##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1280818
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244602277606965	32.0	32.0	32.0	32.0	32.0
2	31.268506532544045	32.0	32.0	32.0	32.0	32.0
3	31.380563827179195	32.0	32.0	32.0	32.0	32.0
4	31.487158987459576	32.0	32.0	32.0	32.0	32.0
5	31.40801269188909	32.0	32.0	32.0	32.0	32.0
6	34.9807146682823	36.0	36.0	36.0	36.0	36.0
7	34.99285847013393	36.0	36.0	36.0	36.0	36.0
8	34.92496904322082	36.0	36.0	36.0	36.0	36.0
9	35.07008099511406	36.0	36.0	36.0	36.0	36.0
10	34.8914115822857	36.0	36.0	36.0	32.0	36.0
11	35.06528952591235	36.0	36.0	36.0	36.0	36.0
12	34.95961877487668	36.0	36.0	36.0	36.0	36.0
13	35.00309333566518	36.0	36.0	36.0	36.0	36.0
14	34.9427818784558	36.0	36.0	36.0	32.0	36.0
15	34.91668371306462	36.0	36.0	36.0	32.0	36.0
16	34.92656802137384	36.0	36.0	36.0	32.0	36.0
17	34.884550342047035	36.0	36.0	36.0	32.0	36.0
18	34.881199358534936	36.0	36.0	36.0	32.0	36.0
19	34.88286313902522	36.0	36.0	36.0	32.0	36.0
20	34.87644848838789	36.0	36.0	36.0	32.0	36.0
21	34.86742847149244	36.0	36.0	36.0	32.0	36.0
22	34.84974211792776	36.0	36.0	36.0	32.0	36.0
23	34.80400650209476	36.0	36.0	36.0	32.0	36.0
24	34.762467423162384	36.0	36.0	36.0	32.0	36.0
25	34.740800019987226	36.0	36.0	36.0	32.0	36.0
26	34.67781995568457	36.0	36.0	36.0	32.0	36.0
27	34.67834383963998	36.0	36.0	36.0	32.0	36.0
28	34.65420535938752	36.0	36.0	36.0	32.0	36.0
29	34.61675273145755	36.0	36.0	36.0	32.0	36.0
30	34.602122237507594	36.0	36.0	36.0	32.0	36.0
31	34.59332317315965	36.0	36.0	36.0	32.0	36.0
32	34.55260934808849	36.0	36.0	36.0	32.0	36.0
33	34.52672198548115	36.0	36.0	36.0	32.0	36.0
34	34.51104216211827	36.0	36.0	36.0	32.0	36.0
35	34.467929089066516	36.0	36.0	36.0	32.0	36.0
36	34.432448638292094	36.0	36.0	36.0	32.0	36.0
37	34.42748228085489	36.0	36.0	36.0	32.0	36.0
38	34.39036381437488	36.0	36.0	36.0	32.0	36.0
39	34.38755389134131	36.0	36.0	36.0	32.0	36.0
40	34.35559775081237	36.0	36.0	36.0	32.0	36.0
41	34.3437943564191	36.0	36.0	36.0	32.0	36.0
42	34.274384026458094	36.0	36.0	36.0	32.0	36.0
43	34.27006881539766	36.0	36.0	36.0	32.0	36.0
44	34.1813388006727	36.0	36.0	36.0	32.0	36.0
45	34.146747625345675	36.0	36.0	36.0	32.0	36.0
46	34.119264407589526	36.0	36.0	36.0	32.0	36.0
47	34.0881428899344	36.0	36.0	36.0	32.0	36.0
48	34.039203071786936	36.0	36.0	36.0	32.0	36.0
49	34.01846242010965	36.0	36.0	36.0	32.0	36.0
50	33.379949376101834	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	3.0
20	7.0
21	31.0
22	142.0
23	373.0
24	988.0
25	2397.0
26	5010.0
27	9244.0
28	15976.0
29	25245.0
30	36232.0
31	52217.0
32	76579.0
33	123262.0
34	249799.0
35	683309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70165336040133	18.139178980655487	11.675964785570672	26.483202873372502
2	16.153580697245186	20.15362129649004	36.82210972414375	26.87068828212103
3	18.12742813956103	24.012738754044296	28.914145357334696	28.945687749059974
4	12.104373923539487	15.38805669501834	36.21373216179036	36.29383721965181
5	14.241055325580994	36.86886036892049	33.11922537003696	15.770858935461558
6	34.53121645202469	36.24629630352549	15.987710949668765	13.234776294781057
7	30.318484466152828	30.560517669985384	20.411362756242895	18.709635107618894
8	28.479690322903018	33.0141362785345	18.87832619466622	19.62784720389626
9	27.552663980343674	13.317130032659048	18.30429049140036	40.825915495596924
10	15.65006722329533	26.546799437229367	31.509143461226756	26.293989878248542
11	38.05091746056036	20.771179043392582	22.179341639483518	18.99856185656354
12	24.40590654246469	23.617971966330863	28.87883280749709	23.097288683707355
13	29.458835066219763	19.244291312301346	25.573484300181683	25.72338932129721
14	23.440814545136565	19.457875583711285	24.963050812257528	32.138259058894626
15	25.378582331653348	27.298762848766167	22.117952942186896	25.20470187739359
16	25.840386818018924	25.883094200618515	23.553316692809	24.723202288553562
17	23.904879537920298	25.952243019695224	25.06413869886276	25.07873874352172
18	24.647665744077013	24.972380866869923	26.38710508543388	23.99284830361918
19	25.834019745240333	24.444826145852446	25.367129522999026	24.354024585908192
20	25.771778439859837	24.03240766765979	25.184414838133428	25.011399054346946
21	27.18567353050941	23.700010462064085	24.320239097201945	24.79407691022456
22	25.679332550499055	24.214875516858005	25.19151853193589	24.91427340070705
23	24.387827361967883	24.022666959190573	25.902497409836144	25.6870082690054
24	24.764719935915753	24.85599001884739	25.38432590524463	24.994964139992224
25	24.720643347907558	24.324875078076204	25.62484384759525	25.32963772642099
26	24.660981482811714	25.160530186858388	25.672727670219775	24.505760660110123
27	25.312729603767824	24.58806985654376	25.097775581129333	25.00142495855908
28	24.304378412521928	24.556728382578797	25.97112185645878	25.1677713484405
29	24.591443881164064	24.467770307567747	25.625648525013055	25.315137286255133
30	24.263685126915842	25.220609477111427	25.958134559678943	24.55757083629379
31	25.299596165918654	24.575006012224335	24.906459159038167	25.21893866281884
32	24.85907025406393	24.831743156514	24.774278174237573	25.5349084151845
33	24.298758944849368	24.446248921542985	25.473369431550672	25.78162270205697
34	25.13716154651547	24.405503170311423	25.59227163881014	24.865063644362966
35	25.775324078086363	24.335428139105098	25.540468477584483	24.348779305224053
36	24.479235801279597	25.215465748012438	25.141678782304243	25.16361966840372
37	25.47520204626744	24.81505280803516	25.004782275676135	24.70496287002126
38	24.644321440813417	24.679535980123696	25.554589953627683	25.121552625435207
39	25.543930604245883	24.61925248092944	24.596141383698868	25.240675531125806
40	25.422397531511947	24.9528413846519	25.36602580919187	24.25873527464428
41	24.57789690573545	25.06228582537795	25.744911925383494	24.614905343503107
42	25.81376810292934	25.40457104577526	24.869437172519383	23.912223678776016
43	24.976284409077742	24.352769317373554	25.46566489928584	25.205281374262867
44	24.539584634603372	25.03060587133042	24.976499063085573	25.453310430980636
45	24.7437925418372	25.293762170147048	25.01104779988726	24.951397488128496
46	24.822280293158464	24.82259259577345	24.985770712104618	25.369356398963465
47	24.848004084975614	24.924519300963233	25.267198161136363	24.960278452924793
48	25.4886422016343	25.526431260608657	24.320460589290704	24.66446594846634
49	24.821616928649114	25.414444441841926	24.618152092697517	25.145786536811443
50	24.733526753507952	25.661303368821457	24.441834972407957	25.16333490526263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	21.5
2	26.0
3	66.5
4	107.0
5	155.5
6	204.0
7	209.5
8	215.0
9	265.5
10	316.0
11	523.5
12	731.0
13	1366.5
14	2002.0
15	2660.5
16	3319.0
17	3651.0
18	3983.0
19	4062.5
20	4142.0
21	4564.5
22	4987.0
23	5744.0
24	6501.0
25	7150.5
26	7800.0
27	9527.5
28	11255.0
29	12919.0
30	14583.0
31	16852.0
32	19121.0
33	22631.5
34	26142.0
35	30190.0
36	34238.0
37	40004.0
38	45770.0
39	49968.5
40	54167.0
41	59732.5
42	65298.0
43	67572.5
44	69847.0
45	76061.0
46	82275.0
47	87919.5
48	93564.0
49	96618.0
50	99672.0
51	98699.0
52	97726.0
53	95086.0
54	92446.0
55	93023.0
56	93600.0
57	91129.5
58	88659.0
59	80891.0
60	73123.0
61	64297.0
62	55471.0
63	47903.0
64	40335.0
65	34380.0
66	28425.0
67	24062.0
68	19699.0
69	17988.5
70	16278.0
71	12535.5
72	8793.0
73	7457.5
74	6122.0
75	4827.5
76	3533.0
77	3031.5
78	2530.0
79	2065.0
80	1600.0
81	1294.0
82	988.0
83	796.0
84	604.0
85	444.0
86	284.0
87	211.0
88	138.0
89	102.0
90	66.0
91	55.0
92	44.0
93	31.5
94	19.0
95	21.0
96	23.0
97	18.5
98	14.0
99	15.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007260984776916002
2	4.684506307687743E-4
3	1.561502102562581E-4
4	0.0
5	0.0
6	2.3422531538438716E-4
7	1.561502102562581E-4
8	0.0
9	7.026759461531615E-4
10	9.369012615375486E-4
11	0.0
12	7.807510512812905E-5
13	5.465257358969034E-4
14	3.903755256406453E-4
15	0.00413798057179084
16	7.026759461531615E-4
17	0.0
18	2.3422531538438716E-4
19	2.3422531538438716E-4
20	7.807510512812906E-4
21	0.0
22	1.561502102562581E-4
23	8.588261564094195E-4
24	3.123004205125162E-4
25	0.001405351892306323
26	0.0048406565179440015
27	0.006167933305122195
28	0.004450280992303357
29	0.0021080278384594843
30	0.0037476050461501946
31	0.0073390598820441315
32	0.002966853994868904
33	0.00413798057179084
34	0.0022641780487157427
35	0.0024203282589720008
36	0.008119810933325422
37	0.0036695299410220658
38	0.007573285197428519
39	0.0037476050461501946
40	0.002654553574356388
41	0.0022641780487157427
42	0.001327276787178194
43	0.0011711265769219358
44	0.001405351892306323
45	0.0015615021025625812
46	7.026759461531615E-4
47	0.0021080278384594843
48	0.0018738025230750973
49	5.465257358969034E-4
50	0.0010930514717938068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1280818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.53702530054886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40756743745696	37.3546657600215
2	13.47589271270621	13.351112765136191
3	4.477788557174107	6.654489751417259
4	2.0298291252074416	4.02206786924768
5	1.0846411396243623	2.686494778779409
6	0.6798549031950222	2.020679372416441
7	0.4994339712885933	1.7318331290173647
8	0.3416588442857033	1.3539810250829736
9	0.2670164011535752	1.1904478397645544
>10	1.5362518635906863	13.823176945385034
>50	0.10595327451132454	3.616068659921586
>100	0.08458950716973232	8.427949446618472
>500	0.007617810109121859	2.576686187065959
>1k	0.0019044525272804648	1.190346470125574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1581	0.12343674120757205	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1542	0.12039181210757502	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1348	0.10524524171271797	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1346	0.1050890915024617	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1338	0.10446449066143666	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1298	0.10134148645631151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	7.807510512812905E-5	0.0
14	0.0	0.0	0.0	7.807510512812905E-5	0.0
15	0.0	0.0	0.0	7.807510512812905E-5	7.807510512812905E-5
16	0.0	0.0	0.0	7.807510512812905E-5	7.807510512812905E-5
17	0.0	0.0	0.0	7.807510512812905E-5	7.807510512812905E-5
18	0.0	0.0	0.0	7.807510512812905E-5	7.807510512812905E-5
19	0.0	0.0	0.0	7.807510512812905E-5	7.807510512812905E-5
20	0.0	0.0	0.0	1.561502102562581E-4	1.561502102562581E-4
21	0.0	0.0	0.0	1.561502102562581E-4	1.561502102562581E-4
22	0.0	0.0	0.0	3.9037552564064526E-4	1.561502102562581E-4
23	0.0	0.0	0.0	7.807510512812905E-4	1.561502102562581E-4
24	0.0	0.0	0.0	0.0010149763666656778	1.561502102562581E-4
25	0.0	0.0	0.0	0.0010930514717938068	1.561502102562581E-4
26	0.0	0.0	0.0	0.001327276787178194	2.3422531538438716E-4
27	0.0	0.0	0.0	0.0017957274179469683	3.123004205125162E-4
28	0.0	0.0	0.0	0.0035914548358939365	3.123004205125162E-4
29	0.0	0.0	0.0	0.009525162825631745	3.123004205125162E-4
30	0.0	0.0	0.0	0.02006530201792917	3.123004205125162E-4
31	0.0	0.0	0.0	0.03872525214355201	3.123004205125162E-4
32	0.0	0.0	0.0	0.05816595332045615	3.123004205125162E-4
33	0.0	0.0	0.0	0.07862163086402596	3.123004205125162E-4
34	0.0	0.0	0.0	0.10048266029990209	3.9037552564064526E-4
35	0.0	0.0	0.0	0.12390519183834081	3.9037552564064526E-4
36	0.0	0.0	0.0	0.159117064251127	3.9037552564064526E-4
37	0.0	0.0	0.0	0.21462846399722676	3.9037552564064526E-4
38	0.0	0.0	0.0	0.28958056492023065	3.9037552564064526E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAG	40	7.032362E-4	27.5	10
AACGAAT	190	0.0	22.001719	31
CCTAATA	100	4.6264176E-8	21.999142	2
CGAATGT	205	0.0	20.391836	33
GTATAGA	120	1.4302714E-8	20.168242	1
GTATCAA	2570	0.0	20.032694	1
CAACGAA	210	0.0	19.906317	30
GTCGAAC	90	1.002036E-5	19.555555	23
TAGGACG	265	0.0	19.093594	4
GCGCATT	70	8.1236823E-4	18.857143	11
CGGTACG	70	8.1236823E-4	18.857143	38
GATATAC	235	0.0	18.724867	1
AATCCCG	165	8.185452E-11	18.665937	19
CGTCGAA	95	1.5972302E-5	18.526318	22
AGTATCG	60	0.0074123377	18.333332	8
GTATCGA	60	0.0074123377	18.333332	9
AAGCGTA	60	0.007414007	18.332617	5
GGCGTTA	335	0.0	17.73065	42
TTAGGAC	315	0.0	17.459635	3
CGTCGTA	355	0.0	16.732395	10
>>END_MODULE