GAATAGGACCGCGGTTCTATTTTGT13200.10305913876913035No Hit
GTATCTGATCGTCTTCGAACCTCCG13110.10235646282297718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC504.583864E-617.091043
CCGAACG350.002167892916.287999
GCGAAAT653.3674787E-614.61685812
GCGTTAT759.709074E-713.9282071
CCTACGA707.2936855E-613.5653762
AAATCGC852.6905946E-713.41468814
GCTCGCG500.001498157213.3013413
TATGCCG500.001503458813.2951065
TAGGCCC905.436923E-712.6600284
ACGGTAT2450.012.4098989
CGAAATT853.937341E-612.29535713
CGCGCTA701.0879966E-412.21742516
GTGTAGG1405.2750693E-1112.2097921
CGTTGTA550.003078205312.0855172
CGCGGTC1501.4551915E-1112.03501710
CGCGTAA951.0375315E-612.00167810
AAGACGG3200.011.8706315
ACGCGCG658.0149464E-411.69348613
TAAGACA658.0570794E-411.686184
AACCGCG1552.5465852E-1111.6458797
Produced by FastQC (version 0.11.5)