FastQCFastQC Report
Thu 2 Feb 2017
SRR4064228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280818
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15810.12343674120757205No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15420.12039181210757502No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13480.10524524171271797No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG13460.1050890915024617No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13380.10446449066143666No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12980.10134148645631151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG407.032362E-427.510
AACGAAT1900.022.00171931
CCTAATA1004.6264176E-821.9991422
CGAATGT2050.020.39183633
GTATAGA1201.4302714E-820.1682421
GTATCAA25700.020.0326941
CAACGAA2100.019.90631730
GTCGAAC901.002036E-519.55555523
TAGGACG2650.019.0935944
GCGCATT708.1236823E-418.85714311
CGGTACG708.1236823E-418.85714338
GATATAC2350.018.7248671
AATCCCG1658.185452E-1118.66593719
CGTCGAA951.5972302E-518.52631822
AGTATCG600.007412337718.3333328
GTATCGA600.007412337718.3333329
AAGCGTA600.00741400718.3326175
GGCGTTA3350.017.7306542
TTAGGAC3150.017.4596353
CGTCGTA3550.016.73239510
Produced by FastQC (version 0.11.5)