##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064228_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1280818
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21269844739846	32.0	32.0	32.0	32.0	32.0
2	30.898371197156816	32.0	32.0	32.0	32.0	32.0
3	30.92281885482559	32.0	32.0	32.0	32.0	32.0
4	30.94535523392082	32.0	32.0	32.0	32.0	32.0
5	30.88365716284437	32.0	32.0	32.0	32.0	32.0
6	34.484725386432736	36.0	36.0	36.0	32.0	36.0
7	34.42623542142599	36.0	36.0	36.0	32.0	36.0
8	34.38808714430934	36.0	36.0	36.0	32.0	36.0
9	34.498509546243106	36.0	36.0	36.0	32.0	36.0
10	34.26578483437928	36.0	36.0	36.0	32.0	36.0
11	34.463718498646955	36.0	36.0	36.0	32.0	36.0
12	34.33408649784747	36.0	36.0	36.0	32.0	36.0
13	34.396103896103895	36.0	36.0	36.0	32.0	36.0
14	34.31715591130043	36.0	36.0	36.0	32.0	36.0
15	34.266634291523076	36.0	36.0	36.0	32.0	36.0
16	34.2601275122617	36.0	36.0	36.0	32.0	36.0
17	34.18739508657748	36.0	36.0	36.0	32.0	36.0
18	34.188552940386536	36.0	36.0	36.0	32.0	36.0
19	34.191781345983586	36.0	36.0	36.0	32.0	36.0
20	34.17451894023975	36.0	36.0	36.0	32.0	36.0
21	34.14542737531796	36.0	36.0	36.0	32.0	36.0
22	34.13804147037284	36.0	36.0	36.0	32.0	36.0
23	34.08820300776535	36.0	36.0	36.0	32.0	36.0
24	34.07167138500552	36.0	36.0	36.0	32.0	36.0
25	33.677456125694675	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	14.0
5	15.0
6	53.0
7	22.0
8	68.0
9	77.0
10	126.0
11	51.0
12	86.0
13	66.0
14	119.0
15	229.0
16	384.0
17	495.0
18	598.0
19	813.0
20	1189.0
21	1727.0
22	2633.0
23	3999.0
24	6009.0
25	8801.0
26	12952.0
27	17377.0
28	24304.0
29	33610.0
30	45206.0
31	62608.0
32	89657.0
33	127499.0
34	272364.0
35	567661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9956558651678	17.116297378757476	11.011858503983687	25.876188252091037
2	16.964707480677383	19.393513034909933	37.33319383206598	26.308585652346707
3	18.95051038652586	23.451234156312413	28.541670245176036	29.05658521198569
4	12.409078274665593	14.725181374983212	36.13008404306153	36.735656307289666
5	14.526109814498	36.47995177588278	33.51989986624366	15.47403854337555
6	34.32296563291594	35.89084866676453	16.372153385328005	13.41403231499152
7	30.358353299118175	30.42371716578526	20.576966929163373	18.640962605933193
8	28.397827165122173	32.88790999371268	19.12461778178708	19.589645059378064
9	27.520826742592007	13.544315168273831	18.57358782382509	40.361270265309074
10	15.83946640876632	26.406844566867253	31.41410582611569	26.339583198250736
11	37.726262503954786	20.916338240521213	22.283502396306524	19.07389685921748
12	24.453612854385042	23.564159034499248	28.98158514186339	23.00064296925232
13	29.3042888593406	19.35478830700289	25.598157520152125	25.742765313504385
14	23.343195474300753	19.512898258487052	25.183850672550097	31.9600555946621
15	25.262396398831587	27.221023898677295	22.207387488877206	25.309192213613912
16	25.64674687085688	25.803545828343033	23.785862780990062	24.76384451981003
17	23.690197359066627	25.877772065981148	25.269726141052907	25.162304433899322
18	24.458138778980004	24.959917115157428	26.54885580162644	24.033088304236127
19	25.615933281610015	24.496068793573183	25.528659952011388	24.359337972805413
20	25.540677699911473	24.174713965693854	25.221103756830015	25.06350457756466
21	27.07163456317082	23.710045778051068	24.440588153024798	24.777731505753316
22	25.69341023914039	24.056401064513054	25.236241575079266	25.01394712126729
23	24.21818960357435	24.171150489222416	25.96574739839317	25.64491250881007
24	24.673970029698395	24.814375243677663	25.48366353691438	25.027991189709553
25	24.515490853629952	24.505177161109255	25.631010460584502	25.348321524676294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	78.0
2	257.0
3	436.0
4	436.0
5	436.0
6	1496.0
7	2556.0
8	2556.0
9	2556.0
10	2635.0
11	2714.0
12	2714.0
13	2714.0
14	3027.0
15	3340.0
16	3340.0
17	3340.0
18	5517.0
19	7694.0
20	7694.0
21	7694.0
22	12402.5
23	17111.0
24	17111.0
25	17111.0
26	25762.5
27	34414.0
28	34414.0
29	34414.0
30	43841.0
31	53268.0
32	53268.0
33	53268.0
34	65727.5
35	78187.0
36	78187.0
37	78187.0
38	90340.0
39	102493.0
40	102493.0
41	102493.0
42	119897.5
43	137302.0
44	137302.0
45	137302.0
46	152743.5
47	168185.0
48	168185.0
49	168185.0
50	172807.0
51	177429.0
52	177429.0
53	177429.0
54	163039.0
55	148649.0
56	148649.0
57	148649.0
58	136760.0
59	124871.0
60	124871.0
61	124871.0
62	110375.5
63	95880.0
64	95880.0
65	95880.0
66	78786.5
67	61693.0
68	61693.0
69	61693.0
70	46228.0
71	30763.0
72	30763.0
73	30763.0
74	23551.5
75	16340.0
76	16340.0
77	16340.0
78	13223.0
79	10106.0
80	10106.0
81	10106.0
82	6859.0
83	3612.0
84	3612.0
85	3612.0
86	2769.0
87	1926.0
88	1926.0
89	1926.0
90	1360.5
91	795.0
92	795.0
93	795.0
94	551.5
95	308.0
96	308.0
97	308.0
98	488.0
99	668.0
100	668.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03677337451534879
2	0.005074881833328389
3	0.0018738025230750973
4	0.0035914548358939365
5	0.010227838771784906
6	0.013585068292294456
7	0.02295408090766994
8	0.03614877367432375
9	0.04786003944354311
10	0.05754135247943112
11	0.05644830100763731
12	0.06394351109993769
13	0.06277238452301576
14	0.06550501320250028
15	0.06160125794609382
16	0.06519271278198775
17	0.06370928578455332
18	0.07573285197428518
19	0.07268792287428814
20	0.07721627897171963
21	0.07393712455633822
22	0.076825903446079
23	0.08057350849222919
24	0.07424942497685073
25	0.07542055155377267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1280818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.477305968808466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89971756947689	33.60760066915631
2	13.804944014268441	12.556233256383134
3	4.90545351059418	6.692604306511714
4	2.1886983697410187	3.981444217365784
5	1.1922026944802684	2.7109083356858523
6	0.7625050283830809	2.080600468711941
7	0.512136542889173	1.63034131811451
8	0.40047996263763197	1.4570199836198856
9	0.308697071251947	1.2634840044899875
>10	1.7803331984905542	14.813474386445238
>50	0.1269274777161886	4.027579171305158
>100	0.10516743014193433	9.924361223489594
>500	0.009466785757751204	2.9704584417302464
>1k	0.0032703441708595063	2.2838902169907085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3730	0.2912201421279214	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2639	0.2060402024331326	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1877	0.14654697232549824	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1577	0.12312444078705952	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1520	0.11867415979475615	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1442	0.11258430159476208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1376	0.10743134465630558	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1352	0.10555754213323047	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1339	0.1045425657665648	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1331	0.10391796492553979	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1327	0.10360566450502726	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1320	0.10305913876913035	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1311	0.10235646282297718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	50	4.583864E-6	17.09104	3
CCGAACG	35	0.0021678929	16.28799	9
GCGAAAT	65	3.3674787E-6	14.616858	12
GCGTTAT	75	9.709074E-7	13.928207	1
CCTACGA	70	7.2936855E-6	13.565376	2
AAATCGC	85	2.6905946E-7	13.414688	14
GCTCGCG	50	0.0014981572	13.30134	13
TATGCCG	50	0.0015034588	13.295106	5
TAGGCCC	90	5.436923E-7	12.660028	4
ACGGTAT	245	0.0	12.409898	9
CGAAATT	85	3.937341E-6	12.295357	13
CGCGCTA	70	1.0879966E-4	12.217425	16
GTGTAGG	140	5.2750693E-11	12.209792	1
CGTTGTA	55	0.0030782053	12.085517	2
CGCGGTC	150	1.4551915E-11	12.035017	10
CGCGTAA	95	1.0375315E-6	12.001678	10
AAGACGG	320	0.0	11.870631	5
ACGCGCG	65	8.0149464E-4	11.693486	13
TAAGACA	65	8.0570794E-4	11.68618	4
AACCGCG	155	2.5465852E-11	11.645879	7
>>END_MODULE